##################################
# NO CUT ENZYMES ( 98 enzymes) #
##################################
AatII AccII AccIII AclI AflII
AflIII AgeI AhdI Alw44I Aor51HI
ApaI ApaLI AscI AspEI AviII
AvrII BbrPI BbvCI BciVI BfrI
BglI BlnI BlpI Bpu1102I BsaAI
BsaBI BsaI BseAI BsiEI BsiWI
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrBI BsrFI BsrGI BssHII BstEII
BstPI BstUI CelII Cfr10I ClaI
CpoI DrdI EagI Eam1105I EclXI
Eco47III Eco52I EcoO65I EcoRV EcoT22I
FseI FspI KspI MamI MluI
MroI MvnI NaeI NdeI NgoMIV
NheI NotI NruI NsiI NspI
PacI PaeR7I PinAI PmaCI PmeI
PmlI PshAI Psp1406I PstI PvuI
RcaI RsrII SacII SalI SbfI
ScaI SfiI SgrAI SmlI SnaBI
SpeI SphI Sse8387I SspBI SspI
SwaI XbaI XhoI
###################################
# ONE CUT ENZYMES ( 51 enzymes) #
###################################
Acc65I 1333 GGTACC G^GTACC
AccI 2443 GTATAC GT^[AC][GT]AC
AocI 668 CCTCAGG CC^T[ACGT]AGG
AseI 1816 ATTAAT AT^TAAT
AsnI 1816 ATTAAT AT^TAAT
Asp718I 1333 GGTACC G^GTACC
AspI 1886 GACGGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 621 TGGCCA TGG^CCA
BamHI 1799 GGATCC G^GATCC
BcgI 1245 GCAGGAGATTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 1824 TGATCA T^GATCA
BglII 1628 AGATCT A^GATCT
BsaWI 1831 TCCGGT [AT]^CCGG[AT]
BsmBI 1884 GAGACG CGTCTC(1/5)
BsmFI 810 GGGAC GGGAC(10/14)
BsrDI 2154 CATTGC GCAATG(2/0)
Bst1107I 2443 GTATAC GTA^TAC
BstAPI 1943 GCAATCTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 563 TTCGAA TT^CGAA
BstXI 2022 CCAAAATGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 2443 GTATAC GTA^TAC
Bsu36I 668 CCTCAGG CC^T[ACGT]AGG
DraI 2303 TTTAAA TTT^AAA
DraIII 506 CACTCCGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 621 TGGCCA [CT]^GGCC[GA]
Eco81I 668 CCTCAGG CC^T[ACGT]AGG
EcoNI 2478 CCTATCTTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FauI 1875 CCCGC CCCGC(4/6)
FbaI 1824 TGATCA T^GATCA
HgaI 4 GCGTC GACGC(5/10)
HincII 2321 GTTAAC GT[CT]^[GA]AC
HindII 2321 GTTAAC GT[CT]^[GA]AC
HindIII 1965 AAGCTT A^AGCTT
HpaI 2321 GTTAAC GTT^AAC
KpnI 1333 GGTACC GGTAC^C
MfeI 1445 CAATTG C^AATTG
MluNI 621 TGGCCA TGG^CCA
MscI 621 TGGCCA TGG^CCA
MunI 1445 CAATTG C^AATTG
NspV 563 TTCGAA TT^CGAA
PflFI 1886 GACGGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshBI 1816 ATTAAT AT^TAAT
SacI 1129 GAGCTC GAGCT^C
SexAI 1028 ACCAGGT A^CC[AT]GGT
SfcI 638 CTGTAG C^T[GA][CT]AG
SfuI 563 TTCGAA TT^CGAA
SmaI 1956 CCCGGG CCC^GGG
StuI 2411 AGGCCT AGG^CCT
Tth111I 1886 GACGGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmaCI 1956 CCCGGG C^CCGGG
XmaI 1956 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 34 enzymes) #
###################################
AcyI 1061 GGCGCC G[GA]^CG[CT]C
1154 GGCGCC
AlwI 1800 GATCC GGATC(4/5)
1799 GGATC
AlwNI 1349 CAGGACCTG CAG[ACGT][ACGT][ACGT]^CTG
1478 CAGCTCCTG
Asp700I 309 GAAGACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1069 GAAGAACTTC
AspHI 1024 GAGCAC G[AT]GC[AT]^C
1129 GAGCTC
AvaI 1881 CCCGAG C^[CT]CG[GA]G
1956 CCCGGG
BbeI 1061 GGCGCC GGCGC^C
1154 GGCGCC
BcnI 1956 CCCGG CC^[GC]GG
1957 CCGGG
BsaHI 1061 GGCGCC G[GA]^CG[CT]C
1154 GGCGCC
BsiHKAI 1024 GAGCAC G[AT]GC[AT]^C
1129 GAGCTC
BsmI 1465 GCATTC GAATGC(1/-1)
1743 GCATTC
BsoBI 1881 CCCGAG C^[CT]CG[GA]G
1956 CCCGGG
BspMI 231 ACCTGC ACCTGC(4/8)
1353 ACCTGC
BssSI 983 CTCGTG CACGAG(-5/-1)
1997 CTCGTG
BstYI 1628 AGATCT [GA]^GATC[CT]
1799 GGATCC
EcoRI 1585 GAATTC G^AATTC
2179 GAATTC
EcoT14I 785 CCATGG C^C[AT][AT]GG
1787 CCATGG
HaeII 1061 GGCGCC [GA]GCGC^[CT]
1154 GGCGCC
Hin1I 1061 GGCGCC G[GA]^CG[CT]C
1154 GGCGCC
KasI 1061 GGCGCC G^GCGCC
1154 GGCGCC
MaeII 1509 ACGT A^CGT
2191 ACGT
MflI 1628 AGATCT [GA]^GATC[CT]
1799 GGATCC
NarI 1061 GGCGCC GG^CGCC
1154 GGCGCC
NciI 1956 CCCGG CC^[GC]GG
1957 CCGGG
NcoI 785 CCATGG C^CATGG
1787 CCATGG
PflMI 505 CCACTCCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
911 CCATGCGCTGG
PleI 2472 GACTC GAGTC(4/5)
1890 GAGTC
PpuMI 1350 AGGACCT [GA]G^G[AT]CC[CT]
1563 AGGACCC
SapI 1087 GAAGAGC GCTCTTC(1/4)
1144 GAAGAGC
SfoI 1061 GGCGCC GGC^GCC
1154 GGCGCC
StyI 785 CCATGG C^C[AT][AT]GG
1787 CCATGG
Van91I 505 CCACTCCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
911 CCATGCGCTGG
XhoII 1628 AGATCT [GA]^GATC[CT]
1799 GGATCC
XmnI 309 GAAGACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1069 GAAGAACTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 75 enzymes) #
#########################################
AciI AcsI AfaI AluI ApoI
AvaII BanI BanII BbsI BbvI
BfaI BmyI BpmI BpuAI BsaJI
BseRI BsgI BsiYI BslI BsmAI
Bsp1286I BsrI BssKI BstF5I BstNI
Cac8I CfoI Cfr13I DdeI DpnII
DraII DsaI EarI EcoO109I EcoRII
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeIII MboI MboII
MnlI MseI MslI MspA1I MspI
MvaI MwoI NdeII NlaIII NlaIV
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XcmI