##################################
# NO CUT ENZYMES ( 120 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AflII AflIII AgeI AhdI
Alw44I Aor51HI ApaLI ApoI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI AvaI AviII AvrII BamHI
BanI BbeI BbrPI BcgI BciVI
BfrI BlnI BsaWI BseAI BsiEI
BsiWI BsmBI BsoBI BspDI BspEI
BspHI BspLU11I BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstXI
BstZ17I ClaI CpoI DrdI EagI
Eam1105I EclXI Eco47III Eco52I EcoO65I
EcoRI EcoRV EcoT22I FseI FspI
HaeII HindIII HpaI KasI KpnI
KspI MfeI MluI MroI MunI
NarI NcoI NdeI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PstI PvuI RcaI
RsrII SacII SalI SapI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SwaI TaqI Tth111I TthHB8I
XbaI XhoI XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 24 enzymes) #
###################################
BalI 1572 TGGCCA TGG^CCA
BbvCI 1024 GCTGAGG CCTCAGC(-5/-2)
BglII 1405 AGATCT A^GATCT
BlpI 2031 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 2031 GCTCAGC GC^T[ACGT]AGC
BsaAI 581 TACGTG [CT]AC^GT[GA]
BseRI 1199 CTCCTC GAGGAG(10/8)
Bsp1407I 1373 TGTACA T^GTACA
BsrGI 1373 TGTACA T^GTACA
CelII 2031 GCTCAGC GC^T[ACGT]AGC
DraI 1318 TTTAAA TTT^AAA
DraIII 938 CACTTTGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 1464 CCGTGG C^C[GA][CT]GG
EcoNI 648 CCTACAAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HincII 803 GTTGAC GT[CT]^[GA]AC
HindII 803 GTTGAC GT[CT]^[GA]AC
MluNI 1572 TGGCCA TGG^CCA
MscI 1572 TGGCCA TGG^CCA
NheI 2024 GCTAGC G^CTAGC
NspI 108 ACATGC [GA]CATG^[CT]
PvuII 501 CAGCTG CAG^CTG
SacI 1186 GAGCTC GAGCT^C
SspBI 1373 TGTACA T^GTACA
XcmI 884 CCATGCAGACACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 33 enzymes) #
###################################
AcyI 335 GGCGTC G[GA]^CG[CT]C
1808 GGCGTC
AocI 809 CCTCAGG CC^T[ACGT]AGG
2183 CCTCAGG
ApaI 305 GGGCCC GGGCC^C
1479 GGGCCC
AspHI 1055 GTGCTC G[AT]GC[AT]^C
1186 GAGCTC
BbsI 769 GAAGAC GAAGAC(2/6)
1636 GTCTTC
BclI 606 TGATCA T^GATCA
682 TGATCA
BglI 10 GCCAAGGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1015 GCCCTGTGGGC
BpuAI 769 GAAGAC GAAGAC(2/6)
1636 GTCTTC
BsaBI 517 GATTGGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
601 GATGGTGATC
BsaHI 335 GGCGTC G[GA]^CG[CT]C
1808 GGCGTC
BsaI 377 GAGACC GGTCTC(1/5)
2191 GGTCTC
BsiHKAI 1055 GTGCTC G[AT]GC[AT]^C
1186 GAGCTC
BsmI 92 GCATTC GAATGC(1/-1)
1528 GCATTC
BsrBI 483 CCGCTC CCGCTC(-3/-3)
229 GAGCGG
BsrFI 260 GCCGGC [GA]^CCGG[CT]
332 GCCGGC
Bsu36I 809 CCTCAGG CC^T[ACGT]AGG
2183 CCTCAGG
Cfr10I 260 GCCGGC [GA]^CCGG[CT]
332 GCCGGC
EaeI 157 CGGCCA [CT]^GGCC[GA]
1572 TGGCCA
Eco81I 809 CCTCAGG CC^T[ACGT]AGG
2183 CCTCAGG
FbaI 606 TGATCA T^GATCA
682 TGATCA
Hin1I 335 GGCGTC G[GA]^CG[CT]C
1808 GGCGTC
MaeII 318 ACGT A^CGT
582 ACGT
MamI 517 GATTGGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
601 GATGGTGATC
NaeI 260 GCCGGC GCC^GGC
332 GCCGGC
NgoMIV 260 GCCGGC G^CCGGC
332 GCCGGC
PflMI 512 CCAGTGATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1066 CCATCCCTTGG
SfcI 918 CTACAG C^T[GA][CT]AG
1829 CTGTAG
SmlI 1242 CTTGAG C^T[CT][GA]AG
2063 CTCAAG
SspI 1323 AATATT AAT^ATT
2223 AATATT
StuI 1473 AGGCCT AGG^CCT
2069 AGGCCT
TfiI 714 GAATC G^A[AT]TC
985 GAATC
Tsp509I 1517 AATT ^AATT
1681 AATT
Van91I 512 CCAGTGATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1066 CCATCCCTTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 81 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AlwNI AvaII BanII BbvI BcnI
BfaI BmyI BpmI BsaJI BsgI
BsiYI BslI BsmAI BsmFI Bsp1286I
BspMI BsrDI BsrI BssKI BstF5I
BstNI BstUI BstYI Cac8I CfoI
Cfr13I DdeI DpnII DraII EarI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeIII HapII HgaI HhaI
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeIII MboI MboII
MflI MnlI MseI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV PleI PpuMI
RsaI Sau3AI Sau96I ScrFI SfaNI
StyI Tru9I TseI Tsp45I TspRI
XhoII