##################################
# NO CUT ENZYMES ( 75 enzymes) #
##################################
AatII AclI AflII AscI Asp700I
AvrII BalI BbrPI BbvCI BciVI
BfrI BglII BlnI BsiWI BsmI
Bsp1407I BspDI BsrDI BsrGI BssHII
Bst1107I BstAPI BstBI BstEII BstPI
BstZ17I ClaI CpoI DrdI EcoO65I
EcoRI EcoT22I FseI HincII HindII
HindIII HpaI MfeI MluI MluNI
MscI MunI NaeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PflMI PmaCI PmeI PmlI
PshAI Psp1406I PvuI RsrII SalI
ScaI SfiI SfuI SgrAI SmaI
SnaBI SpeI SspBI SwaI Van91I
XbaI XhoI XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 62 enzymes) #
###################################
Acc65I 1814 GGTACC G^GTACC
AccI 2122 GTCTAC GT^[AC][GT]AC
AccIII 260 TCCGGA T^CCGGA
AcsI 1429 GAATTT [GA]^AATT[CT]
AgeI 88 ACCGGT A^CCGGT
Alw44I 2521 GTGCAC G^TGCAC
AocI 1645 CCTGAGG CC^T[ACGT]AGG
Aor51HI 1529 AGCGCT AGC^GCT
ApaI 8 GGGCCC GGGCC^C
ApaLI 2521 GTGCAC G^TGCAC
ApoI 1429 GAATTT [GA]^AATT[CT]
AseI 2548 ATTAAT AT^TAAT
AsnI 2548 ATTAAT AT^TAAT
Asp718I 1814 GGTACC G^GTACC
BamHI 1757 GGATCC G^GATCC
BbeI 2069 GGCGCC GGCGC^C
BcgI 558 CGAGCAGTCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI 406 CTAG C^TAG
BsaAI 1474 TACGTG [CT]AC^GT[GA]
BsaBI 1293 GATGGATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 2726 GAGACC GGTCTC(1/5)
BseAI 260 TCCGGA T^CCGGA
BsiEI 43 CGGCCG CG[GA][CT]^CG
BsmBI 2482 GAGACG CGTCTC(1/5)
BspEI 260 TCCGGA T^CCGGA
BspHI 1838 TCATGA T^CATGA
BsrBI 2065 GAGCGG CCGCTC(-3/-3)
BsrFI 88 ACCGGT [GA]^CCGG[CT]
BssSI 1085 CACGAG CACGAG(-5/-1)
Bsu36I 1645 CCTGAGG CC^T[ACGT]AGG
Cfr10I 88 ACCGGT [GA]^CCGG[CT]
DraI 2886 TTTAAA TTT^AAA
EagI 43 CGGCCG C^GGCCG
EclXI 43 CGGCCG C^GGCCG
Eco47III 1529 AGCGCT AGC^GCT
Eco52I 43 CGGCCG C^GGCCG
Eco81I 1645 CCTGAGG CC^T[ACGT]AGG
EcoRV 1297 GATATC GAT^ATC
KasI 2069 GGCGCC G^GCGCC
KpnI 1814 GGTACC GGTAC^C
KspI 2769 CCGCGG CCGC^GG
MaeI 406 CTAG C^TAG
MaeII 1475 ACGT A^CGT
MamI 1293 GATGGATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 260 TCCGGA T^CCGGA
NarI 2069 GGCGCC GG^CGCC
NcoI 1904 CCATGG C^CATGG
NdeI 335 CATATG CA^TATG
PinAI 88 ACCGGT A^CCGGT
PshBI 2548 ATTAAT AT^TAAT
RcaI 1838 TCATGA T^CATGA
SacII 2769 CCGCGG CCGC^GG
SapI 608 GCTCTTC GCTCTTC(1/4)
SbfI 2031 CCTGCAGG CCTGCA^GG
SexAI 2126 ACCTGGT A^CC[AT]GGT
SfoI 2069 GGCGCC GGC^GCC
SmlI 828 CTCAAG C^T[CT][GA]AG
SphI 1866 GCATGC GCATG^C
Sse8387I 2031 CCTGCAGG CCTGCA^GG
SspI 1336 AATATT AAT^ATT
StuI 1938 AGGCCT AGG^CCT
Tsp509I 1430 AATT ^AATT
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AcyI 1174 GGCGTC G[GA]^CG[CT]C
2069 GGCGCC
AhdI 97 GACAGCGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1972 GACATCCAGTC
AspEI 97 GACAGCGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1972 GACATCCAGTC
AvaI 556 CCCGAG C^[CT]CG[GA]G
2377 CCCGAG
AviII 1583 TGCGCA TGC^GCA
2024 TGCGCA
BbsI 754 GAAGAC GAAGAC(2/6)
2316 GAAGAC
BclI 2195 TGATCA T^GATCA
2543 TGATCA
BglI 2287 GCCTCCAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
2768 GCCGCGGTGGC
BlpI 31 GCTCAGC GC^T[ACGT]AGC
180 GCTGAGC
Bpu1102I 31 GCTCAGC GC^T[ACGT]AGC
180 GCTGAGC
BpuAI 754 GAAGAC GAAGAC(2/6)
2316 GAAGAC
BsaHI 1174 GGCGTC G[GA]^CG[CT]C
2069 GGCGCC
BsoBI 556 CCCGAG C^[CT]CG[GA]G
2377 CCCGAG
CelII 31 GCTCAGC GC^T[ACGT]AGC
180 GCTGAGC
DraIII 297 CACCAAGTG CAC[ACGT][ACGT][ACGT]^GTG
1379 CACTGCGTG
Eam1105I 97 GACAGCGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1972 GACATCCAGTC
FbaI 2195 TGATCA T^GATCA
2543 TGATCA
FspI 1583 TGCGCA TGC^GCA
2024 TGCGCA
Hin1I 1174 GGCGTC G[GA]^CG[CT]C
2069 GGCGCC
PstI 2032 CTGCAG CTGCA^G
2638 CTGCAG
#########################################
# 3 OR MORE CUT ENZYMES ( 101 enzymes) #
#########################################
AccII AciI AfaI AflIII AluI
AlwI AlwNI AspHI AspI AvaII
BanI BanII BbvI BcnI BmyI
BpmI BsaJI BsaWI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
Bsp1286I BspLU11I BspMI BsrI BssKI
BstF5I BstNI BstUI BstXI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
DraII DsaI EaeI EarI EcoNI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeIII MboI MboII
MflI MnlI MseI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV NspI PflFI
PleI PpuMI PvuII RsaI SacI
Sau3AI Sau96I ScrFI SfaNI SfcI
StyI TaqI TfiI Tru9I TseI
Tsp45I TspRI Tth111I TthHB8I XcmI
XhoII