##################################
# NO CUT ENZYMES ( 93 enzymes) #
##################################
Acc65I AccIII AclI AflII AflIII
AgeI AscI AseI AsnI Asp700I
Asp718I AvrII BbrPI BbvCI BcgI
BclI BfrI BlnI BsaAI BsaI
BseAI BsiWI BsmI Bsp1407I BspDI
BspEI BspHI BspLU11I BsrBI BsrGI
BssHII Bst1107I BstAPI BstBI BstEII
BstPI BstZ17I ClaI CpoI DraI
DrdI EcoO65I EcoRV EcoT22I FbaI
FseI HincII HindII HindIII HpaI
KpnI KspI MfeI MluI MroI
MunI NaeI NdeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SacII SalI SbfI ScaI SexAI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspBI SwaI XbaI
XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 56 enzymes) #
###################################
AatII 1735 GACGTC GACGT^C
AccI 1579 GTCTAC GT^[AC][GT]AC
AccII 244 CGCG CG^CG
AcsI 1282 GAATTC [GA]^AATT[CT]
AhdI 442 GACTGCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 2028 AGCGCT AGC^GCT
ApaI 326 GGGCCC GGGCC^C
ApoI 1282 GAATTC [GA]^AATT[CT]
AspEI 442 GACTGCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 403 GACGAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 2128 CTCGAG C^[CT]CG[GA]G
AviII 865 TGCGCA TGC^GCA
BbeI 226 GGCGCC GGCGC^C
BbsI 1263 GAAGAC GAAGAC(2/6)
BciVI 1341 GGATAC GTATCC(6/5)
BlpI 2031 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 2031 GCTCAGC GC^T[ACGT]AGC
BpuAI 1263 GAAGAC GAAGAC(2/6)
BsaBI 786 GATTGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 1922 ACCGGA [AT]^CCGG[AT]
BsiEI 91 CGGCCG CG[GA][CT]^CG
BsmAI 488 GAGAC GTCTC(1/5)
BsmBI 488 GAGACG CGTCTC(1/5)
BsoBI 2128 CTCGAG C^[CT]CG[GA]G
BspMI 1043 ACCTGC ACCTGC(4/8)
BsrFI 1997 ACCGGC [GA]^CCGG[CT]
BstUI 244 CGCG CG^CG
CelII 2031 GCTCAGC GC^T[ACGT]AGC
Cfr10I 1997 ACCGGC [GA]^CCGG[CT]
EagI 91 CGGCCG C^GGCCG
Eam1105I 442 GACTGCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 91 CGGCCG C^GGCCG
Eco47III 2028 AGCGCT AGC^GCT
Eco52I 91 CGGCCG C^GGCCG
EcoRI 1282 GAATTC G^AATTC
FauI 588 GCGGG CCCGC(4/6)
FspI 865 TGCGCA TGC^GCA
KasI 226 GGCGCC G^GCGCC
MaeII 1736 ACGT A^CGT
MamI 786 GATTGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MseI 2195 TTAA T^TAA
MvnI 244 CGCG CG^CG
NarI 226 GGCGCC GG^CGCC
NcoI 746 CCATGG C^CATGG
NspI 206 ACATGC [GA]CATG^[CT]
PaeR7I 2128 CTCGAG C^TCGAG
PflFI 403 GACGAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PvuII 850 CAGCTG CAG^CTG
SfiI 429 GGCCTCACTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 226 GGCGCC GGC^GCC
SmlI 2128 CTCGAG C^T[CT][GA]AG
SspI 1460 AATATT AAT^ATT
TfiI 1767 GATTC G^A[AT]TC
Tru9I 2195 TTAA T^TAA
Tth111I 403 GACGAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 2128 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 17 enzymes) #
###################################
Alw44I 414 GTGCAC G^TGCAC
1409 GTGCAC
AocI 1195 CCTGAGG CC^T[ACGT]AGG
1880 CCTCAGG
ApaLI 414 GTGCAC G^TGCAC
1409 GTGCAC
BamHI 58 GGATCC G^GATCC
2143 GGATCC
BanI 226 GGCGCC G^G[CT][GA]CC
1080 GGTGCC
BfaI 1893 CTAG C^TAG
2064 CTAG
BglII 251 AGATCT A^GATCT
572 AGATCT
BsgI 504 CTGCAC GTGCAG(16/14)
619 GTGCAG
BssSI 481 CACGAG CACGAG(-5/-1)
1243 CACGAG
Bsu36I 1195 CCTGAGG CC^T[ACGT]AGG
1880 CCTCAGG
DraIII 276 CACTGTGTG CAC[ACGT][ACGT][ACGT]^GTG
1288 CACACTGTG
DsaI 746 CCATGG C^C[GA][CT]GG
1109 CCGTGG
Eco81I 1195 CCTGAGG CC^T[ACGT]AGG
1880 CCTCAGG
HaeII 226 GGCGCC [GA]GCGC^[CT]
2028 AGCGCT
MaeI 1893 CTAG C^TAG
2064 CTAG
MspA1I 586 CAGCGG C[AC]G^C[GT]G
850 CAGCTG
PleI 1266 GACTC GAGTC(4/5)
1524 GACTC
#########################################
# 3 OR MORE CUT ENZYMES ( 92 enzymes) #
#########################################
AciI AcyI AfaI AluI AlwI
AlwNI AspHI AvaII BalI BanII
BbvI BcnI BglI BmyI BpmI
BsaHI BsaJI BseRI BsiHKAI BsiYI
BslI BsmFI Bsp1286I BsrDI BsrI
BssKI BstF5I BstNI BstXI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
DraII EaeI EarI EcoNI EcoO109I
EcoRII EcoT14I Fnu4HI FokI HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeIII MboI MboII MflI MluNI
MnlI MscI MslI MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
PflMI PpuMI PstI RsaI SacI
SapI Sau3AI Sau96I ScrFI SfaNI
SfcI StuI StyI TaqI TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XcmI XhoII