################################## # NO CUT ENZYMES ( 75 enzymes) # ################################## AatII AccI AccIII AflII AflIII AhdI Aor51HI AscI AspEI AviII AvrII BamHI BbrPI BcgI BclI BfrI BlnI BlpI Bpu1102I BsaAI BsaBI BsaI BseAI BsiEI BsiWI BspEI BspLU11I BsrBI BssSI Bst1107I BstZ17I CelII CpoI DraIII EagI Eam1105I EclXI Eco47III Eco52I EcoRI FbaI FseI FspI HindIII HpaI KspI MamI MluI MroI NheI NotI NspI PacI PaeR7I PmaCI PmeI PmlI PpuMI PshAI PvuI RsrII SacII SalI SapI SbfI ScaI SnaBI SpeI SphI Sse8387I SspI SwaI XbaI XcmI XhoI ################################### # ONE CUT ENZYMES ( 62 enzymes) # ################################### Acc65I 2207 GGTACC G^GTACC AclI 1021 AACGTT AA^CGTT AgeI 20 ACCGGT A^CCGGT Alw44I 975 GTGCAC G^TGCAC AocI 207 CCTGAGG CC^T[ACGT]AGG ApaI 96 GGGCCC GGGCC^C ApaLI 975 GTGCAC G^TGCAC AseI 1398 ATTAAT AT^TAAT AsnI 1398 ATTAAT AT^TAAT Asp700I 1846 GAATGCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC Asp718I 2207 GGTACC G^GTACC AspI 2471 GACTCCGTC GAC[ACGT]^[ACGT][ACGT]GTC BalI 2289 TGGCCA TGG^CCA BciVI 2226 GTATCC GTATCC(6/5) BglII 1617 AGATCT A^GATCT BsaWI 20 ACCGGT [AT]^CCGG[AT] BspDI 403 ATCGAT AT^CGAT BssHII 57 GCGCGC G^CGCGC BstAPI 1562 GCATACCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstBI 592 TTCGAA TT^CGAA BstEII 1217 GGTGACC G^GT[ACGT]ACC BstPI 1217 GGTGACC G^GT[ACGT]ACC BstXI 2231 CCAGCACTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Bsu36I 207 CCTGAGG CC^T[ACGT]AGG ClaI 403 ATCGAT AT^CGAT DraI 2159 TTTAAA TTT^AAA DraII 96 GGGCCCC [GA]G^G[ACGT]CC[CT] DrdI 1381 GACCAACTTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EarI 1065 GAAGAG CTCTTC(1/4) Eco81I 207 CCTGAGG CC^T[ACGT]AGG EcoNI 1806 CCTAGCTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO109I 96 GGGCCCC [GA]G^G[ACGT]CC[CT] EcoO65I 1217 GGTGACC G^GT[ACGT]ACC EcoRV 1591 GATATC GAT^ATC HincII 1480 GTCAAC GT[CT]^[GA]AC HindII 1480 GTCAAC GT[CT]^[GA]AC KpnI 2207 GGTACC GGTAC^C Ksp632I 1065 GAAGAG CTCTTC(1/4) MaeII 1022 ACGT A^CGT MfeI 1294 CAATTG C^AATTG MluNI 2289 TGGCCA TGG^CCA MscI 2289 TGGCCA TGG^CCA MunI 1294 CAATTG C^AATTG NaeI 310 GCCGGC GCC^GGC NcoI 1213 CCATGG C^CATGG NgoMIV 310 GCCGGC G^CCGGC NruI 125 TCGCGA TCG^CGA NspV 592 TTCGAA TT^CGAA PflFI 2471 GACTCCGTC GAC[ACGT]^[ACGT][ACGT]GTC PflMI 1953 CCACCTGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PinAI 20 ACCGGT A^CCGGT PshBI 1398 ATTAAT AT^TAAT Psp1406I 1021 AACGTT AA^CGTT PvuII 961 CAGCTG CAG^CTG SacI 272 GAGCTC GAGCT^C SexAI 430 ACCAGGT A^CC[AT]GGT SfiI 137 GGCCCGCGCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SfuI 592 TTCGAA TT^CGAA StuI 1125 AGGCCT AGG^CCT Tth111I 2471 GACTCCGTC GAC[ACGT]^[ACGT][ACGT]GTC Van91I 1953 CCACCTGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XmnI 1846 GAATGCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 24 enzymes) # ################################### AvaII 1380 GGACC G^G[AT]CC 1689 GGTCC BbvCI 1776 CCTCAGC CCTCAGC(-5/-2) 2246 GCTGAGG BglI 138 GCCCGCGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 2453 GCCTGGGCGGC BsmFI 390 GGGAC GGGAC(10/14) 2092 GGGAC BsmI 1846 GAATGC GAATGC(1/-1) 1850 GCATTC BspHI 788 TCATGA T^CATGA 2113 TCATGA BspMI 555 GCAGGT ACCTGC(4/8) 971 GCAGGT BsrDI 2441 CATTGC GCAATG(2/0) 991 GCAATG BsrFI 20 ACCGGT [GA]^CCGG[CT] 310 GCCGGC BstYI 807 GGATCT [GA]^GATC[CT] 1617 AGATCT Cfr10I 20 ACCGGT [GA]^CCGG[CT] 310 GCCGGC EaeI 62 CGGCCA [CT]^GGCC[GA] 2289 TGGCCA EcoT14I 1213 CCATGG C^C[AT][AT]GG 1741 CCTTGG HgaI 89 GCGTC GACGC(5/10) 1944 GCGTC MflI 807 GGATCT [GA]^GATC[CT] 1617 AGATCT PleI 2471 GACTC GAGTC(4/5) 122 GAGTC RcaI 788 TCATGA T^CATGA 2113 TCATGA SgrAI 19 CACCGGTG C[GA]^CCGG[CT]G 309 CGCCGGCG SmaI 325 CCCGGG CCC^GGG 2404 CCCGGG SmlI 730 CTCAAG C^T[CT][GA]AG 1870 CTCAAG StyI 1213 CCATGG C^C[AT][AT]GG 1741 CCTTGG XhoII 807 GGATCT [GA]^GATC[CT] 1617 AGATCT XmaCI 325 CCCGGG C^CCGGG 2404 CCCGGG XmaI 325 CCCGGG C^CCGGG 2404 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 97 enzymes) # ######################################### AccII AciI AcsI AcyI AfaI AluI AlwI AlwNI ApoI AspHI AvaI BanI BanII BbeI BbsI BbvI BcnI BfaI BmyI BpmI BpuAI BsaHI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmBI BsoBI Bsp1286I Bsp1407I BsrGI BsrI BssKI BstF5I BstNI BstUI Cac8I CfoI Cfr13I DdeI DpnII DsaI EcoRII EcoT22I FauI Fnu4HI FokI HaeII HaeIII HapII HhaI Hin1I HinP1I HinfI HpaII HphI ItaI KasI MaeI MaeIII MboI MboII MnlI MseI MslI MspA1I MspI MvaI MvnI MwoI NarI NciI NdeI NdeII NlaIII NlaIV NsiI PstI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SfoI SspBI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I