##################################
# NO CUT ENZYMES ( 75 enzymes) #
##################################
AatII AccI AccIII AflII AflIII
AhdI Aor51HI AscI AspEI AviII
AvrII BamHI BbrPI BcgI BclI
BfrI BlnI BlpI Bpu1102I BsaAI
BsaBI BsaI BseAI BsiEI BsiWI
BspEI BspLU11I BsrBI BssSI Bst1107I
BstZ17I CelII CpoI DraIII EagI
Eam1105I EclXI Eco47III Eco52I EcoRI
FbaI FseI FspI HindIII HpaI
KspI MamI MluI MroI NheI
NotI NspI PacI PaeR7I PmaCI
PmeI PmlI PpuMI PshAI PvuI
RsrII SacII SalI SapI SbfI
ScaI SnaBI SpeI SphI Sse8387I
SspI SwaI XbaI XcmI XhoI
###################################
# ONE CUT ENZYMES ( 62 enzymes) #
###################################
Acc65I 2207 GGTACC G^GTACC
AclI 1021 AACGTT AA^CGTT
AgeI 20 ACCGGT A^CCGGT
Alw44I 975 GTGCAC G^TGCAC
AocI 207 CCTGAGG CC^T[ACGT]AGG
ApaI 96 GGGCCC GGGCC^C
ApaLI 975 GTGCAC G^TGCAC
AseI 1398 ATTAAT AT^TAAT
AsnI 1398 ATTAAT AT^TAAT
Asp700I 1846 GAATGCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 2207 GGTACC G^GTACC
AspI 2471 GACTCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 2289 TGGCCA TGG^CCA
BciVI 2226 GTATCC GTATCC(6/5)
BglII 1617 AGATCT A^GATCT
BsaWI 20 ACCGGT [AT]^CCGG[AT]
BspDI 403 ATCGAT AT^CGAT
BssHII 57 GCGCGC G^CGCGC
BstAPI 1562 GCATACCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 592 TTCGAA TT^CGAA
BstEII 1217 GGTGACC G^GT[ACGT]ACC
BstPI 1217 GGTGACC G^GT[ACGT]ACC
BstXI 2231 CCAGCACTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 207 CCTGAGG CC^T[ACGT]AGG
ClaI 403 ATCGAT AT^CGAT
DraI 2159 TTTAAA TTT^AAA
DraII 96 GGGCCCC [GA]G^G[ACGT]CC[CT]
DrdI 1381 GACCAACTTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 1065 GAAGAG CTCTTC(1/4)
Eco81I 207 CCTGAGG CC^T[ACGT]AGG
EcoNI 1806 CCTAGCTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 96 GGGCCCC [GA]G^G[ACGT]CC[CT]
EcoO65I 1217 GGTGACC G^GT[ACGT]ACC
EcoRV 1591 GATATC GAT^ATC
HincII 1480 GTCAAC GT[CT]^[GA]AC
HindII 1480 GTCAAC GT[CT]^[GA]AC
KpnI 2207 GGTACC GGTAC^C
Ksp632I 1065 GAAGAG CTCTTC(1/4)
MaeII 1022 ACGT A^CGT
MfeI 1294 CAATTG C^AATTG
MluNI 2289 TGGCCA TGG^CCA
MscI 2289 TGGCCA TGG^CCA
MunI 1294 CAATTG C^AATTG
NaeI 310 GCCGGC GCC^GGC
NcoI 1213 CCATGG C^CATGG
NgoMIV 310 GCCGGC G^CCGGC
NruI 125 TCGCGA TCG^CGA
NspV 592 TTCGAA TT^CGAA
PflFI 2471 GACTCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 1953 CCACCTGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 20 ACCGGT A^CCGGT
PshBI 1398 ATTAAT AT^TAAT
Psp1406I 1021 AACGTT AA^CGTT
PvuII 961 CAGCTG CAG^CTG
SacI 272 GAGCTC GAGCT^C
SexAI 430 ACCAGGT A^CC[AT]GGT
SfiI 137 GGCCCGCGCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfuI 592 TTCGAA TT^CGAA
StuI 1125 AGGCCT AGG^CCT
Tth111I 2471 GACTCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 1953 CCACCTGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI 1846 GAATGCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AvaII 1380 GGACC G^G[AT]CC
1689 GGTCC
BbvCI 1776 CCTCAGC CCTCAGC(-5/-2)
2246 GCTGAGG
BglI 138 GCCCGCGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
2453 GCCTGGGCGGC
BsmFI 390 GGGAC GGGAC(10/14)
2092 GGGAC
BsmI 1846 GAATGC GAATGC(1/-1)
1850 GCATTC
BspHI 788 TCATGA T^CATGA
2113 TCATGA
BspMI 555 GCAGGT ACCTGC(4/8)
971 GCAGGT
BsrDI 2441 CATTGC GCAATG(2/0)
991 GCAATG
BsrFI 20 ACCGGT [GA]^CCGG[CT]
310 GCCGGC
BstYI 807 GGATCT [GA]^GATC[CT]
1617 AGATCT
Cfr10I 20 ACCGGT [GA]^CCGG[CT]
310 GCCGGC
EaeI 62 CGGCCA [CT]^GGCC[GA]
2289 TGGCCA
EcoT14I 1213 CCATGG C^C[AT][AT]GG
1741 CCTTGG
HgaI 89 GCGTC GACGC(5/10)
1944 GCGTC
MflI 807 GGATCT [GA]^GATC[CT]
1617 AGATCT
PleI 2471 GACTC GAGTC(4/5)
122 GAGTC
RcaI 788 TCATGA T^CATGA
2113 TCATGA
SgrAI 19 CACCGGTG C[GA]^CCGG[CT]G
309 CGCCGGCG
SmaI 325 CCCGGG CCC^GGG
2404 CCCGGG
SmlI 730 CTCAAG C^T[CT][GA]AG
1870 CTCAAG
StyI 1213 CCATGG C^C[AT][AT]GG
1741 CCTTGG
XhoII 807 GGATCT [GA]^GATC[CT]
1617 AGATCT
XmaCI 325 CCCGGG C^CCGGG
2404 CCCGGG
XmaI 325 CCCGGG C^CCGGG
2404 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 97 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AluI AlwI AlwNI ApoI AspHI
AvaI BanI BanII BbeI BbsI
BbvI BcnI BfaI BmyI BpmI
BpuAI BsaHI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmBI
BsoBI Bsp1286I Bsp1407I BsrGI BsrI
BssKI BstF5I BstNI BstUI Cac8I
CfoI Cfr13I DdeI DpnII DsaI
EcoRII EcoT22I FauI Fnu4HI FokI
HaeII HaeIII HapII HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
KasI MaeI MaeIII MboI MboII
MnlI MseI MslI MspA1I MspI
MvaI MvnI MwoI NarI NciI
NdeI NdeII NlaIII NlaIV NsiI
PstI RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SfoI SspBI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I