##################################
# NO CUT ENZYMES ( 130 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AflII AflIII AgeI AhdI Alw44I
AocI Aor51HI ApaI ApaLI AscI
Asp718I AspEI AspI AvaI AviII
AvrII BalI BamHI BanII BbrPI
BbsI BbvCI BcgI BciVI BclI
BfrI BglI BlnI BlpI Bpu1102I
BpuAI BsaAI BsaBI BsaWI BseAI
BsiEI BsiWI BsoBI BspEI BspLU11I
BsrBI BsrFI BssHII BssSI BstAPI
BstBI BstEII BstPI BstUI Bsu36I
CelII Cfr10I CpoI DraIII DrdI
DsaI EaeI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoNI EcoO65I
EcoRI EcoRV EcoT22I FbaI FseI
FspI HgaI HincII HindII HpaI
KpnI KspI MamI MfeI MluI
MluNI MroI MscI MslI MunI
MvnI NaeI NcoI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PshAI Psp1406I PvuI
RsrII SacI SacII SalI ScaI
SexAI SfiI SfuI SgrAI SmaI
SnaBI SpeI StuI SwaI Tth111I
Van91I XbaI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 51 enzymes) #
###################################
AccI 145 GTATAC GT^[AC][GT]AC
AcyI 49 GGCGCC G[GA]^CG[CT]C
AlwNI 1019 CAGGCCCTG CAG[ACGT][ACGT][ACGT]^CTG
AseI 493 ATTAAT AT^TAAT
AsnI 493 ATTAAT AT^TAAT
Asp700I 1669 GAATCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 1028 GTGCTC G[AT]GC[AT]^C
AvaII 1171 GGTCC G^G[AT]CC
BbeI 49 GGCGCC GGCGC^C
BcnI 46 CCGGG CC^[GC]GG
BglII 375 AGATCT A^GATCT
BmyI 1028 GTGCTC G[AGT]GC[ACT]^C
BsaHI 49 GGCGCC G[GA]^CG[CT]C
BsgI 344 CTGCAC GTGCAG(16/14)
BsiHKAI 1028 GTGCTC G[AT]GC[AT]^C
BsmBI 65 GAGACG CGTCTC(1/5)
BsmI 573 GCATTC GAATGC(1/-1)
Bsp1286I 1028 GTGCTC G[AGT]GC[ACT]^C
BspDI 864 ATCGAT AT^CGAT
BspHI 156 TCATGA T^CATGA
BsrDI 59 GCAATG GCAATG(2/0)
Bst1107I 145 GTATAC GTA^TAC
BstXI 630 CCAAGTCTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 145 GTATAC GTA^TAC
ClaI 864 ATCGAT AT^CGAT
DraI 437 TTTAAA TTT^AAA
EcoT14I 366 CCTTGG C^C[AT][AT]GG
FauI 2 GCGGG CCCGC(4/6)
HaeII 49 GGCGCC [GA]GCGC^[CT]
HapII 46 CCGG C^CGG
Hin1I 49 GGCGCC G[GA]^CG[CT]C
HindIII 237 AAGCTT A^AGCTT
HpaII 46 CCGG C^CGG
KasI 49 GGCGCC G^GCGCC
MaeII 218 ACGT A^CGT
MspA1I 93 CAGCTG C[AC]G^C[GT]G
MspI 46 CCGG C^CGG
NarI 49 GGCGCC GG^CGCC
NciI 46 CCGGG CC^[GC]GG
NdeI 1577 CATATG CA^TATG
NspI 312 GCATGC [GA]CATG^[CT]
PpuMI 1170 GGGTCCC [GA]G^G[AT]CC[CT]
PshBI 493 ATTAAT AT^TAAT
PvuII 93 CAGCTG CAG^CTG
RcaI 156 TCATGA T^CATGA
SapI 641 GAAGAGC GCTCTTC(1/4)
SfoI 49 GGCGCC GGC^GCC
SphI 312 GCATGC GCATG^C
SspI 668 AATATT AAT^ATT
StyI 366 CCTTGG C^C[AT][AT]GG
XmnI 1669 GAATCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AcsI 1353 AAATTC [GA]^AATT[CT]
1440 AAATTC
AlwI 508 GATCC GGATC(4/5)
1371 GATCC
ApoI 1353 AAATTC [GA]^AATT[CT]
1440 AAATTC
BanI 49 GGCGCC G^G[CT][GA]CC
656 GGCACC
BpmI 425 CTCCAG CTGGAG(16/14)
914 CTGGAG
BsaI 119 GAGACC GGTCTC(1/5)
917 GAGACC
BsmFI 220 GTCCC GGGAC(10/14)
1172 GTCCC
Bsp1407I 552 TGTACA T^GTACA
721 TGTACA
BspMI 900 ACCTGC ACCTGC(4/8)
226 GCAGGT
BsrGI 552 TGTACA T^GTACA
721 TGTACA
CfoI 50 GCGC GCG^C
571 GCGC
HhaI 50 GCGC GCG^C
571 GCGC
HinP1I 50 GCGC G^CGC
571 GCGC
PstI 224 CTGCAG CTGCA^G
1016 CTGCAG
SbfI 223 CCTGCAGG CCTGCA^GG
1015 CCTGCAGG
Sse8387I 223 CCTGCAGG CCTGCA^GG
1015 CCTGCAGG
SspBI 552 TGTACA T^GTACA
721 TGTACA
TaqI 730 TCGA T^CGA
865 TCGA
TthHB8I 730 TCGA T^CGA
865 TCGA
XcmI 666 CCAATATTGCTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1519 CCATAATGTGAGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 57 enzymes) #
#########################################
AciI AfaI AluI BbvI BfaI
BsaJI BseRI BsiYI BslI BsmAI
BsrI BssKI BstF5I BstNI BstYI
Cac8I Cfr13I DdeI DpnII DraII
EarI EcoO109I EcoRII Fnu4HI FokI
HaeIII HinfI HphI ItaI Ksp632I
MaeI MaeIII MboI MboII MflI
MnlI MseI MvaI MwoI NdeII
NlaIII NlaIV PleI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI XhoII