##################################
# NO CUT ENZYMES ( 98 enzymes) #
##################################
AatII Acc65I AccIII AclI AgeI
AocI Aor51HI ApaI AscI Asp700I
Asp718I AvaI AviII BbrPI BciVI
BclI BglII BlpI Bpu1102I BsaBI
BsaWI BseAI BsiEI BsiWI BsmI
BsoBI Bsp1407I BspDI BspEI BspHI
BsrDI BsrGI Bst1107I BstBI BstZ17I
Bsu36I CelII ClaI CpoI DraI
DraIII DrdI EagI EclXI Eco47III
Eco52I Eco81I EcoRI EcoRV EcoT22I
FbaI FseI FspI HgaI HindIII
HpaI KpnI MamI MfeI MluI
MroI MunI NdeI NheI NotI
NruI NsiI NspV PacI PaeR7I
PflMI PinAI PmaCI PmeI PmlI
PshAI Psp1406I PvuI RcaI RsrII
SapI ScaI SexAI SfiI SfuI
SgrAI SmaI SnaBI SpeI SphI
SspBI SspI SwaI Van91I XhoI
XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 41 enzymes) #
###################################
AflII 843 CTTAAG C^TTAAG
AflIII 1759 ACATGT A^C[GA][CT]GT
AhdI 1620 GACTCCAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 327 GTGCAC G^TGCAC
ApaLI 327 GTGCAC G^TGCAC
AseI 351 ATTAAT AT^TAAT
AsnI 351 ATTAAT AT^TAAT
AspEI 1620 GACTCCAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 1076 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 1657 GGATCC G^GATCC
BcgI 530 GCAGGGGCCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI 843 CTTAAG C^TTAAG
BsaAI 1203 TACGTG [CT]AC^GT[GA]
BsaI 2096 GAGACC GGTCTC(1/5)
BsmBI 1100 CGTCTC CGTCTC(1/5)
BspLU11I 1759 ACATGT A^CATGT
BsrBI 580 GAGCGG CCGCTC(-3/-3)
BssHII 181 GCGCGC G^CGCGC
BssSI 594 CACGAG CACGAG(-5/-1)
BstEII 824 GGTAACC G^GT[ACGT]ACC
BstPI 824 GGTAACC G^GT[ACGT]ACC
BstYI 1657 GGATCC [GA]^GATC[CT]
Eam1105I 1620 GACTCCAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 824 GGTAACC G^GT[ACGT]ACC
HincII 255 GTCGAC GT[CT]^[GA]AC
HindII 255 GTCGAC GT[CT]^[GA]AC
KspI 161 CCGCGG CCGC^GG
MflI 1657 GGATCC [GA]^GATC[CT]
NaeI 199 GCCGGC GCC^GGC
NcoI 1217 CCATGG C^CATGG
NgoMIV 199 GCCGGC G^CCGGC
PflFI 1076 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshBI 351 ATTAAT AT^TAAT
SacI 1272 GAGCTC GAGCT^C
SacII 161 CCGCGG CCGC^GG
SalI 255 GTCGAC G^TCGAC
SbfI 940 CCTGCAGG CCTGCA^GG
Sse8387I 940 CCTGCAGG CCTGCA^GG
Tth111I 1076 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 406 CCACCATGAACTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 1657 GGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AccI 255 GTCGAC GT^[AC][GT]AC
2007 GTCTAC
AcsI 620 GAATTT [GA]^AATT[CT]
997 AAATTC
AlwNI 465 CAGCTCCTG CAG[ACGT][ACGT][ACGT]^CTG
1150 CAGTCACTG
ApoI 620 GAATTT [GA]^AATT[CT]
997 AAATTC
AvrII 1136 CCTAGG C^CTAGG
1999 CCTAGG
BalI 1088 TGGCCA TGG^CCA
1838 TGGCCA
BanII 226 GGGCTC G[GA]GC[CT]^C
1272 GAGCTC
BbsI 792 GAAGAC GAAGAC(2/6)
1927 GAAGAC
BlnI 1136 CCTAGG C^CTAGG
1999 CCTAGG
BpuAI 792 GAAGAC GAAGAC(2/6)
1927 GAAGAC
BspMI 943 GCAGGT ACCTGC(4/8)
953 GCAGGT
BsrFI 199 GCCGGC [GA]^CCGG[CT]
516 GCCGGT
Cfr10I 199 GCCGGC [GA]^CCGG[CT]
516 GCCGGT
EaeI 1088 TGGCCA [CT]^GGCC[GA]
1838 TGGCCA
EarI 984 CTCTTC CTCTTC(1/4)
1848 CTCTTC
EcoNI 749 CCTGGAGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1718 CCTACCAGAGG
Ksp632I 984 CTCTTC CTCTTC(1/4)
1848 CTCTTC
MaeII 724 ACGT A^CGT
1204 ACGT
MluNI 1088 TGGCCA TGG^CCA
1838 TGGCCA
MscI 1088 TGGCCA TGG^CCA
1838 TGGCCA
MslI 381 CACTCCCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1223 CATTGTGGTG
NspI 147 GCATGT [GA]CATG^[CT]
1759 ACATGT
TfiI 1122 GAATC G^A[AT]TC
1465 GAATC
Tsp509I 621 AATT ^AATT
998 AATT
XbaI 1491 TCTAGA T^CTAGA
1752 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 94 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AspHI AvaII BanI BbeI
BbvCI BbvI BcnI BfaI BglI
BmyI BpmI BsaHI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI Bsp1286I BsrI BssKI BstAPI
BstF5I BstNI BstUI BstXI Cac8I
CfoI Cfr13I DdeI DpnII DraII
DsaI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI KasI MaeI MaeIII
MboI MboII MnlI MseI MspA1I
MspI MvaI MvnI MwoI NarI
NciI NdeII NlaIII NlaIV PleI
PpuMI PstI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SfoI
SmlI StuI StyI TaqI Tru9I
TseI Tsp45I TspRI TthHB8I