##################################
# NO CUT ENZYMES ( 131 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcyI AflIII AgeI AhdI Alw44I
AocI Aor51HI ApaI ApaLI AscI
AseI AsnI Asp718I AspEI AspI
AviII AvrII BalI BamHI BbeI
BbrPI BbvCI BcgI BciVI BglI
BlnI BsaAI BsaHI BseAI BsiEI
BsiWI BsmI Bsp1407I BspDI BspEI
BspHI BspLU11I BspMI BsrGI BssHII
BssSI Bst1107I BstBI BstEII BstPI
BstZ17I Bsu36I ClaI CpoI DraII
DraIII DrdI EaeI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoO109I
EcoO65I EcoRI EcoRV EcoT22I FseI
FspI HaeII Hin1I HindIII HpaI
KasI KpnI MaeII MfeI MluI
MluNI MroI MscI MslI MunI
NaeI NarI NdeI NgoMIV NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SalI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SphI Sse8387I SspBI SspI SwaI
Tth111I Van91I XcmI XhoI XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 41 enzymes) #
###################################
AflII 1598 CTTAAG C^TTAAG
AlwI 60 GGATC GGATC(4/5)
Asp700I 492 GAAACAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI 171 CCCGAG C^[CT]CG[GA]G
AvaII 66 GGACC G^G[AT]CC
BanI 1888 GGCACC G^G[CT][GA]CC
BanII 237 GAGCTC G[GA]GC[CT]^C
BbsI 1388 GTCTTC GAAGAC(2/6)
BclI 584 TGATCA T^GATCA
BfrI 1598 CTTAAG C^TTAAG
BglII 956 AGATCT A^GATCT
BlpI 632 GCTAAGC GC^T[ACGT]AGC
Bpu1102I 632 GCTAAGC GC^T[ACGT]AGC
BpuAI 1388 GTCTTC GAAGAC(2/6)
BsaBI 1563 GATTACTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 1798 ACCGGA [AT]^CCGG[AT]
BsmFI 65 GGGAC GGGAC(10/14)
BsoBI 171 CCCGAG C^[CT]CG[GA]G
BsrBI 86 GAGCGG CCGCTC(-3/-3)
BsrDI 784 GCAATG GCAATG(2/0)
BsrFI 131 GCCGGT [GA]^CCGG[CT]
BstXI 1766 CCAGCACTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 956 AGATCT [GA]^GATC[CT]
CelII 632 GCTAAGC GC^T[ACGT]AGC
Cfr10I 131 GCCGGT [GA]^CCGG[CT]
DraI 1304 TTTAAA TTT^AAA
EcoT14I 468 CCATGG C^C[AT][AT]GG
FbaI 584 TGATCA T^GATCA
HgaI 706 GCGTC GACGC(5/10)
KspI 69 CCGCGG CCGC^GG
MamI 1563 GATTACTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 956 AGATCT [GA]^GATC[CT]
NcoI 468 CCATGG C^CATGG
SacI 237 GAGCTC GAGCT^C
SacII 69 CCGCGG CCGC^GG
SpeI 980 ACTAGT A^CTAGT
StuI 1754 AGGCCT AGG^CCT
StyI 468 CCATGG C^C[AT][AT]GG
XbaI 1205 TCTAGA T^CTAGA
XhoII 956 AGATCT [GA]^GATC[CT]
XmnI 492 GAAACAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 17 enzymes) #
###################################
AlwNI 316 CAGTTGCTG CAG[ACGT][ACGT][ACGT]^CTG
1084 CAGAATCTG
AspHI 40 GTGCTC G[AT]GC[AT]^C
237 GAGCTC
BcnI 98 CCGGG CC^[GC]GG
1873 CCGGG
BsgI 1964 CTGCAC GTGCAG(16/14)
1731 GTGCAG
BsiHKAI 40 GTGCTC G[AT]GC[AT]^C
237 GAGCTC
BsmBI 707 CGTCTC CGTCTC(1/5)
186 GAGACG
CfoI 14 GCGC GCG^C
1958 GCGC
EcoNI 242 CCTCCACCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
944 CCTAAATCAGG
HhaI 14 GCGC GCG^C
1958 GCGC
HinP1I 14 GCGC G^CGC
1958 GCGC
HincII 474 GTTGAC GT[CT]^[GA]AC
1212 GTTGAC
HindII 474 GTTGAC GT[CT]^[GA]AC
1212 GTTGAC
NciI 98 CCGGG CC^[GC]GG
1873 CCGGG
NlaIV 65 GGGACC GG[ACGT]^[ACGT]CC
1888 GGCACC
PvuII 457 CAGCTG CAG^CTG
523 CAGCTG
SapI 429 GAAGAGC GCTCTTC(1/4)
779 GAAGAGC
Tsp45I 547 GTGAC ^GT[GC]AC
1080 GTGAC
#########################################
# 3 OR MORE CUT ENZYMES ( 69 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
ApoI BbvI BfaI BmyI BpmI
BsaI BsaJI BseRI BsiYI BslI
BsmAI Bsp1286I BsrI BssKI BstAPI
BstF5I BstNI BstUI Cac8I Cfr13I
DdeI DpnII DsaI EarI EcoRII
FauI Fnu4HI FokI HaeIII HapII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeIII MboI MboII MnlI
MseI MspA1I MspI MvaI MvnI
MwoI NdeII NlaIII PleI PstI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI TaqI TfiI Tru9I
TseI Tsp509I TspRI TthHB8I