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# NO CUT ENZYMES ( 151 enzymes) #
##################################
AatII Acc65I AccII AccIII AcyI
AflII AflIII AgeI AhdI Alw44I
AocI Aor51HI ApaI ApaLI AscI
AseI AsnI Asp718I AspEI AspI
AvaI AviII BanII BbeI BbrPI
BbsI BbvCI BcgI BciVI BclI
BcnI BfrI BglI BlpI Bpu1102I
BpuAI BsaAI BsaHI BsaI BsaWI
BseAI BsgI BsiEI BsiWI BsmBI
BsmI BsoBI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrBI BsrDI BsrFI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstUI BstXI BstZ17I Bsu36I
CelII CfoI Cfr10I ClaI CpoI
DraIII DrdI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoRV EcoT22I
FauI FbaI FseI FspI HaeII
HapII HhaI Hin1I HinP1I HincII
HindII HindIII HpaI HpaII KasI
KpnI KspI MfeI MluI MroI
MslI MspI MunI MvnI NaeI
NarI NciI NgoMIV NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PshAI PshBI PvuI
RcaI RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspBI StuI
SwaI Tth111I Van91I XhoI XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 28 enzymes) #
###################################
AccI 1293 GTCTAC GT^[AC][GT]AC
AclI 1466 AACGTT AA^CGTT
Asp700I 762 GAATGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvrII 1439 CCTAGG C^CTAGG
BalI 1482 TGGCCA TGG^CCA
BlnI 1439 CCTAGG C^CTAGG
BsaBI 418 GATAGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmAI 1341 GAGAC GTCTC(1/5)
BspMI 312 ACCTGC ACCTGC(4/8)
BstEII 487 GGTGACC G^GT[ACGT]ACC
BstPI 487 GGTGACC G^GT[ACGT]ACC
DraI 1728 TTTAAA TTT^AAA
EaeI 1482 TGGCCA [CT]^GGCC[GA]
EarI 1519 CTCTTC CTCTTC(1/4)
EcoO65I 487 GGTGACC G^GT[ACGT]ACC
EcoRI 408 GAATTC G^AATTC
Ksp632I 1519 CTCTTC CTCTTC(1/4)
MaeII 1467 ACGT A^CGT
MamI 418 GATAGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 1482 TGGCCA TGG^CCA
MscI 1482 TGGCCA TGG^CCA
NdeI 1665 CATATG CA^TATG
Psp1406I 1466 AACGTT AA^CGTT
SmlI 571 CTCAAG C^T[CT][GA]AG
SspI 1088 AATATT AAT^ATT
Tsp45I 488 GTGAC ^GT[GC]AC
XbaI 1617 TCTAGA T^CTAGA
XmnI 762 GAATGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 16 enzymes) #
###################################
AfaI 304 GTAC GT^AC
884 GTAC
AlwNI 822 CAGATACTG CAG[ACGT][ACGT][ACGT]^CTG
1226 CAGGTCCTG
AspHI 82 GTGCTC G[AT]GC[AT]^C
1022 GTGCTC
BamHI 1266 GGATCC G^GATCC
1360 GGATCC
BanI 926 GGTGCC G^G[CT][GA]CC
1055 GGTGCC
BglII 985 AGATCT A^GATCT
1759 AGATCT
BsiHKAI 82 GTGCTC G[AT]GC[AT]^C
1022 GTGCTC
BsmFI 449 GGGAC GGGAC(10/14)
1247 GGGAC
EcoNI 455 CCTCCAGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
674 CCTCACACAGG
HgaI 1121 GACGC GACGC(5/10)
997 GCGTC
MaeIII 488 GTGAC ^GT[ACGT]AC
1075 GTTAC
NcoI 930 CCATGG C^CATGG
1237 CCATGG
PpuMI 1227 AGGTCCT [GA]G^G[AT]CC[CT]
1442 AGGTCCT
RsaI 304 GTAC GT^AC
884 GTAC
TaqI 498 TCGA T^CGA
643 TCGA
TthHB8I 498 TCGA T^CGA
643 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 63 enzymes) #
#########################################
AciI AcsI AluI AlwI ApoI
AvaII BbvI BfaI BmyI BpmI
BsaJI BseRI BsiYI BslI Bsp1286I
BsrI BssKI BstF5I BstNI BstYI
Cac8I Cfr13I DdeI DpnII DraII
DsaI EcoO109I EcoRII EcoT14I Fnu4HI
FokI HaeIII HinfI HphI ItaI
MaeI MboI MboII MflI MnlI
MseI MspA1I MvaI MwoI NdeII
NlaIII NlaIV PleI PstI PvuII
Sau3AI Sau96I ScrFI SfaNI SfcI
StyI TfiI Tru9I TseI Tsp509I
TspRI XcmI XhoII