##################################
# NO CUT ENZYMES ( 104 enzymes) #
##################################
Acc65I AclI AcsI AflII AgeI
AhdI Alw44I Aor51HI ApaI ApaLI
ApoI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AviII AvrII
BbrPI BcgI BfrI BglII BlnI
BsaAI BsaBI BsaI BsiWI BsmBI
BsmI Bsp1407I BspDI BspHI BsrBI
BsrDI BsrGI BssSI Bst1107I BstBI
BstEII BstPI BstZ17I ClaI DraI
DrdI DsaI Eam1105I Eco47III EcoNI
EcoO65I EcoRI EcoRV EcoT14I EcoT22I
FseI FspI HpaI KpnI KspI
MamI MfeI MluI MunI NaeI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PflFI
PinAI PmaCI PmeI PmlI PshBI
Psp1406I PstI PvuI RcaI SacII
SalI SbfI ScaI SexAI SfcI
SfiI SfuI SgrAI SnaBI SpeI
SphI Sse8387I SspBI SspI StyI
SwaI Tth111I XbaI XmnI
###################################
# ONE CUT ENZYMES ( 48 enzymes) #
###################################
AccIII 863 TCCGGA T^CCGGA
AfaI 606 GTAC GT^AC
AflIII 1670 ACATGT A^C[GA][CT]GT
AocI 1158 CCTGAGG CC^T[ACGT]AGG
BalI 653 TGGCCA TGG^CCA
BamHI 1154 GGATCC G^GATCC
BbeI 340 GGCGCC GGCGC^C
BbsI 406 GTCTTC GAAGAC(2/6)
BbvCI 273 GCTGAGG CCTCAGC(-5/-2)
BciVI 1186 GGATAC GTATCC(6/5)
BclI 929 TGATCA T^GATCA
BglI 88 GCCTGCGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 471 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 471 GCTCAGC GC^T[ACGT]AGC
BpuAI 406 GTCTTC GAAGAC(2/6)
BseAI 863 TCCGGA T^CCGGA
BsgI 667 CTGCAC GTGCAG(16/14)
BsmAI 1077 GTCTC GTCTC(1/5)
BspEI 863 TCCGGA T^CCGGA
BspLU11I 1670 ACATGT A^CATGT
BstAPI 241 GCAGGTGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 1554 CCAGTTTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 1158 CCTGAGG CC^T[ACGT]AGG
CelII 471 GCTCAGC GC^T[ACGT]AGC
CpoI 560 CGGACCG CG^G[AT]CCG
Eco81I 1158 CCTGAGG CC^T[ACGT]AGG
FbaI 929 TGATCA T^GATCA
HincII 589 GTCAAC GT[CT]^[GA]AC
HindII 589 GTCAAC GT[CT]^[GA]AC
HindIII 1366 AAGCTT A^AGCTT
KasI 340 GGCGCC G^GCGCC
MluNI 653 TGGCCA TGG^CCA
MroI 863 TCCGGA T^CCGGA
MscI 653 TGGCCA TGG^CCA
NarI 340 GGCGCC GG^CGCC
PaeR7I 756 CTCGAG C^TCGAG
PflMI 656 CCAGTCACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 1530 GACGTCCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RsaI 606 GTAC GT^AC
RsrII 560 CGGACCG CG^G[AT]CCG
SacI 793 GAGCTC GAGCT^C
SapI 795 GCTCTTC GCTCTTC(1/4)
SfoI 340 GGCGCC GGC^GCC
StuI 760 AGGCCT AGG^CCT
TfiI 729 GAATC G^A[AT]TC
Tsp509I 1 AATT ^AATT
Van91I 656 CCAGTCACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 756 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AatII 1138 GACGTC GACGT^C
1530 GACGTC
AccI 1577 GTCTAC GT^[AC][GT]AC
1666 GTCTAC
AspHI 793 GAGCTC G[AT]GC[AT]^C
1217 GTGCTC
BanII 793 GAGCTC G[GA]GC[CT]^C
996 GAGCCC
BsaWI 732 TCCGGT [AT]^CCGG[AT]
863 TCCGGA
BsiHKAI 793 GAGCTC G[AT]GC[AT]^C
1217 GTGCTC
BsrFI 171 GCCGGT [GA]^CCGG[CT]
1420 ACCGGC
BssHII 139 GCGCGC G^CGCGC
141 GCGCGC
BstYI 743 GGATCT [GA]^GATC[CT]
1154 GGATCC
Cfr10I 171 GCCGGT [GA]^CCGG[CT]
1420 ACCGGC
DraIII 573 CACCATGTG CAC[ACGT][ACGT][ACGT]^GTG
774 CACCTTGTG
EagI 251 CGGCCG C^GGCCG
935 CGGCCG
EarI 796 CTCTTC CTCTTC(1/4)
1745 CTCTTC
EclXI 251 CGGCCG C^GGCCG
935 CGGCCG
Eco52I 251 CGGCCG C^GGCCG
935 CGGCCG
HaeII 85 AGCGCC [GA]GCGC^[CT]
340 GGCGCC
Ksp632I 796 CTCTTC CTCTTC(1/4)
1745 CTCTTC
MflI 743 GGATCT [GA]^GATC[CT]
1154 GGATCC
MseI 518 TTAA T^TAA
1798 TTAA
MslI 1618 CATGCTCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1703 CACTAGAATG
NspI 551 ACATGC [GA]CATG^[CT]
1670 ACATGT
PvuII 49 CAGCTG CAG^CTG
70 CAGCTG
SmlI 756 CTCGAG C^T[CT][GA]AG
1292 CTTGAG
Tru9I 518 TTAA T^TAA
1798 TTAA
XhoII 743 GGATCT [GA]^GATC[CT]
1154 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 81 enzymes) #
#########################################
AccII AciI AcyI AluI AlwI
AlwNI AvaI AvaII BanI BbvI
BcnI BfaI BmyI BpmI BsaHI
BsaJI BseRI BsiEI BsiYI BslI
BsmFI BsoBI Bsp1286I BspMI BsrI
BssKI BstF5I BstNI BstUI Cac8I
CfoI Cfr13I DdeI DpnII DraII
EaeI EcoO109I EcoRII FauI Fnu4HI
FokI HaeIII HapII HgaI HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI MaeI MaeII MaeIII MboI
MboII MnlI MspA1I MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV PleI PpuMI Sau3AI Sau96I
ScrFI SfaNI SmaI TaqI TseI
Tsp45I TspRI TthHB8I XcmI XmaCI
XmaI