##################################
# NO CUT ENZYMES ( 98 enzymes) #
##################################
AatII AccI AclI AcyI Aor51HI
ApaI AscI AspI AvrII BamHI
BbeI BbrPI BbvCI BcgI BciVI
BlnI BsaBI BsaHI BsaI BsgI
BsiEI BsiWI BsiYI BslI Bsp1407I
BspDI BsrBI BsrGI BssHII BssSI
Bst1107I BstBI BstEII BstPI BstZ17I
ClaI CpoI DraIII DrdI EagI
EclXI Eco47III Eco52I EcoNI EcoO65I
EcoRI EcoRV FauI FseI HaeII
Hin1I HincII HindII HindIII HpaI
KasI KspI MamI MfeI MluI
MslI MunI NaeI NarI NdeI
NgoMIV NheI NotI NruI NspV
PacI PflFI PflMI PmaCI PmeI
PmlI PshAI Psp1406I PstI PvuI
RsrII SacII SalI SbfI SexAI
SfiI SfoI SfuI SgrAI SmaI
SpeI Sse8387I SspBI Tth111I Van91I
XbaI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 68 enzymes) #
###################################
Acc65I 724 GGTACC G^GTACC
AccIII 427 TCCGGA T^CCGGA
AflII 1129 CTTAAG C^TTAAG
AgeI 124 ACCGGT A^CCGGT
AhdI 1626 GACATGTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 2312 GTGCAC G^TGCAC
AlwI 81 GATCC GGATC(4/5)
AocI 1525 CCTTAGG CC^T[ACGT]AGG
ApaLI 2312 GTGCAC G^TGCAC
AseI 1157 ATTAAT AT^TAAT
AsnI 1157 ATTAAT AT^TAAT
Asp700I 2139 GAAAGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 724 GGTACC G^GTACC
AspEI 1626 GACATGTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI 1025 CTCGAG C^[CT]CG[GA]G
AviII 1267 TGCGCA TGC^GCA
BalI 1208 TGGCCA TGG^CCA
BanII 1511 GAGCTC G[GA]GC[CT]^C
BclI 1988 TGATCA T^GATCA
BfrI 1129 CTTAAG C^TTAAG
BglI 309 GCCCAATGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 830 AGATCT A^GATCT
BlpI 1508 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1508 GCTGAGC GC^T[ACGT]AGC
BseAI 427 TCCGGA T^CCGGA
BsmBI 875 GAGACG CGTCTC(1/5)
BsmFI 1530 GGGAC GGGAC(10/14)
BsmI 1790 GCATTC GAATGC(1/-1)
BsoBI 1025 CTCGAG C^[CT]CG[GA]G
BspEI 427 TCCGGA T^CCGGA
BspHI 1386 TCATGA T^CATGA
BsrFI 124 ACCGGT [GA]^CCGG[CT]
BsrI 1201 ACTGG ACTGG(1/-1)
BstF5I 941 CATCC GGATG(2/0)
BstXI 162 CCAGCAGAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 1525 CCTTAGG CC^T[ACGT]AGG
CelII 1508 GCTGAGC GC^T[ACGT]AGC
CfoI 1268 GCGC GCG^C
Cfr10I 124 ACCGGT [GA]^CCGG[CT]
EaeI 1208 TGGCCA [CT]^GGCC[GA]
Eam1105I 1626 GACATGTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 1525 CCTTAGG CC^T[ACGT]AGG
EcoT22I 1578 ATGCAT ATGCA^T
FbaI 1988 TGATCA T^GATCA
FokI 941 CATCC GGATG(9/13)
FspI 1267 TGCGCA TGC^GCA
HhaI 1268 GCGC GCG^C
HinP1I 1268 GCGC G^CGC
KpnI 724 GGTACC GGTAC^C
MluNI 1208 TGGCCA TGG^CCA
MroI 427 TCCGGA T^CCGGA
MscI 1208 TGGCCA TGG^CCA
NsiI 1578 ATGCAT ATGCA^T
PaeR7I 1025 CTCGAG C^TCGAG
PinAI 124 ACCGGT A^CCGGT
PpuMI 1133 AGGACCT [GA]G^G[AT]CC[CT]
PshBI 1157 ATTAAT AT^TAAT
RcaI 1386 TCATGA T^CATGA
SacI 1511 GAGCTC GAGCT^C
SapI 1808 GCTCTTC GCTCTTC(1/4)
ScaI 1964 AGTACT AGT^ACT
SnaBI 2081 TACGTA TAC^GTA
SphI 1576 GCATGC GCATG^C
StuI 526 AGGCCT AGG^CCT
SwaI 1168 ATTTAAAT ATTT^AAAT
XcmI 660 CCAAACTAGCAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 1025 CTCGAG C^TCGAG
XmnI 2139 GAAAGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AccII 20 CGCG CG^CG
41 CGCG
AflIII 1627 ACATGT A^C[GA][CT]GT
2196 ACATGT
AspHI 1511 GAGCTC G[AT]GC[AT]^C
2312 GTGCAC
AvaII 996 GGTCC G^G[AT]CC
1134 GGACC
BanI 642 GGTGCC G^G[CT][GA]CC
724 GGTACC
BbsI 396 GTCTTC GAAGAC(2/6)
1750 GTCTTC
BcnI 84 CCGGG CC^[GC]GG
784 CCGGG
BpuAI 396 GTCTTC GAAGAC(2/6)
1750 GTCTTC
BsaAI 51 TACGTG [CT]AC^GT[GA]
2081 TACGTA
BsaWI 124 ACCGGT [AT]^CCGG[AT]
427 TCCGGA
BsiHKAI 1511 GAGCTC G[AT]GC[AT]^C
2312 GTGCAC
BspLU11I 1627 ACATGT A^CATGT
2196 ACATGT
BspMI 621 ACCTGC ACCTGC(4/8)
1342 ACCTGC
BsrDI 2114 CATTGC GCAATG(2/0)
1598 GCAATG
BstAPI 262 GCAGCTCATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1821 GCAGTTGTTGC
BstUI 20 CGCG CG^CG
41 CGCG
BstYI 80 AGATCC [GA]^GATC[CT]
830 AGATCT
DraII 740 AGGGCCT [GA]G^G[ACGT]CC[CT]
1133 AGGACCT
DsaI 140 CCATGG C^C[GA][CT]GG
333 CCATGG
EcoO109I 740 AGGGCCT [GA]G^G[ACGT]CC[CT]
1133 AGGACCT
HgaI 18 GACGC GACGC(5/10)
39 GACGC
MflI 80 AGATCC [GA]^GATC[CT]
830 AGATCT
MvnI 20 CGCG CG^CG
41 CGCG
NciI 84 CCGGG CC^[GC]GG
784 CCGGG
NcoI 140 CCATGG C^CATGG
333 CCATGG
PleI 338 GAGTC GAGTC(4/5)
472 GAGTC
TaqI 1026 TCGA T^CGA
2566 TCGA
TthHB8I 1026 TCGA T^CGA
2566 TCGA
XhoII 80 AGATCC [GA]^GATC[CT]
830 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 63 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwNI
ApoI BbvI BfaI BmyI BpmI
BsaJI BseRI BsmAI Bsp1286I BssKI
BstNI Cac8I Cfr13I DdeI DpnII
DraI EarI EcoRII EcoT14I Fnu4HI
HaeIII HapII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MnlI MseI MspA1I
MspI MvaI MwoI NdeII NlaIII
NlaIV NspI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
SspI StyI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI