##################################
# NO CUT ENZYMES ( 100 enzymes) #
##################################
AatII Acc65I AccI AclI AflII
AflIII AgeI AhdI AscI Asp700I
Asp718I AspEI AspI AviII BamHI
BbeI BbrPI BcgI BclI BfrI
BglI BglII BsaAI BsaBI BsiWI
BsmBI Bsp1407I BspDI BspHI BspLU11I
BspMI BsrDI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstZ17I ClaI
CpoI DraI DraIII Eam1105I EarI
EcoRI EcoRV FbaI FseI FspI
HindIII HinfI HpaI KasI KpnI
Ksp632I KspI MamI MfeI MluI
MunI NarI NcoI NheI NotI
NruI NspV PacI PaeR7I PflFI
PflMI PinAI PleI PmaCI PmeI
PmlI Psp1406I PvuI RcaI RsrII
SacII SalI SapI SbfI SexAI
SfiI SfoI SfuI SgrAI SnaBI
SpeI Sse8387I SspBI SspI SwaI
TfiI Tth111I Van91I XhoI XmnI
###################################
# ONE CUT ENZYMES ( 50 enzymes) #
###################################
AccIII 121 TCCGGA T^CCGGA
AcyI 968 GGCGTC G[GA]^CG[CT]C
Alw44I 1025 GTGCAC G^TGCAC
AlwNI 288 CAGCTCCTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 202 AGCGCT AGC^GCT
ApaLI 1025 GTGCAC G^TGCAC
AseI 1508 ATTAAT AT^TAAT
AsnI 1508 ATTAAT AT^TAAT
AvaI 799 CCCGGG C^[CT]CG[GA]G
BanI 636 GGTGCC G^G[CT][GA]CC
BbsI 219 GTCTTC GAAGAC(2/6)
BbvCI 1410 CCTCAGC CCTCAGC(-5/-2)
BciVI 1153 GGATAC GTATCC(6/5)
BpuAI 219 GTCTTC GAAGAC(2/6)
BsaHI 968 GGCGTC G[GA]^CG[CT]C
BsaI 333 GAGACC GGTCTC(1/5)
BsaWI 121 TCCGGA [AT]^CCGG[AT]
BseAI 121 TCCGGA T^CCGGA
BsiEI 47 CGGCCG CG[GA][CT]^CG
BsmI 1167 GCATTC GAATGC(1/-1)
BsoBI 799 CCCGGG C^[CT]CG[GA]G
BspEI 121 TCCGGA T^CCGGA
BsrBI 763 CCGCTC CCGCTC(-3/-3)
BstXI 1132 CCAGGCTGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DrdI 375 GACCACGCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 601 CCACGG C^C[GA][CT]GG
EagI 47 CGGCCG C^GGCCG
EclXI 47 CGGCCG C^GGCCG
Eco47III 202 AGCGCT AGC^GCT
Eco52I 47 CGGCCG C^GGCCG
EcoT22I 1300 ATGCAT ATGCA^T
Hin1I 968 GGCGTC G[GA]^CG[CT]C
HincII 1768 GTTGAC GT[CT]^[GA]AC
HindII 1768 GTTGAC GT[CT]^[GA]AC
HphI 174 TCACC GGTGA(8/7)
MaeII 1538 ACGT A^CGT
MboII 220 TCTTC GAAGA(8/7)
MroI 121 TCCGGA T^CCGGA
NaeI 45 GCCGGC GCC^GGC
NdeI 1314 CATATG CA^TATG
NgoMIV 45 GCCGGC G^CCGGC
NsiI 1300 ATGCAT ATGCA^T
PshBI 1508 ATTAAT AT^TAAT
PvuII 1382 CAGCTG CAG^CTG
SacI 660 GAGCTC GAGCT^C
ScaI 1348 AGTACT AGT^ACT
SmaI 799 CCCGGG CCC^GGG
SmlI 432 CTCAAG C^T[CT][GA]AG
XmaCI 799 CCCGGG C^CCGGG
XmaI 799 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 36 enzymes) #
###################################
AccII 78 CGCG CG^CG
330 CGCG
AcsI 1293 AAATTT [GA]^AATT[CT]
1429 AAATTC
AfaI 581 GTAC GT^AC
1349 GTAC
AocI 752 CCTCAGG CC^T[ACGT]AGG
1784 CCTAAGG
ApaI 819 GGGCCC GGGCC^C
1035 GGGCCC
ApoI 1293 AAATTT [GA]^AATT[CT]
1429 AAATTC
AvrII 422 CCTAGG C^CTAGG
1404 CCTAGG
BlnI 422 CCTAGG C^CTAGG
1404 CCTAGG
BlpI 1453 GCTCAGC GC^T[ACGT]AGC
1734 GCTGAGC
Bpu1102I 1453 GCTCAGC GC^T[ACGT]AGC
1734 GCTGAGC
BsgI 483 CTGCAC GTGCAG(16/14)
736 CTGCAC
BsrFI 45 GCCGGC [GA]^CCGG[CT]
487 ACCGGC
BstEII 172 GGTCACC G^GT[ACGT]ACC
1571 GGTAACC
BstPI 172 GGTCACC G^GT[ACGT]ACC
1571 GGTAACC
BstUI 78 CGCG CG^CG
330 CGCG
BstYI 998 GGATCT [GA]^GATC[CT]
1618 GGATCT
Bsu36I 752 CCTCAGG CC^T[ACGT]AGG
1784 CCTAAGG
CelII 1453 GCTCAGC GC^T[ACGT]AGC
1734 GCTGAGC
Cfr10I 45 GCCGGC [GA]^CCGG[CT]
487 ACCGGC
Eco81I 752 CCTCAGG CC^T[ACGT]AGG
1784 CCTAAGG
EcoNI 1237 CCTTCTAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1694 CCTCCAGCAGG
EcoO65I 172 GGTCACC G^GT[ACGT]ACC
1571 GGTAACC
HgaI 262 GCGTC GACGC(5/10)
969 GCGTC
MflI 998 GGATCT [GA]^GATC[CT]
1618 GGATCT
MslI 692 CACTGAAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1056 CATTTCGGTG
MspA1I 913 CCGCTG C[AC]G^C[GT]G
1382 CAGCTG
MvnI 78 CGCG CG^CG
330 CGCG
NspI 307 GCATGC [GA]CATG^[CT]
530 GCATGC
PshAI 149 GACCCCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
1771 GACACAGGTC
PstI 14 CTGCAG CTGCA^G
619 CTGCAG
RsaI 581 GTAC GT^AC
1349 GTAC
SphI 307 GCATGC GCATG^C
530 GCATGC
TaqI 352 TCGA T^CGA
730 TCGA
TthHB8I 352 TCGA T^CGA
730 TCGA
XbaI 1331 TCTAGA T^CTAGA
1746 TCTAGA
XhoII 998 GGATCT [GA]^GATC[CT]
1618 GGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 72 enzymes) #
#########################################
AciI AluI AlwI AspHI AvaII
BalI BanII BbvI BcnI BfaI
BmyI BpmI BsaJI BseRI BsiHKAI
BsiYI BslI BsmAI BsmFI Bsp1286I
BsrI BssKI BstF5I BstNI Cac8I
CfoI Cfr13I DdeI DpnII DraII
EaeI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HhaI HinP1I HpaII ItaI MaeI
MaeIII MboI MluNI MnlI MscI
MseI MspI MvaI MwoI NciI
NdeII NlaIII NlaIV PpuMI Sau3AI
Sau96I ScrFI SfaNI SfcI StuI
StyI Tru9I TseI Tsp45I Tsp509I
TspRI XcmI