##################################
# NO CUT ENZYMES ( 120 enzymes) #
##################################
AccI AccII AccIII AclI AflII
AflIII AgeI AhdI Alw44I ApaI
ApaLI AscI AseI AsnI AspEI
AspI AviII AvrII BbeI BbrPI
BcgI BciVI BclI BcnI BfrI
BlnI BlpI Bpu1102I BsaAI BsaBI
BsaWI BseAI BsiEI BsiWI BsmBI
BspDI BspEI BspHI BspLU11I BsrBI
BssHII BssSI Bst1107I BstAPI BstBI
BstUI BstZ17I CelII ClaI CpoI
DraI DraIII DrdI EagI Eam1105I
EclXI Eco52I EcoRV EcoT22I FbaI
FseI FspI HincII HindII HindIII
HpaI KasI KspI MamI MfeI
MluI MroI MunI MvnI NarI
NciI NdeI NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
RcaI RsrII SacII SalI SapI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI SwaI Tth111I
Van91I XbaI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 52 enzymes) #
###################################
AatII 33 GACGTC GACGT^C
Acc65I 1121 GGTACC G^GTACC
AcsI 1296 GAATTC [GA]^AATT[CT]
Aor51HI 1575 AGCGCT AGC^GCT
ApoI 1296 GAATTC [GA]^AATT[CT]
Asp718I 1121 GGTACC G^GTACC
AspHI 825 GAGCTC G[AT]GC[AT]^C
AvaI 594 CTCGGG C^[CT]CG[GA]G
BamHI 143 GGATCC G^GATCC
BanI 1121 GGTACC G^G[CT][GA]CC
BbvCI 1591 CCTCAGC CCTCAGC(-5/-2)
BglI 42 GCCCAGATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsgI 1464 GTGCAG GTGCAG(16/14)
BsiHKAI 825 GAGCTC G[AT]GC[AT]^C
BsmI 758 GAATGC GAATGC(1/-1)
BsoBI 594 CTCGGG C^[CT]CG[GA]G
Bsp1407I 775 TGTACA T^GTACA
BsrDI 522 GCAATG GCAATG(2/0)
BsrFI 871 GCCGGC [GA]^CCGG[CT]
BsrGI 775 TGTACA T^GTACA
BstEII 8 GGTGACC G^GT[ACGT]ACC
BstPI 8 GGTGACC G^GT[ACGT]ACC
CfoI 1576 GCGC GCG^C
Cfr10I 871 GCCGGC [GA]^CCGG[CT]
DsaI 1726 CCATGG C^C[GA][CT]GG
EarI 1479 CTCTTC CTCTTC(1/4)
Eco47III 1575 AGCGCT AGC^GCT
EcoO65I 8 GGTGACC G^GT[ACGT]ACC
EcoRI 1296 GAATTC G^AATTC
HaeII 1575 AGCGCT [GA]GCGC^[CT]
HapII 872 CCGG C^CGG
HgaI 813 GACGC GACGC(5/10)
HhaI 1576 GCGC GCG^C
HinP1I 1576 GCGC G^CGC
HpaII 872 CCGG C^CGG
KpnI 1121 GGTACC GGTAC^C
Ksp632I 1479 CTCTTC CTCTTC(1/4)
MaeII 34 ACGT A^CGT
MseI 1321 TTAA T^TAA
MspI 872 CCGG C^CGG
NaeI 871 GCCGGC GCC^GGC
NcoI 1726 CCATGG C^CATGG
NgoMIV 871 GCCGGC G^CCGGC
PvuII 1083 CAGCTG CAG^CTG
SacI 825 GAGCTC GAGCT^C
SbfI 1553 CCTGCAGG CCTGCA^GG
Sse8387I 1553 CCTGCAGG CCTGCA^GG
SspBI 775 TGTACA T^GTACA
TaqI 1303 TCGA T^CGA
TfiI 381 GATTC G^A[AT]TC
Tru9I 1321 TTAA T^TAA
TthHB8I 1303 TCGA T^CGA
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AcyI 33 GACGTC G[GA]^CG[CT]C
813 GACGCC
AocI 1652 CCTCAGG CC^T[ACGT]AGG
1684 CCTGAGG
Asp700I 677 GAAGGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
978 GAACCCTTTC
BalI 736 TGGCCA TGG^CCA
844 TGGCCA
BfaI 879 CTAG C^TAG
1770 CTAG
BglII 715 AGATCT A^GATCT
1349 AGATCT
BsaHI 33 GACGTC G[GA]^CG[CT]C
813 GACGCC
BsaI 537 GAGACC GGTCTC(1/5)
1549 GAGACC
BseRI 107 CTCCTC GAGGAG(10/8)
912 CTCCTC
BspMI 1552 ACCTGC ACCTGC(4/8)
1704 ACCTGC
BstXI 13 CCAGAAGTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1429 CCATCTCTGTGG
Bsu36I 1652 CCTCAGG CC^T[ACGT]AGG
1684 CCTGAGG
EaeI 736 TGGCCA [CT]^GGCC[GA]
844 TGGCCA
Eco81I 1652 CCTCAGG CC^T[ACGT]AGG
1684 CCTGAGG
EcoNI 578 CCTTACCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1648 CCTGCCTCAGG
Hin1I 33 GACGTC G[GA]^CG[CT]C
813 GACGCC
MaeI 879 CTAG C^TAG
1770 CTAG
MluNI 736 TGGCCA TGG^CCA
844 TGGCCA
MscI 736 TGGCCA TGG^CCA
844 TGGCCA
MspA1I 28 CCGCTG C[AC]G^C[GT]G
1083 CAGCTG
XcmI 392 CCAGCCCACTACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
613 CCAAAACAAACCTGG
XmnI 677 GAAGGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
978 GAACCCTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 64 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
AvaII BanII BbsI BbvI BmyI
BpmI BpuAI BsaJI BsiYI BslI
BsmAI BsmFI Bsp1286I BsrI BssKI
BstF5I BstNI BstYI Cac8I Cfr13I
DdeI DpnII DraII EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeIII
HinfI HphI ItaI MaeIII MboI
MboII MflI MnlI MslI MvaI
MwoI NdeII NlaIII NlaIV PleI
PpuMI PstI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI StyI TseI
Tsp45I Tsp509I TspRI XhoII