##################################
# NO CUT ENZYMES ( 113 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcyI AgeI AhdI Alw44I AocI
ApaLI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AviII BamHI
BbeI BbrPI BcgI BclI BglII
BsaHI BsaI BsaWI BseAI BsiWI
Bsp1407I BspDI BspEI BsrBI BsrFI
BsrGI BssHII BssSI Bst1107I BstBI
BstEII BstPI BstUI BstYI BstZ17I
Bsu36I Cfr10I ClaI CpoI DraI
EagI Eam1105I EclXI Eco52I Eco81I
EcoO65I EcoRV FbaI FseI FspI
Hin1I HpaI KasI KpnI KspI
MfeI MflI MluI MroI MslI
MunI MvnI NaeI NarI NcoI
NgoMIV NheI NotI NruI NspV
PacI PflFI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
RsrII SacII SalI ScaI SexAI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI SspBI SspI StuI
SwaI Tth111I XbaI XcmI XhoII
XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 56 enzymes) #
###################################
AccI 267 GTCTAC GT^[AC][GT]AC
AcsI 1447 GAATTC [GA]^AATT[CT]
AflII 1236 CTTAAG C^TTAAG
AlwI 1363 GATCC GGATC(4/5)
Aor51HI 224 AGCGCT AGC^GCT
ApaI 1420 GGGCCC GGGCC^C
ApoI 1447 GAATTC [GA]^AATT[CT]
AspHI 1241 GAGCTC G[AT]GC[AT]^C
AvrII 1078 CCTAGG C^CTAGG
BanI 891 GGCACC G^G[CT][GA]CC
BbsI 534 GAAGAC GAAGAC(2/6)
BbvCI 872 GCTGAGG CCTCAGC(-5/-2)
BciVI 478 GTATCC GTATCC(6/5)
BcnI 394 CCCGG CC^[GC]GG
BfrI 1236 CTTAAG C^TTAAG
BlnI 1078 CCTAGG C^CTAGG
BlpI 1243 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 1243 GCTCAGC GC^T[ACGT]AGC
BpuAI 534 GAAGAC GAAGAC(2/6)
BsaAI 270 TACGTG [CT]AC^GT[GA]
BsaBI 1653 GATGGCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsgI 142 GTGCAG GTGCAG(16/14)
BsiEI 64 CGGTCG CG[GA][CT]^CG
BsiHKAI 1241 GAGCTC G[AT]GC[AT]^C
BsmAI 105 GTCTC GTCTC(1/5)
BsmBI 104 CGTCTC CGTCTC(1/5)
BsmI 1555 GAATGC GAATGC(1/-1)
BspHI 1085 TCATGA T^CATGA
BspMI 719 GCAGGT ACCTGC(4/8)
BstAPI 188 GCAATTTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 101 CCACGTCTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 1243 GCTCAGC GC^T[ACGT]AGC
DraIII 136 CACTCAGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 40 GACGAAGCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 61 CCACGG C^C[GA][CT]GG
EarI 1409 GAAGAG CTCTTC(1/4)
Eco47III 224 AGCGCT AGC^GCT
EcoRI 1447 GAATTC G^AATTC
EcoT22I 1744 ATGCAT ATGCA^T
HgaI 1767 GACGC GACGC(5/10)
HincII 609 GTTGAC GT[CT]^[GA]AC
HindII 609 GTTGAC GT[CT]^[GA]AC
Ksp632I 1409 GAAGAG CTCTTC(1/4)
MamI 1653 GATGGCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NciI 394 CCCGG CC^[GC]GG
NdeI 484 CATATG CA^TATG
NsiI 1744 ATGCAT ATGCA^T
PaeR7I 603 CTCGAG C^TCGAG
PstI 669 CTGCAG CTGCA^G
RcaI 1085 TCATGA T^CATGA
SacI 1241 GAGCTC GAGCT^C
SapI 1409 GAAGAGC GCTCTTC(1/4)
SbfI 668 CCTGCAGG CCTGCA^GG
SphI 1355 GCATGC GCATG^C
Sse8387I 668 CCTGCAGG CCTGCA^GG
XhoI 603 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AfaI 36 GTAC GT^AC
1168 GTAC
AvaI 603 CTCGAG C^[CT]CG[GA]G
1641 CTCGGG
BglI 204 GCCAAGTCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
213 GCCAAGGGGGC
BseRI 714 GAGGAG GAGGAG(10/8)
1137 GAGGAG
BsoBI 603 CTCGAG C^[CT]CG[GA]G
1641 CTCGGG
BsrDI 656 CATTGC GCAATG(2/0)
1200 CATTGC
DpnII 1119 GATC ^GATC
1363 GATC
EcoNI 25 CCTGACCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1126 CCTTCTCTAGG
HapII 341 CCGG C^CGG
395 CCGG
HindIII 432 AAGCTT A^AGCTT
1647 AAGCTT
HpaII 341 CCGG C^CGG
395 CCGG
MboI 1119 GATC ^GATC
1363 GATC
MspI 341 CCGG C^CGG
395 CCGG
NdeII 1119 GATC ^GATC
1363 GATC
PflMI 882 CCAGCTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
952 CCAAGATTTGG
RsaI 36 GTAC GT^AC
1168 GTAC
Sau3AI 1119 GATC ^GATC
1363 GATC
SfaNI 619 GCATC GCATC(5/9)
1248 GCATC
SmlI 603 CTCGAG C^T[CT][GA]AG
1236 CTTAAG
Van91I 882 CCAGCTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
952 CCAAGATTTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 69 enzymes) #
#########################################
AciI AflIII AluI AlwNI AvaII
BalI BanII BbvI BfaI BmyI
BpmI BsaJI BsiYI BslI BsmFI
Bsp1286I BspLU11I BsrI BssKI BstF5I
BstNI Cac8I CfoI Cfr13I DdeI
DraII EaeI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HhaI HinP1I HinfI HphI ItaI
MaeI MaeII MaeIII MboII MluNI
MnlI MscI MseI MspA1I MvaI
MwoI NlaIII NlaIV NspI PleI
PpuMI PvuII Sau96I ScrFI SfcI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I