##################################
# NO CUT ENZYMES ( 114 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcyI AflII AgeI AhdI AlwNI
ApaI AscI Asp718I AspEI AspI
AvaI AviII AvrII BamHI BbeI
BbvCI BcgI BclI BfrI BglI
BglII BlnI BlpI Bpu1102I BsaBI
BsaHI BsaWI BseAI BsgI BsiEI
BsiWI BsmBI BsoBI Bsp1407I BspEI
BsrBI BsrFI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstZ17I CelII Cfr10I CpoI DraIII
DrdI EagI Eam1105I EclXI Eco52I
EcoNI EcoO65I EcoRI EcoRV EcoT22I
FbaI FseI FspI HgaI Hin1I
HindIII HpaI KasI KpnI KspI
MamI MluI MroI NaeI NarI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PinAI PmeI PshAI Psp1406I PvuI
RsrII SacII SalI SbfI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SphI Sse8387I SspBI SwaI Tth111I
XbaI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 50 enzymes) #
###################################
AflIII 2061 ACATGT A^C[GA][CT]GT
Alw44I 53 GTGCAC G^TGCAC
AocI 1021 CCTCAGG CC^T[ACGT]AGG
Aor51HI 154 AGCGCT AGC^GCT
ApaLI 53 GTGCAC G^TGCAC
AseI 1134 ATTAAT AT^TAAT
AsnI 1134 ATTAAT AT^TAAT
Asp700I 1598 GAAGCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BalI 1816 TGGCCA TGG^CCA
BanI 1470 GGTGCC G^G[CT][GA]CC
BanII 207 GAGCTC G[GA]GC[CT]^C
BbrPI 2130 CACGTG CAC^GTG
BfaI 823 CTAG C^TAG
BsaI 462 GGTCTC GGTCTC(1/5)
BsmI 1957 GCATTC GAATGC(1/-1)
BspDI 2097 ATCGAT AT^CGAT
BspLU11I 2061 ACATGT A^CATGT
BsrDI 1922 CATTGC GCAATG(2/0)
Bsu36I 1021 CCTCAGG CC^T[ACGT]AGG
ClaI 2097 ATCGAT AT^CGAT
DraI 1179 TTTAAA TTT^AAA
DraII 479 GGGACCC [GA]G^G[ACGT]CC[CT]
EaeI 1816 TGGCCA [CT]^GGCC[GA]
Eco47III 154 AGCGCT AGC^GCT
Eco81I 1021 CCTCAGG CC^T[ACGT]AGG
EcoO109I 479 GGGACCC [GA]G^G[ACGT]CC[CT]
FauI 303 CCCGC CCCGC(4/6)
HaeII 154 AGCGCT [GA]GCGC^[CT]
HincII 1251 GTCAAC GT[CT]^[GA]AC
HindII 1251 GTCAAC GT[CT]^[GA]AC
MaeI 823 CTAG C^TAG
MfeI 2167 CAATTG C^AATTG
MluNI 1816 TGGCCA TGG^CCA
MscI 1816 TGGCCA TGG^CCA
MunI 2167 CAATTG C^AATTG
NcoI 1807 CCATGG C^CATGG
PflMI 1962 CCACGATATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 2130 CACGTG CAC^GTG
PmlI 2130 CACGTG CAC^GTG
PpuMI 479 GGGACCC [GA]G^G[AT]CC[CT]
PshBI 1134 ATTAAT AT^TAAT
PstI 1111 CTGCAG CTGCA^G
PvuII 1078 CAGCTG CAG^CTG
SacI 207 GAGCTC GAGCT^C
ScaI 1948 AGTACT AGT^ACT
SexAI 458 ACCTGGT A^CC[AT]GGT
SmlI 893 CTCAAG C^T[CT][GA]AG
SpeI 822 ACTAGT A^CTAGT
Van91I 1962 CCACGATATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI 1598 GAAGCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AcsI 1995 GAATTT [GA]^AATT[CT]
2209 AAATTT
ApoI 1995 GAATTT [GA]^AATT[CT]
2209 AAATTT
AvaII 111 GGTCC G^G[AT]CC
480 GGACC
BciVI 1065 GGATAC GTATCC(6/5)
1626 GGATAC
BcnI 114 CCGGG CC^[GC]GG
1300 CCGGG
BsaAI 609 TACGTG [CT]AC^GT[GA]
2130 CACGTG
BseRI 1256 CTCCTC GAGGAG(10/8)
805 GAGGAG
BsmAI 1272 GAGAC GTCTC(1/5)
463 GTCTC
BspMI 581 ACCTGC ACCTGC(4/8)
1827 GCAGGT
BstYI 182 AGATCC [GA]^GATC[CT]
533 AGATCC
CfoI 155 GCGC GCG^C
168 GCGC
HapII 114 CCGG C^CGG
1300 CCGG
HhaI 155 GCGC GCG^C
168 GCGC
HinP1I 155 GCGC G^CGC
168 GCGC
HpaII 114 CCGG C^CGG
1300 CCGG
MaeII 610 ACGT A^CGT
2131 ACGT
MflI 182 AGATCC [GA]^GATC[CT]
533 AGATCC
MslI 1148 CACTGCTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1682 CATGAGGATG
MspA1I 1078 CAGCTG C[AC]G^C[GT]G
1622 CAGCGG
MspI 114 CCGG C^CGG
1300 CCGG
NciI 114 CCGGG CC^[GC]GG
1300 CCGGG
NspI 57 ACATGC [GA]CATG^[CT]
2061 ACATGT
SapI 675 GCTCTTC GCTCTTC(1/4)
1601 GCTCTTC
XcmI 856 CCATCTTGTTCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1511 CCAGAACAGAGCTGG
XhoII 182 AGATCC [GA]^GATC[CT]
533 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 69 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AspHI BbsI BbvI BmyI BpmI
BpuAI BsaJI BsiHKAI BsiYI BslI
BsmFI Bsp1286I BspHI BsrI BssKI
BstF5I BstNI BstUI BstXI Cac8I
Cfr13I DdeI DpnII DsaI EarI
EcoRII EcoT14I Fnu4HI FokI HaeIII
HinfI HphI ItaI Ksp632I MaeIII
MboI MboII MnlI MseI MvaI
MvnI MwoI NdeII NlaIII NlaIV
PleI RcaI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SspI StuI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I