##################################
# NO CUT ENZYMES ( 122 enzymes) #
##################################
AatII Acc65I AccIII AclI AcyI
AflII AgeI Alw44I AlwNI AocI
ApaI ApaLI AscI AseI AsnI
Asp718I AvaI AviII BalI BamHI
BanI BanII BbeI BbrPI BbvCI
BcgI BciVI BclI BcnI BfrI
BglI BlpI Bpu1102I BsaAI BsaBI
BsaHI BsaWI BseAI BsiWI BsoBI
BspEI BspHI BsrBI BssHII BssSI
Bst1107I BstBI BstXI BstZ17I Bsu36I
CelII CpoI DraII DraIII DrdI
DsaI EaeI EagI EclXI Eco52I
Eco81I EcoNI EcoO109I EcoRI EcoRV
FbaI FseI FspI HaeIII Hin1I
HpaI KasI KpnI KspI MamI
MfeI MluI MluNI MroI MscI
MunI NarI NciI NcoI NheI
NotI NruI NspV PacI PaeR7I
PinAI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PstI RcaI
RsrII SacI SacII SapI SbfI
SexAI SfcI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I StuI SwaI XbaI XhoI
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 56 enzymes) #
###################################
AccII 9 CGCG CG^CG
AflIII 646 ACATGT A^C[GA][CT]GT
AhdI 65 GACTTGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 213 AGCGCT AGC^GCT
Asp700I 1262 GAAAGGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 65 GACTTGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 1125 GAGCAC G[AT]GC[AT]^C
AspI 12 GACGCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 1147 CCTAGG C^CTAGG
BbsI 397 GTCTTC GAAGAC(2/6)
BfaI 1148 CTAG C^TAG
BlnI 1147 CCTAGG C^CTAGG
BpmI 1174 CTGGAG CTGGAG(16/14)
BpuAI 397 GTCTTC GAAGAC(2/6)
BsaI 360 GAGACC GGTCTC(1/5)
BsgI 977 GTGCAG GTGCAG(16/14)
BsiEI 1330 CGATCG CG[GA][CT]^CG
BsiHKAI 1125 GAGCAC G[AT]GC[AT]^C
BsmI 1188 GCATTC GAATGC(1/-1)
Bsp1407I 1618 TGTACA T^GTACA
BspLU11I 646 ACATGT A^CATGT
BspMI 264 GCAGGT ACCTGC(4/8)
BsrDI 1253 GCAATG GCAATG(2/0)
BsrGI 1618 TGTACA T^GTACA
BstAPI 1179 GCATAGGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 527 GGTCACC G^GT[ACGT]ACC
BstPI 527 GGTCACC G^GT[ACGT]ACC
BstUI 9 CGCG CG^CG
DraI 1599 TTTAAA TTT^AAA
Eam1105I 65 GACTTGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 213 AGCGCT AGC^GCT
EcoO65I 527 GGTCACC G^GT[ACGT]ACC
EcoT14I 1147 CCTAGG C^C[AT][AT]GG
EcoT22I 1186 ATGCAT ATGCA^T
FauI 635 GCGGG CCCGC(4/6)
HaeII 213 AGCGCT [GA]GCGC^[CT]
HindIII 49 AAGCTT A^AGCTT
MaeI 1148 CTAG C^TAG
MslI 191 CATAGCAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI 9 CGCG CG^CG
NaeI 1361 GCCGGC GCC^GGC
NgoMIV 1361 GCCGGC G^CCGGC
NlaIV 1155 GGTCCC GG[ACGT]^[ACGT]CC
NsiI 1186 ATGCAT ATGCA^T
NspI 646 ACATGT [GA]CATG^[CT]
PflFI 12 GACGCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PvuI 1330 CGATCG CGAT^CG
PvuII 20 CAGCTG CAG^CTG
SalI 294 GTCGAC G^TCGAC
SspBI 1618 TGTACA T^GTACA
SspI 1317 AATATT AAT^ATT
StyI 1147 CCTAGG C^C[AT][AT]GG
TspRI 196 CAGTG CA[GC]TG[ACGT][ACGT]^
Tth111I 12 GACGCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 1163 CCATGTGATGTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 1262 GAAAGGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 35 enzymes) #
###################################
AciI 754 CCGC CCGC(-3/-1)
635 GCGG
AlwI 1469 GATCC GGATC(4/5)
874 GGATC
AvaII 1155 GGTCC G^G[AT]CC
1274 GGACC
BglII 1542 AGATCT A^GATCT
1571 AGATCT
BmyI 198 GTGCCC G[AGT]GC[ACT]^C
1125 GAGCAC
BsaJI 201 CCCAGG C^C[ACGT][ACGT]GG
1147 CCTAGG
BseRI 154 GAGGAG GAGGAG(10/8)
521 GAGGAG
BsmBI 1433 CGTCTC CGTCTC(1/5)
549 GAGACG
BsmFI 820 GGGAC GGGAC(10/14)
1156 GTCCC
Bsp1286I 198 GTGCCC G[AGT]GC[ACT]^C
1125 GAGCAC
BspDI 623 ATCGAT AT^CGAT
1332 ATCGAT
BsrFI 372 GCCGGT [GA]^CCGG[CT]
1361 GCCGGC
BsrI 871 ACTGG ACTGG(1/-1)
1341 ACTGG
BstYI 1542 AGATCT [GA]^GATC[CT]
1571 AGATCT
Cac8I 695 GCTCGC GC[ACGT]^[ACGT]GC
1361 GCCGGC
CfoI 8 GCGC GCG^C
214 GCGC
Cfr10I 372 GCCGGT [GA]^CCGG[CT]
1361 GCCGGC
Cfr13I 1155 GGTCC G^G[ACGT]CC
1274 GGACC
ClaI 623 ATCGAT AT^CGAT
1332 ATCGAT
HapII 373 CCGG C^CGG
1362 CCGG
HhaI 8 GCGC GCG^C
214 GCGC
HinP1I 8 GCGC G^CGC
214 GCGC
HincII 294 GTCGAC GT[CT]^[GA]AC
937 GTTGAC
HindII 294 GTCGAC GT[CT]^[GA]AC
937 GTTGAC
HpaII 373 CCGG C^CGG
1362 CCGG
MflI 1542 AGATCT [GA]^GATC[CT]
1571 AGATCT
MspA1I 20 CAGCTG C[AC]G^C[GT]G
754 CCGCTG
MspI 373 CCGG C^CGG
1362 CCGG
MwoI 42 GCGTTACAAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
1179 GCATAGGATGC
NdeI 1077 CATATG CA^TATG
1289 CATATG
PflMI 250 CCATTATTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
763 CCAGATGTTGG
Sau96I 1155 GGTCC G^G[ACGT]CC
1274 GGACC
SmlI 330 CTCAAG C^T[CT][GA]AG
1413 CTCAAG
Van91I 250 CCATTATTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
763 CCAGATGTTGG
XhoII 1542 AGATCT [GA]^GATC[CT]
1571 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 45 enzymes) #
#########################################
AccI AcsI AfaI AluI ApoI
BbvI BsiYI BslI BsmAI BssKI
BstF5I BstNI DdeI DpnII EarI
EcoRII Fnu4HI FokI HgaI HinfI
HphI ItaI Ksp632I MaeII MaeIII
MboI MboII MnlI MseI MvaI
NdeII NlaIII PleI RsaI Sau3AI
ScaI ScrFI SfaNI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TthHB8I