##################################
# NO CUT ENZYMES ( 110 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AflII AflIII AgeI AlwNI
AocI ApaI ApoI AscI AseI
AsnI Asp700I Asp718I BamHI BbeI
BbrPI BbvCI BciVI BclI BfrI
BlpI Bpu1102I BsaAI BsaBI BsaWI
BseAI BsiEI BsiWI BsmBI BsmI
BspDI BspEI BspLU11I BsrBI BsrDI
BssHII Bst1107I BstAPI BstBI BstZ17I
Bsu36I CelII ClaI CpoI DraI
DrdI EagI EclXI Eco52I Eco81I
EcoRI EcoRV EcoT22I FbaI FseI
HindIII HpaI KasI KpnI KspI
MamI MfeI MluI MroI MseI
MunI NarI NcoI NdeI NheI
NotI NruI NsiI NspI NspV
PacI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI RsrII
SacII SalI SapI SbfI ScaI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspI
SwaI Tru9I XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 52 enzymes) #
###################################
AccII 142 CGCG CG^CG
AcyI 1195 GGCGTC G[GA]^CG[CT]C
AhdI 877 GACTTCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 1263 GTGCAC G^TGCAC
Aor51HI 1304 AGCGCT AGC^GCT
ApaLI 1263 GTGCAC G^TGCAC
AspEI 877 GACTTCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 1149 GACCGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 1476 CTCGAG C^[CT]CG[GA]G
AviII 644 TGCGCA TGC^GCA
AvrII 65 CCTAGG C^CTAGG
BbsI 780 GAAGAC GAAGAC(2/6)
BcgI 1416 GCAAGCCCTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglI 1584 GCCCTGTTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 65 CCTAGG C^CTAGG
BpuAI 780 GAAGAC GAAGAC(2/6)
BsaHI 1195 GGCGTC G[GA]^CG[CT]C
BsoBI 1476 CTCGAG C^[CT]CG[GA]G
Bsp1407I 935 TGTACA T^GTACA
BspHI 602 TCATGA T^CATGA
BspMI 902 GCAGGT ACCTGC(4/8)
BsrGI 935 TGTACA T^GTACA
BstEII 1427 GGTCACC G^GT[ACGT]ACC
BstPI 1427 GGTCACC G^GT[ACGT]ACC
BstUI 142 CGCG CG^CG
DraIII 1654 CACCAAGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 1530 CCGTGG C^C[GA][CT]GG
Eam1105I 877 GACTTCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 1304 AGCGCT AGC^GCT
EcoNI 60 CCTCTCCTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 1427 GGTCACC G^GT[ACGT]ACC
FspI 644 TGCGCA TGC^GCA
HgaI 1196 GCGTC GACGC(5/10)
Hin1I 1195 GGCGTC G[GA]^CG[CT]C
HincII 1129 GTCAAC GT[CT]^[GA]AC
HindII 1129 GTCAAC GT[CT]^[GA]AC
MspA1I 922 CAGCTG C[AC]G^C[GT]G
MvnI 142 CGCG CG^CG
PaeR7I 1476 CTCGAG C^TCGAG
PflFI 1149 GACCGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PstI 42 CTGCAG CTGCA^G
PvuII 922 CAGCTG CAG^CTG
RcaI 602 TCATGA T^CATGA
SacI 241 GAGCTC GAGCT^C
SexAI 1247 ACCTGGT A^CC[AT]GGT
SfaNI 706 GATGC GCATC(5/9)
SspBI 935 TGTACA T^GTACA
StuI 765 AGGCCT AGG^CCT
Tth111I 1149 GACCGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 1663 TCTAGA T^CTAGA
XcmI 1397 CCAAGTAAGCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 1476 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AvaII 480 GGTCC G^G[AT]CC
1504 GGTCC
BanI 463 GGTGCC G^G[CT][GA]CC
1285 GGTGCC
BanII 241 GAGCTC G[GA]GC[CT]^C
1582 GAGCCC
BglII 10 AGATCT A^GATCT
128 AGATCT
BsaI 75 GAGACC GGTCTC(1/5)
184 GGTCTC
BseRI 1327 CTCCTC GAGGAG(10/8)
1342 GAGGAG
BsgI 620 GTGCAG GTGCAG(16/14)
1028 GTGCAG
BsrFI 17 GCCGGC [GA]^CCGG[CT]
1369 GCCGGC
BssSI 1457 CTCGTG CACGAG(-5/-1)
1680 CTCGTG
Cfr10I 17 GCCGGC [GA]^CCGG[CT]
1369 GCCGGC
FauI 143 GCGGG CCCGC(4/6)
731 GCGGG
HaeII 1304 AGCGCT [GA]GCGC^[CT]
1518 AGCGCC
NaeI 17 GCCGGC GCC^GGC
1369 GCCGGC
NgoMIV 17 GCCGGC G^CCGGC
1369 GCCGGC
PflMI 1038 CCAGGTAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1184 CCAATGGTTGG
PpuMI 479 GGGTCCC [GA]G^G[AT]CC[CT]
1503 GGGTCCC
SfcI 42 CTGCAG C^T[GA][CT]AG
1561 CTACAG
SmlI 109 CTCAAG C^T[CT][GA]AG
1476 CTCGAG
TfiI 147 GAATC G^A[AT]TC
1034 GAATC
Tsp509I 1335 AATT ^AATT
1617 AATT
Van91I 1038 CCAGGTAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1184 CCAATGGTTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 75 enzymes) #
#########################################
AciI AfaI AluI AlwI AspHI
BalI BbvI BcnI BfaI BmyI
BpmI BsaJI BsiHKAI BsiYI BslI
BsmAI BsmFI Bsp1286I BsrI BssKI
BstF5I BstNI BstXI BstYI Cac8I
CfoI Cfr13I DdeI DpnII DraII
EaeI EarI EcoO109I EcoRII EcoT14I
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MslI MspI MvaI MwoI NciI
NdeII NlaIII NlaIV PleI RsaI
Sau3AI Sau96I ScrFI StyI TaqI
TseI Tsp45I TspRI TthHB8I XhoII