##################################
# NO CUT ENZYMES ( 107 enzymes) #
##################################
AatII AccI AccIII AclI AflII
AgeI AhdI Alw44I AocI Aor51HI
ApaLI AscI AseI AsnI AspEI
AspI AviII BbeI BcgI BciVI
BfrI BsaBI BsaWI BseAI BsiEI
BsiWI BsmBI BspDI BspEI BspHI
BspLU11I BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstZ17I Bsu36I
ClaI CpoI DraI EagI Eam1105I
EarI EclXI Eco47III Eco52I Eco81I
EcoO65I EcoRI EcoRV FseI FspI
HaeII HincII HindII HindIII HpaI
KasI Ksp632I KspI MamI MluI
MroI NaeI NarI NdeI NgoMIV
NotI NruI NspI NspV PacI
PaeR7I PflFI PflMI PinAI PmeI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SacI SacII SalI SapI
SbfI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspI
SwaI Tth111I Van91I XbaI XhoI
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 63 enzymes) #
###################################
Acc65I 801 GGTACC G^GTACC
AccII 1560 CGCG CG^CG
AcyI 452 GACGCC G[GA]^CG[CT]C
AlwNI 522 CAGGTACTG CAG[ACGT][ACGT][ACGT]^CTG
Asp700I 695 GAAGCGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 801 GGTACC G^GTACC
AspHI 1629 GTGCTC G[AT]GC[AT]^C
AvaI 1606 CTCGGG C^[CT]CG[GA]G
BalI 1321 TGGCCA TGG^CCA
BamHI 1715 GGATCC G^GATCC
BbrPI 1420 CACGTG CAC^GTG
BbsI 1931 GAAGAC GAAGAC(2/6)
BclI 1462 TGATCA T^GATCA
BcnI 839 CCCGG CC^[GC]GG
BglII 657 AGATCT A^GATCT
BlpI 324 GCTGAGC GC^T[ACGT]AGC
BpmI 612 CTGGAG CTGGAG(16/14)
Bpu1102I 324 GCTGAGC GC^T[ACGT]AGC
BpuAI 1931 GAAGAC GAAGAC(2/6)
BsaAI 1420 CACGTG [CT]AC^GT[GA]
BsaHI 452 GACGCC G[GA]^CG[CT]C
BsiHKAI 1629 GTGCTC G[AT]GC[AT]^C
BsoBI 1606 CTCGGG C^[CT]CG[GA]G
Bsp1407I 652 TGTACA T^GTACA
BsrBI 1686 CCGCTC CCGCTC(-3/-3)
BsrDI 758 GCAATG GCAATG(2/0)
BsrFI 1588 GCCGGT [GA]^CCGG[CT]
BsrGI 652 TGTACA T^GTACA
BstUI 1560 CGCG CG^CG
CelII 324 GCTGAGC GC^T[ACGT]AGC
CfoI 73 GCGC GCG^C
Cfr10I 1588 GCCGGT [GA]^CCGG[CT]
DrdI 1170 GACTGGCAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 293 CCTTTTACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT22I 854 ATGCAT ATGCA^T
FbaI 1462 TGATCA T^GATCA
HhaI 73 GCGC GCG^C
Hin1I 452 GACGCC G[GA]^CG[CT]C
HinP1I 73 GCGC G^CGC
KpnI 801 GGTACC GGTAC^C
MfeI 111 CAATTG C^AATTG
MluNI 1321 TGGCCA TGG^CCA
MscI 1321 TGGCCA TGG^CCA
MseI 29 TTAA T^TAA
MunI 111 CAATTG C^AATTG
MvnI 1560 CGCG CG^CG
NciI 839 CCCGG CC^[GC]GG
NheI 1830 GCTAGC G^CTAGC
NsiI 854 ATGCAT ATGCA^T
PmaCI 1420 CACGTG CAC^GTG
PmlI 1420 CACGTG CAC^GTG
PpuMI 1786 AGGTCCC [GA]G^G[AT]CC[CT]
PstI 828 CTGCAG CTGCA^G
ScaI 940 AGTACT AGT^ACT
SexAI 646 ACCTGGT A^CC[AT]GGT
SfiI 1727 GGCCCCCCAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SspBI 652 TGTACA T^GTACA
StuI 1735 AGGCCT AGG^CCT
TaqI 1443 TCGA T^CGA
TfiI 430 GAATC G^A[AT]TC
Tru9I 29 TTAA T^TAA
TthHB8I 1443 TCGA T^CGA
XmnI 695 GAAGCGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 14 enzymes) #
###################################
AflIII 1419 ACACGT A^C[GA][CT]GT
1536 ACACGT
BanI 643 GGCACC G^G[CT][GA]CC
801 GGTACC
BbvCI 1705 CCTCAGC CCTCAGC(-5/-2)
461 GCTGAGG
BglI 1279 GCCTCTATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1728 GCCCCCCAGGC
BseRI 1025 CTCCTC GAGGAG(10/8)
1821 CTCCTC
BsmI 852 GAATGC GAATGC(1/-1)
1598 GAATGC
BstXI 626 CCAGGGTGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1645 CCACACAACTGG
DraIII 207 CACTGCGTG CAC[ACGT][ACGT][ACGT]^GTG
645 CACCTGGTG
DsaI 126 CCATGG C^C[GA][CT]GG
1498 CCATGG
EaeI 332 TGGCCG [CT]^GGCC[GA]
1321 TGGCCA
MaeII 1421 ACGT A^CGT
1538 ACGT
NcoI 126 CCATGG C^CATGG
1498 CCATGG
SfcI 384 CTACAG C^T[GA][CT]AG
828 CTGCAG
XcmI 139 CCACTTCTTCTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1498 CCATGGAGCAGCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 74 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApaI ApoI AvaII AvrII BanII
BbvI BfaI BlnI BmyI BsaI
BsaJI BsgI BsiYI BslI BsmAI
BsmFI Bsp1286I BspMI BsrI BssKI
BstF5I BstNI BstYI Cac8I Cfr13I
DdeI DpnII DraII EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeIII
HapII HgaI HinfI HpaII HphI
ItaI MaeI MaeIII MboI MboII
MflI MnlI MslI MspA1I MspI
MvaI MwoI NdeII NlaIII NlaIV
PleI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SmlI StyI TseI
Tsp45I Tsp509I TspRI XhoII