##################################
# NO CUT ENZYMES ( 112 enzymes) #
##################################
AatII AccI AccIII AclI AflII
AflIII AgeI AhdI Alw44I AocI
Aor51HI ApaLI AscI AseI AsnI
AspEI AspI AviII AvrII BalI
BamHI BbeI BbsI BbvCI BciVI
BclI BfrI BlnI BlpI Bpu1102I
BpuAI BsaBI BsaWI BseAI BseRI
BsiWI BspDI BspEI BspHI BspLU11I
BsrBI BsrDI BssHII Bst1107I BstAPI
BstBI BstZ17I Bsu36I CelII ClaI
CpoI DraI DraIII DrdI Eam1105I
Eco47III Eco81I EcoRV EcoT22I FbaI
FseI FspI HaeII HincII HindII
HpaI KasI MamI MfeI MluI
MluNI MroI MscI MseI MunI
NarI NdeI NheI NotI NruI
NsiI NspV PacI PflFI PinAI
PleI PmeI PshBI Psp1406I PvuI
RcaI RsrII SacI SalI SbfI
ScaI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI Sse8387I SspI
StuI SwaI Tru9I Tth111I XbaI
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 48 enzymes) #
###################################
Acc65I 1388 GGTACC G^GTACC
AcsI 427 GAATTC [GA]^AATT[CT]
AcyI 611 GGCGTC G[GA]^CG[CT]C
ApoI 427 GAATTC [GA]^AATT[CT]
Asp718I 1388 GGTACC G^GTACC
BanI 1388 GGTACC G^G[CT][GA]CC
BbrPI 139 CACGTG CAC^GTG
BcgI 696 GCATCACCATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglI 32 GCCGCGGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 287 AGATCT A^GATCT
BsaAI 139 CACGTG [CT]AC^GT[GA]
BsaHI 611 GGCGTC G[GA]^CG[CT]C
BsiEI 39 CGGCCG CG[GA][CT]^CG
BsmBI 967 GAGACG CGTCTC(1/5)
BsmI 1578 GCATTC GAATGC(1/-1)
Bsp1407I 1430 TGTACA T^GTACA
BsrFI 662 GCCGGC [GA]^CCGG[CT]
BsrGI 1430 TGTACA T^GTACA
BssSI 208 CTCGTG CACGAG(-5/-1)
BstXI 80 CCATGGCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr10I 662 GCCGGC [GA]^CCGG[CT]
EagI 39 CGGCCG C^GGCCG
EclXI 39 CGGCCG C^GGCCG
Eco52I 39 CGGCCG C^GGCCG
EcoNI 234 CCTGCGAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 427 GAATTC G^AATTC
HgaI 612 GCGTC GACGC(5/10)
Hin1I 611 GGCGTC G[GA]^CG[CT]C
HindIII 1128 AAGCTT A^AGCTT
KpnI 1388 GGTACC GGTAC^C
KspI 33 CCGCGG CCGC^GG
MaeII 140 ACGT A^CGT
NaeI 662 GCCGGC GCC^GGC
NcoI 80 CCATGG C^CATGG
NgoMIV 662 GCCGGC G^CCGGC
PaeR7I 410 CTCGAG C^TCGAG
PflMI 267 CCAGCCCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 139 CACGTG CAC^GTG
PmlI 139 CACGTG CAC^GTG
PshAI 1621 GACCTCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 180 CTGCAG CTGCA^G
SacII 33 CCGCGG CCGC^GG
SapI 556 GAAGAGC GCTCTTC(1/4)
SmlI 410 CTCGAG C^T[CT][GA]AG
SspBI 1430 TGTACA T^GTACA
Tsp509I 428 AATT ^AATT
Van91I 267 CCAGCCCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 410 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
ApaI 707 GGGCCC GGGCC^C
1010 GGGCCC
Asp700I 285 GAAGATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
423 GAAGGAATTC
AspHI 973 GAGCAC G[AT]GC[AT]^C
1217 GTGCTC
BpmI 124 CTGGAG CTGGAG(16/14)
360 CTGGAG
BsaI 413 GAGACC GGTCTC(1/5)
580 GGTCTC
BsiHKAI 973 GAGCAC G[AT]GC[AT]^C
1217 GTGCTC
BspMI 1470 ACCTGC ACCTGC(4/8)
1151 GCAGGT
BstEII 682 GGTCACC G^GT[ACGT]ACC
1164 GGTGACC
BstPI 682 GGTCACC G^GT[ACGT]ACC
1164 GGTGACC
EcoO65I 682 GGTCACC G^GT[ACGT]ACC
1164 GGTGACC
EcoT14I 80 CCATGG C^C[AT][AT]GG
1645 CCTTGG
HinfI 1424 GAATC G^A[ACGT]TC
1526 GAATC
MslI 81 CATGGCTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
135 CACGCACGTG
SexAI 440 ACCAGGT A^CC[AT]GGT
1391 ACCAGGT
SfaNI 563 GATGC GCATC(5/9)
696 GCATC
SfcI 52 CTACAG C^T[GA][CT]AG
180 CTGCAG
SphI 887 GCATGC GCATG^C
1707 GCATGC
StyI 80 CCATGG C^C[AT][AT]GG
1645 CCTTGG
TfiI 1424 GAATC G^A[AT]TC
1526 GAATC
XcmI 1335 CCAGGTCGGGTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1374 CCAGAAGCTAGATGG
XmnI 285 GAAGATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
423 GAAGGAATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 77 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AlwNI AvaI AvaII BanII BbvI
BcnI BfaI BmyI BsaJI BsgI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BsrI BssKI BstF5I BstNI
BstUI BstYI Cac8I CfoI Cfr13I
DdeI DpnII DraII DsaI EaeI
EarI EcoO109I EcoRII FauI Fnu4HI
FokI HaeIII HapII HhaI HinP1I
HpaII HphI ItaI Ksp632I MaeI
MaeIII MboI MboII MflI MnlI
MspA1I MspI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV NspI
PpuMI PvuII RsaI Sau3AI Sau96I
ScrFI TaqI TseI Tsp45I TspRI
TthHB8I XhoII