##################################
# NO CUT ENZYMES ( 94 enzymes) #
##################################
AccI AclI AcsI AflII AgeI
AhdI ApoI AscI AseI AsnI
AspEI AspI AviII AvrII BbrPI
BcgI BclI BfaI BfrI BlnI
BsaAI BsaBI BsiWI BsmBI BsmI
Bsp1407I BspDI BsrDI BsrGI BssHII
BssSI Bst1107I BstBI BstZ17I ClaI
CpoI DraI DraIII DrdI Eam1105I
EcoRI EcoRV EcoT22I FbaI FseI
FspI HincII HindII HpaI KspI
MaeI MamI MluI NaeI NdeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI RsrII SacII
SalI SapI SbfI ScaI SfuI
SgrAI SmlI SnaBI SpeI SphI
Sse8387I SspBI SspI SwaI TfiI
Tth111I Van91I XbaI XhoI
###################################
# ONE CUT ENZYMES ( 48 enzymes) #
###################################
AatII 944 GACGTC GACGT^C
Acc65I 488 GGTACC G^GTACC
AflIII 542 ACATGT A^C[GA][CT]GT
Alw44I 1715 GTGCAC G^TGCAC
ApaLI 1715 GTGCAC G^TGCAC
Asp700I 1888 GAAGACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 488 GGTACC G^GTACC
BalI 321 TGGCCA TGG^CCA
BamHI 1598 GGATCC G^GATCC
BbeI 1590 GGCGCC GGCGC^C
BbvCI 1191 CCTCAGC CCTCAGC(-5/-2)
BglI 803 GCCTGCGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 35 AGATCT A^GATCT
BsaI 1762 GGTCTC GGTCTC(1/5)
BsiEI 1358 CGGCCG CG[GA][CT]^CG
BspHI 459 TCATGA T^CATGA
BspLU11I 542 ACATGT A^CATGT
BsrBI 566 CCGCTC CCGCTC(-3/-3)
BsrFI 1101 GCCGGT [GA]^CCGG[CT]
BstAPI 1798 GCAACCACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Cfr10I 1101 GCCGGT [GA]^CCGG[CT]
EagI 1358 CGGCCG C^GGCCG
EarI 1022 CTCTTC CTCTTC(1/4)
EclXI 1358 CGGCCG C^GGCCG
Eco52I 1358 CGGCCG C^GGCCG
HindIII 2150 AAGCTT A^AGCTT
HinfI 1970 GACTC G^A[ACGT]TC
KasI 1590 GGCGCC G^GCGCC
KpnI 488 GGTACC GGTAC^C
Ksp632I 1022 CTCTTC CTCTTC(1/4)
MaeII 945 ACGT A^CGT
MfeI 2015 CAATTG C^AATTG
MluNI 321 TGGCCA TGG^CCA
MscI 321 TGGCCA TGG^CCA
MseI 2158 TTAA T^TAA
MslI 537 CATCGACATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MunI 2015 CAATTG C^AATTG
NarI 1590 GGCGCC GG^CGCC
NcoI 1288 CCATGG C^CATGG
NspI 542 ACATGT [GA]CATG^[CT]
PleI 1970 GACTC GAGTC(4/5)
PvuII 1069 CAGCTG CAG^CTG
RcaI 459 TCATGA T^CATGA
SfiI 802 GGCCTGCGGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 1590 GGCGCC GGC^GCC
Tru9I 2158 TTAA T^TAA
Tsp509I 2016 AATT ^AATT
XmnI 1888 GAAGACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AccII 925 CGCG CG^CG
1572 CGCG
AccIII 98 TCCGGA T^CCGGA
1250 TCCGGA
AocI 578 CCTCAGG CC^T[ACGT]AGG
628 CCTCAGG
Aor51HI 14 AGCGCT AGC^GCT
1487 AGCGCT
BciVI 2022 GGATAC GTATCC(6/5)
1898 GTATCC
BsaWI 98 TCCGGA [AT]^CCGG[AT]
1250 TCCGGA
BseAI 98 TCCGGA T^CCGGA
1250 TCCGGA
BsmAI 2031 GAGAC GTCTC(1/5)
1763 GTCTC
BspEI 98 TCCGGA T^CCGGA
1250 TCCGGA
BspMI 385 GCAGGT ACCTGC(4/8)
1730 GCAGGT
BstEII 561 GGTTACC G^GT[ACGT]ACC
605 GGTCACC
BstPI 561 GGTTACC G^GT[ACGT]ACC
605 GGTCACC
BstUI 925 CGCG CG^CG
1572 CGCG
BstXI 324 CCAGCCTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
678 CCAGGGAGGTGG
Bsu36I 578 CCTCAGG CC^T[ACGT]AGG
628 CCTCAGG
Eco47III 14 AGCGCT AGC^GCT
1487 AGCGCT
Eco81I 578 CCTCAGG CC^T[ACGT]AGG
628 CCTCAGG
EcoNI 578 CCTCAGGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1203 CCTCCCATAGG
EcoO65I 561 GGTTACC G^GT[ACGT]ACC
605 GGTCACC
EcoT14I 1288 CCATGG C^C[AT][AT]GG
1613 CCTTGG
MroI 98 TCCGGA T^CCGGA
1250 TCCGGA
MspA1I 1069 CAGCTG C[AC]G^C[GT]G
1839 CCGCTG
MvnI 925 CGCG CG^CG
1572 CGCG
SacI 1377 GAGCTC GAGCT^C
1417 GAGCTC
SexAI 609 ACCTGGT A^CC[AT]GGT
1535 ACCAGGT
StuI 801 AGGCCT AGG^CCT
1018 AGGCCT
StyI 1288 CCATGG C^C[AT][AT]GG
1613 CCTTGG
TaqI 539 TCGA T^CGA
1055 TCGA
TthHB8I 539 TCGA T^CGA
1055 TCGA
XcmI 1220 CCAAGCTGCCACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2038 CCAGGAGAGCCCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 86 enzymes) #
#########################################
AciI AcyI AfaI AluI AlwI
AlwNI ApaI AspHI AvaI AvaII
BanI BanII BbsI BbvI BcnI
BlpI BmyI BpmI Bpu1102I BpuAI
BsaHI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmFI BsoBI Bsp1286I
BsrI BssKI BstF5I BstNI BstYI
Cac8I CelII CfoI Cfr13I DdeI
DpnII DraII DsaI EaeI EcoO109I
EcoRII FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HpaII HphI ItaI MaeIII
MboI MboII MflI MnlI MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV PpuMI PstI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmaI
TseI Tsp45I TspRI XhoII XmaCI
XmaI