##################################
# NO CUT ENZYMES ( 120 enzymes) #
##################################
AatII Acc65I AccI AclI AcsI
AflII AflIII AgeI AhdI Alw44I
Aor51HI ApaI ApaLI ApoI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI AviII AvrII BbeI BbrPI
BbvCI BcgI BciVI BfaI BfrI
BglII BlnI BlpI Bpu1102I BsaAI
BsaBI BsiEI BsiWI BspDI BspHI
BspLU11I BsrDI BsrFI Bst1107I BstBI
BstEII BstPI BstZ17I CelII Cfr10I
ClaI CpoI DraI DraIII DrdI
EagI Eam1105I EclXI Eco47III Eco52I
EcoNI EcoO65I EcoRI EcoRV EcoT22I
FseI FspI HaeII HincII HindII
HindIII HpaI KasI KpnI KspI
MaeI MamI MfeI MluI MunI
NaeI NarI NdeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
RcaI RsrII SacI SacII SalI
SbfI SfiI SfoI SfuI SgrAI
SnaBI SpeI Sse8387I SspI StuI
SwaI Tth111I XbaI XhoI XmnI
###################################
# ONE CUT ENZYMES ( 40 enzymes) #
###################################
AccIII 275 TCCGGA T^CCGGA
AocI 206 CCTCAGG CC^T[ACGT]AGG
BamHI 908 GGATCC G^GATCC
BglI 1038 GCCCCCCCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaI 1418 GGTCTC GGTCTC(1/5)
BsaWI 275 TCCGGA [AT]^CCGG[AT]
BseAI 275 TCCGGA T^CCGGA
BsgI 958 CTGCAC GTGCAG(16/14)
BsmI 352 GAATGC GAATGC(1/-1)
Bsp1407I 820 TGTACA T^GTACA
BspEI 275 TCCGGA T^CCGGA
BspMI 705 GCAGGT ACCTGC(4/8)
BsrBI 1131 GAGCGG CCGCTC(-3/-3)
BsrGI 820 TGTACA T^GTACA
BssHII 375 GCGCGC G^CGCGC
BssSI 1060 CTCGTG CACGAG(-5/-1)
BstAPI 347 GCACGGAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 491 CCAAGGTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 206 CCTCAGG CC^T[ACGT]AGG
DraII 494 AGGTCCT [GA]G^G[ACGT]CC[CT]
DsaI 1031 CCATGG C^C[GA][CT]GG
Eco81I 206 CCTCAGG CC^T[ACGT]AGG
EcoO109I 494 AGGTCCT [GA]G^G[ACGT]CC[CT]
MaeII 194 ACGT A^CGT
MroI 275 TCCGGA T^CCGGA
NcoI 1031 CCATGG C^CATGG
NspI 701 GCATGC [GA]CATG^[CT]
PflMI 1107 CCAGTTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 494 AGGTCCT [GA]G^G[AT]CC[CT]
ScaI 998 AGTACT AGT^ACT
SexAI 179 ACCTGGT A^CC[AT]GGT
SmaI 713 CCCGGG CCC^GGG
SphI 701 GCATGC GCATG^C
SspBI 820 TGTACA T^GTACA
TaqI 1194 TCGA T^CGA
TfiI 654 GATTC G^A[AT]TC
TthHB8I 1194 TCGA T^CGA
Van91I 1107 CCAGTTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI 713 CCCGGG C^CCGGG
XmaI 713 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AcyI 49 GACGCC G[GA]^CG[CT]C
1026 GACGCC
AspHI 945 GTGCTC G[AT]GC[AT]^C
1090 GTGCTC
AvaI 634 CCCGAG C^[CT]CG[GA]G
713 CCCGGG
BalI 500 TGGCCA TGG^CCA
1151 TGGCCA
BanI 1012 GGCACC G^G[CT][GA]CC
1035 GGTGCC
BclI 1124 TGATCA T^GATCA
1549 TGATCA
BsaHI 49 GACGCC G[GA]^CG[CT]C
1026 GACGCC
BsiHKAI 945 GTGCTC G[AT]GC[AT]^C
1090 GTGCTC
BsmBI 47 GAGACG CGTCTC(1/5)
1024 GAGACG
BsoBI 634 CCCGAG C^[CT]CG[GA]G
713 CCCGGG
BstYI 67 GGATCT [GA]^GATC[CT]
908 GGATCC
EaeI 500 TGGCCA [CT]^GGCC[GA]
1151 TGGCCA
EarI 235 CTCTTC CTCTTC(1/4)
271 CTCTTC
FbaI 1124 TGATCA T^GATCA
1549 TGATCA
Hin1I 49 GACGCC G[GA]^CG[CT]C
1026 GACGCC
Ksp632I 235 CTCTTC CTCTTC(1/4)
271 CTCTTC
MflI 67 GGATCT [GA]^GATC[CT]
908 GGATCC
MluNI 500 TGGCCA TGG^CCA
1151 TGGCCA
MscI 500 TGGCCA TGG^CCA
1151 TGGCCA
MseI 260 TTAA T^TAA
1721 TTAA
MspA1I 316 CAGCTG C[AC]G^C[GT]G
806 CAGCTG
PstI 288 CTGCAG CTGCA^G
1573 CTGCAG
PvuII 316 CAGCTG CAG^CTG
806 CAGCTG
SapI 234 GCTCTTC GCTCTTC(1/4)
270 GCTCTTC
Tru9I 260 TTAA T^TAA
1721 TTAA
XcmI 503 CCAAGAAAGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
526 CCAATTATCGGCTGG
XhoII 67 GGATCT [GA]^GATC[CT]
908 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 71 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AlwNI AvaII BanII BbsI BbvI
BcnI BmyI BpmI BpuAI BsaJI
BseRI BsiYI BslI BsmAI BsmFI
Bsp1286I BsrI BssKI BstF5I BstNI
BstUI Cac8I CfoI Cfr13I DdeI
DpnII EcoRII EcoT14I FauI Fnu4HI
FokI HaeIII HapII HgaI HhaI
HinP1I HinfI HpaII HphI ItaI
MaeIII MboI MboII MnlI MslI
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV PleI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI StyI TseI Tsp45I Tsp509I
TspRI