##################################
# NO CUT ENZYMES ( 105 enzymes) #
##################################
AatII AccIII AclI AflII AgeI
Alw44I AocI Aor51HI ApaLI AscI
AseI AsnI AspI AviII AvrII
BamHI BbrPI BbsI BcgI BciVI
BfrI BglII BlnI BlpI Bpu1102I
BpuAI BsaAI BsaBI BseAI BsiWI
BspDI BspEI BspHI BspLU11I BsrDI
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstZ17I Bsu36I CelII ClaI
CpoI DraI DrdI Eco47III Eco81I
EcoO65I EcoRV EcoT22I FseI FspI
HindIII HpaI KspI MamI MfeI
MluI MroI MunI NdeI NheI
NotI NsiI NspI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PvuI RcaI RsrII SacI SacII
SapI SbfI ScaI SexAI SfiI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SphI Sse8387I SspI StuI
SwaI Tth111I XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 41 enzymes) #
###################################
Acc65I 1919 GGTACC G^GTACC
AflIII 1711 ACACGT A^C[GA][CT]GT
AhdI 836 GACGTGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI 1290 CAGTGACTG CAG[ACGT][ACGT][ACGT]^CTG
Asp700I 1444 GAATGTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 1919 GGTACC G^GTACC
AspEI 836 GACGTGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI 473 CCCGAG C^[CT]CG[GA]G
BbeI 220 GGCGCC GGCGC^C
BclI 1637 TGATCA T^GATCA
BsaWI 732 TCCGGT [AT]^CCGG[AT]
BsmBI 476 GAGACG CGTCTC(1/5)
BsmI 1313 GCATTC GAATGC(1/-1)
BsoBI 473 CCCGAG C^[CT]CG[GA]G
Bsp1407I 1362 TGTACA T^GTACA
BsrBI 1665 CCGCTC CCGCTC(-3/-3)
BsrGI 1362 TGTACA T^GTACA
BssHII 529 GCGCGC G^CGCGC
BstYI 1055 AGATCC [GA]^GATC[CT]
DsaI 1179 CCATGG C^C[GA][CT]GG
Eam1105I 836 GACGTGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 1256 CTCTTC CTCTTC(1/4)
EcoRI 1087 GAATTC G^AATTC
FbaI 1637 TGATCA T^GATCA
HincII 467 GTCGAC GT[CT]^[GA]AC
HindII 467 GTCGAC GT[CT]^[GA]AC
KasI 220 GGCGCC G^GCGCC
KpnI 1919 GGTACC GGTAC^C
Ksp632I 1256 CTCTTC CTCTTC(1/4)
MflI 1055 AGATCC [GA]^GATC[CT]
NarI 220 GGCGCC GG^CGCC
NcoI 1179 CCATGG C^CATGG
NruI 607 TCGCGA TCG^CGA
SalI 467 GTCGAC G^TCGAC
SfoI 220 GGCGCC GGC^GCC
SspBI 1362 TGTACA T^GTACA
TaqI 468 TCGA T^CGA
TfiI 1355 GAATC G^A[AT]TC
TthHB8I 468 TCGA T^CGA
XhoII 1055 AGATCC [GA]^GATC[CT]
XmnI 1444 GAATGTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AccI 467 GTCGAC GT^[AC][GT]AC
1132 GTCTAC
AcyI 220 GGCGCC G[GA]^CG[CT]C
503 GGCGTC
AspHI 6 GTGCTC G[AT]GC[AT]^C
941 GAGCAC
BalI 1176 TGGCCA TGG^CCA
1182 TGGCCA
BsaHI 220 GGCGCC G[GA]^CG[CT]C
503 GGCGTC
BsiHKAI 6 GTGCTC G[AT]GC[AT]^C
941 GAGCAC
BstXI 873 CCAAGCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1569 CCAGGATCATGG
DraIII 1286 CACCCAGTG CAC[ACGT][ACGT][ACGT]^GTG
1498 CACTGAGTG
EcoT14I 1026 CCTTGG C^C[AT][AT]GG
1179 CCATGG
HgaI 206 GACGC GACGC(5/10)
504 GCGTC
Hin1I 220 GGCGCC G[GA]^CG[CT]C
503 GGCGTC
MluNI 1176 TGGCCA TGG^CCA
1182 TGGCCA
MscI 1176 TGGCCA TGG^CCA
1182 TGGCCA
MslI 944 CACCTGAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1818 CATGGCAGTG
NaeI 334 GCCGGC GCC^GGC
562 GCCGGC
NgoMIV 334 GCCGGC G^CCGGC
562 GCCGGC
PstI 491 CTGCAG CTGCA^G
1654 CTGCAG
PvuII 488 CAGCTG CAG^CTG
1954 CAGCTG
SfaNI 429 GCATC GCATC(5/9)
1683 GCATC
SfcI 491 CTGCAG C^T[GA][CT]AG
1654 CTGCAG
StyI 1026 CCTTGG C^C[AT][AT]GG
1179 CCATGG
Tsp45I 1068 GTGAC ^GT[GC]AC
1292 GTGAC
XbaI 1406 TCTAGA T^CTAGA
1760 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 89 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI ApaI ApoI AvaII BanI
BanII BbvCI BbvI BcnI BfaI
BglI BmyI BpmI BsaI BsaJI
BseRI BsgI BsiEI BsiYI BslI
BsmAI BsmFI Bsp1286I BspMI BsrFI
BsrI BssKI BstF5I BstNI BstUI
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraII EaeI EagI EclXI
Eco52I EcoNI EcoO109I EcoRII FauI
Fnu4HI FokI HaeII HaeIII HapII
HhaI HinP1I HinfI HpaII HphI
ItaI MaeI MaeII MaeIII MboI
MboII MnlI MseI MspA1I MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV PflMI PleI RsaI
Sau3AI Sau96I ScrFI Tru9I TseI
Tsp509I TspRI Van91I XcmI