##################################
# NO CUT ENZYMES ( 135 enzymes) #
##################################
AatII Acc65I AccII AclI AcsI
AcyI AflII AgeI AhdI AlwNI
Aor51HI ApoI AscI AseI AsnI
Asp700I Asp718I AspEI AspI AviII
AvrII BalI BamHI BbeI BbsI
BbvCI BcgI BciVI BclI BfrI
BlnI BlpI Bpu1102I BpuAI BsaBI
BsaHI BsiEI BsiWI BsmFI Bsp1407I
BspDI BspHI BsrDI BsrFI BsrGI
BssHII BssSI BstBI BstEII BstPI
BstUI CelII CfoI Cfr10I ClaI
CpoI DraI EaeI EagI Eam1105I
EarI EclXI Eco47III Eco52I EcoO65I
EcoRI EcoRV EcoT22I FbaI FseI
FspI HaeII HhaI Hin1I HinP1I
HincII HindII HpaI KasI KpnI
Ksp632I KspI MamI MfeI MluI
MluNI MscI MspA1I MunI MvnI
NaeI NarI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PinAI PmeI PshAI PshBI
Psp1406I PstI PvuI PvuII RcaI
RsrII SacII SalI SapI SbfI
SexAI SfcI SfoI SfuI SgrAI
SmaI SmlI SnaBI SpeI Sse8387I
SspBI StuI SwaI Tth111I XbaI
XcmI XhoI XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 46 enzymes) #
###################################
AccI 1534 GTATAC GT^[AC][GT]AC
AccIII 1043 TCCGGA T^CCGGA
Alw44I 1562 GTGCAC G^TGCAC
ApaI 356 GGGCCC GGGCC^C
ApaLI 1562 GTGCAC G^TGCAC
AvaI 387 CTCGGG C^[CT]CG[GA]G
BbrPI 848 CACGTG CAC^GTG
BcnI 315 CCGGG CC^[GC]GG
BfaI 1396 CTAG C^TAG
BglI 358 GCCCAGTCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 932 AGATCT A^GATCT
BsaAI 848 CACGTG [CT]AC^GT[GA]
BsaWI 1043 TCCGGA [AT]^CCGG[AT]
BseAI 1043 TCCGGA T^CCGGA
BsmBI 9 GAGACG CGTCTC(1/5)
BsmI 859 GCATTC GAATGC(1/-1)
BsoBI 387 CTCGGG C^[CT]CG[GA]G
BspEI 1043 TCCGGA T^CCGGA
BspMI 1551 ACCTGC ACCTGC(4/8)
BsrBI 384 CCGCTC CCGCTC(-3/-3)
Bst1107I 1534 GTATAC GTA^TAC
BstAPI 1555 GCACGTTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 705 CCAAGGCGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 1534 GTATAC GTA^TAC
DraIII 1556 CACGTTGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 139 GACACTTTTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 552 CCTTCATCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HgaI 907 GCGTC GACGC(5/10)
HindIII 1048 AAGCTT A^AGCTT
MaeI 1396 CTAG C^TAG
MroI 1043 TCCGGA T^CCGGA
NciI 315 CCGGG CC^[GC]GG
NcoI 1138 CCATGG C^CATGG
PflMI 258 CCAGGATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 2 GAGTC GAGTC(4/5)
PmaCI 848 CACGTG CAC^GTG
PmlI 848 CACGTG CAC^GTG
PpuMI 1338 GGGTCCT [GA]G^G[AT]CC[CT]
SacI 893 GAGCTC GAGCT^C
ScaI 1442 AGTACT AGT^ACT
SfiI 357 GGCCCAGTCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SphI 1054 GCATGC GCATG^C
SspI 495 AATATT AAT^ATT
TaqI 33 TCGA T^CGA
TthHB8I 33 TCGA T^CGA
Van91I 258 CCAGGATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AlwI 632 GATCC GGATC(4/5)
789 GATCC
AocI 79 CCTCAGG CC^T[ACGT]AGG
617 CCTGAGG
AspHI 893 GAGCTC G[AT]GC[AT]^C
1562 GTGCAC
BanI 27 GGCACC G^G[CT][GA]CC
1130 GGTGCC
BseRI 620 GAGGAG GAGGAG(10/8)
814 GAGGAG
BsiHKAI 893 GAGCTC G[AT]GC[AT]^C
1562 GTGCAC
BspLU11I 1530 ACATGT A^CATGT
1566 ACATGT
BstYI 631 AGATCC [GA]^GATC[CT]
932 AGATCT
Bsu36I 79 CCTCAGG CC^T[ACGT]AGG
617 CCTGAGG
DsaI 462 CCGTGG C^C[GA][CT]GG
1138 CCATGG
Eco81I 79 CCTCAGG CC^T[ACGT]AGG
617 CCTGAGG
FauI 383 CCCGC CCCGC(4/6)
1457 GCGGG
HapII 315 CCGG C^CGG
1044 CCGG
HpaII 315 CCGG C^CGG
1044 CCGG
MflI 631 AGATCC [GA]^GATC[CT]
932 AGATCT
MslI 848 CACGTGGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1518 CACCAACATG
MspI 315 CCGG C^CGG
1044 CCGG
NdeI 236 CATATG CA^TATG
1539 CATATG
Tsp509I 512 AATT ^AATT
759 AATT
XhoII 631 AGATCC [GA]^GATC[CT]
932 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 57 enzymes) #
#########################################
AciI AfaI AflIII AluI AvaII
BanII BbvI BmyI BpmI BsaI
BsaJI BsgI BsiYI BslI BsmAI
Bsp1286I BsrI BssKI BstF5I BstNI
Cac8I Cfr13I DdeI DpnII DraII
EcoO109I EcoRII EcoT14I Fnu4HI FokI
HaeIII HinfI HphI ItaI MaeII
MaeIII MboI MboII MnlI MseI
MvaI MwoI NdeII NlaIII NlaIV
NspI RsaI Sau3AI Sau96I ScrFI
SfaNI StyI TfiI Tru9I TseI
Tsp45I TspRI