##################################
# NO CUT ENZYMES ( 128 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcyI AflII AgeI AhdI Alw44I
AlwNI AocI Aor51HI ApaI ApaLI
AscI Asp700I Asp718I AspEI AvaI
AvrII BamHI BanI BbeI BbsI
BcgI BciVI BclI BfrI BglI
BlnI BlpI Bpu1102I BpuAI BsaHI
BsaWI BseAI BsiEI BsiWI BsmI
BsoBI BspDI BspEI BspMI BsrFI
BssHII BssSI Bst1107I BstAPI BstEII
BstPI BstXI BstZ17I Bsu36I Cac8I
CelII Cfr10I ClaI CpoI DrdI
DsaI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoNI EcoO65I EcoRV
EcoT22I FauI FbaI FseI HaeII
HgaI Hin1I HpaI KasI KpnI
KspI MfeI MluI MroI MspA1I
MunI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
PacI PaeR7I PflMI PinAI PmeI
PpuMI PshAI Psp1406I PstI PvuI
PvuII RsrII SacII SalI SbfI
SexAI SfiI SfoI SgrAI SmaI
SnaBI SpeI SphI Sse8387I StuI
SwaI Van91I XbaI XcmI XhoI
XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 50 enzymes) #
###################################
AccII 1053 CGCG CG^CG
AseI 682 ATTAAT AT^TAAT
AsnI 682 ATTAAT AT^TAAT
AviII 1196 TGCGCA TGC^GCA
BanII 1467 GAGCTC G[GA]GC[CT]^C
BbrPI 1705 CACGTG CAC^GTG
BcnI 737 CCCGG CC^[GC]GG
BsaAI 1705 CACGTG [CT]AC^GT[GA]
BsaBI 724 GATAATGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 1126 GAGACC GGTCTC(1/5)
BsgI 1289 CTGCAC GTGCAG(16/14)
BsmBI 1156 CGTCTC CGTCTC(1/5)
BsmFI 1723 GGGAC GGGAC(10/14)
Bsp1407I 535 TGTACA T^GTACA
BspHI 336 TCATGA T^CATGA
BsrBI 1518 GAGCGG CCGCTC(-3/-3)
BsrGI 535 TGTACA T^GTACA
BstBI 1489 TTCGAA TT^CGAA
BstUI 1053 CGCG CG^CG
DraII 1433 AGGCCCC [GA]G^G[ACGT]CC[CT]
DraIII 4 CACAATGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoO109I 1433 AGGCCCC [GA]G^G[ACGT]CC[CT]
EcoRI 1492 GAATTC G^AATTC
FspI 1196 TGCGCA TGC^GCA
HapII 738 CCGG C^CGG
HincII 635 GTCAAC GT[CT]^[GA]AC
HindII 635 GTCAAC GT[CT]^[GA]AC
HindIII 780 AAGCTT A^AGCTT
HpaII 738 CCGG C^CGG
HphI 1283 TCACC GGTGA(8/7)
MaeII 1706 ACGT A^CGT
MamI 724 GATAATGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MspI 738 CCGG C^CGG
MvnI 1053 CGCG CG^CG
NciI 737 CCCGG CC^[GC]GG
NlaIV 1434 GGCCCC GG[ACGT]^[ACGT]CC
NspV 1489 TTCGAA TT^CGAA
PmaCI 1705 CACGTG CAC^GTG
PmlI 1705 CACGTG CAC^GTG
PshBI 682 ATTAAT AT^TAAT
RcaI 336 TCATGA T^CATGA
SacI 1467 GAGCTC GAGCT^C
SapI 328 GCTCTTC GCTCTTC(1/4)
ScaI 137 AGTACT AGT^ACT
SfuI 1489 TTCGAA TT^CGAA
SspBI 535 TGTACA T^GTACA
SspI 966 AATATT AAT^ATT
TaqI 1490 TCGA T^CGA
Tsp45I 980 GTCAC ^GT[GC]AC
TthHB8I 1490 TCGA T^CGA
###################################
# TWO CUT ENZYMES ( 16 enzymes) #
###################################
AspI 1317 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
1523 GACCTTGTC
BglII 1018 AGATCT A^GATCT
1278 AGATCT
BpmI 1294 CTCCAG CTGGAG(16/14)
92 CTGGAG
BseRI 1392 GAGGAG GAGGAG(10/8)
1464 GAGGAG
BsiYI 649 CCAGCCTGGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
738 CCGGAGCTAGG
BslI 649 CCAGCCTGGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
738 CCGGAGCTAGG
BsrI 250 ACTGG ACTGG(1/-1)
135 CCAGT
CfoI 1052 GCGC GCG^C
1197 GCGC
EcoT14I 857 CCTTGG C^C[AT][AT]GG
1452 CCAAGG
HhaI 1052 GCGC GCG^C
1197 GCGC
HinP1I 1052 GCGC G^CGC
1197 GCGC
MslI 283 CACGCTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1777 CATTTATGTG
PflFI 1317 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
1523 GACCTTGTC
SfcI 27 CTATAG C^T[GA][CT]AG
1203 CTGTAG
StyI 857 CCTTGG C^C[AT][AT]GG
1452 CCAAGG
Tth111I 1317 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
1523 GACCTTGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 64 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
AlwI ApoI AspHI AvaII BalI
BbvCI BbvI BfaI BmyI BsaJI
BsiHKAI BsmAI Bsp1286I BspLU11I BsrDI
BssKI BstF5I BstNI BstYI Cfr13I
DdeI DpnII DraI EaeI EarI
EcoRII Fnu4HI FokI HaeIII HinfI
ItaI Ksp632I MaeI MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MvaI MwoI NdeII NlaIII
NspI PleI RsaI Sau3AI Sau96I
ScrFI SfaNI SmlI TfiI Tru9I
TseI Tsp509I TspRI XhoII