##################################
# NO CUT ENZYMES ( 114 enzymes) #
##################################
AatII AccIII AcyI AflII AgeI
AhdI Alw44I Aor51HI ApaI ApaLI
AscI AseI AsnI Asp700I AspEI
AspI AvaI AviII AvrII BamHI
BbeI BbrPI BcgI BclI BfrI
BlnI BlpI Bpu1102I BsaAI BsaHI
BsaWI BseAI BsiEI BsiWI BsmBI
BsmI BsoBI BspDI BspEI BspHI
BsrBI BssHII BssSI Bst1107I BstBI
BstEII BstPI BstZ17I CelII ClaI
CpoI DrdI EagI Eam1105I EclXI
Eco47III Eco52I EcoO65I EcoT22I FbaI
FseI FspI HaeII Hin1I HpaI
KasI KspI MfeI MluI MroI
MunI NaeI NarI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PshBI PvuI
RcaI RsrII SacII SalI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SwaI Tth111I Van91I XcmI
XhoI XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 40 enzymes) #
###################################
Acc65I 320 GGTACC G^GTACC
AccI 1983 GTCTAC GT^[AC][GT]AC
AclI 441 AACGTT AA^CGTT
AflIII 54 ACATGT A^C[GA][CT]GT
Asp718I 320 GGTACC G^GTACC
BalI 1921 TGGCCA TGG^CCA
BbvCI 1943 CCTCAGC CCTCAGC(-5/-2)
BciVI 252 GTATCC GTATCC(6/5)
BsgI 1246 GTGCAG GTGCAG(16/14)
Bsp1407I 264 TGTACA T^GTACA
BspLU11I 54 ACATGT A^CATGT
BsrFI 623 GCCGGT [GA]^CCGG[CT]
BsrGI 264 TGTACA T^GTACA
BstAPI 1843 GCAGGAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 2033 CCATGGTGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr10I 623 GCCGGT [GA]^CCGG[CT]
DraI 350 TTTAAA TTT^AAA
DraIII 480 CACTCAGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 1921 TGGCCA [CT]^GGCC[GA]
EcoNI 1968 CCTGAGCTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 399 GAATTC G^AATTC
EcoRV 710 GATATC GAT^ATC
FauI 1260 CCCGC CCCGC(4/6)
HgaI 1897 GCGTC GACGC(5/10)
HincII 223 GTTGAC GT[CT]^[GA]AC
HindII 223 GTTGAC GT[CT]^[GA]AC
HindIII 1457 AAGCTT A^AGCTT
KpnI 320 GGTACC GGTAC^C
MluNI 1921 TGGCCA TGG^CCA
MscI 1921 TGGCCA TGG^CCA
NspI 54 ACATGT [GA]CATG^[CT]
PpuMI 735 AGGACCT [GA]G^G[AT]CC[CT]
PshAI 1991 GACATAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 441 AACGTT AA^CGTT
PstI 1502 CTGCAG CTGCA^G
SacI 1186 GAGCTC GAGCT^C
SapI 1058 GAAGAGC GCTCTTC(1/4)
SmlI 896 CTCAAG C^T[CT][GA]AG
SspBI 264 TGTACA T^GTACA
XbaI 403 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AlwNI 371 CAGGAGCTG CAG[ACGT][ACGT][ACGT]^CTG
1844 CAGGAGCTG
AocI 739 CCTCAGG CC^T[ACGT]AGG
1138 CCTGAGG
BanI 320 GGTACC G^G[CT][GA]CC
1294 GGTGCC
BglII 295 AGATCT A^GATCT
1014 AGATCT
BsaBI 290 GATCGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1652 GATTACAATC
BsaI 803 GAGACC GGTCTC(1/5)
1008 GGTCTC
BsmFI 433 GGGAC GGGAC(10/14)
2003 GGGAC
BspMI 825 GCAGGT ACCTGC(4/8)
1426 GCAGGT
Bsu36I 739 CCTCAGG CC^T[ACGT]AGG
1138 CCTGAGG
DraII 735 AGGACCT [GA]G^G[ACGT]CC[CT]
1142 AGGCCCT
Eco81I 739 CCTCAGG CC^T[ACGT]AGG
1138 CCTGAGG
EcoO109I 735 AGGACCT [GA]G^G[ACGT]CC[CT]
1142 AGGCCCT
MaeII 363 ACGT A^CGT
442 ACGT
MamI 290 GATCGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1652 GATTACAATC
MspA1I 1352 CAGCTG C[AC]G^C[GT]G
1902 CAGCTG
NcoI 580 CCATGG C^CATGG
2033 CCATGG
PvuII 1352 CAGCTG CAG^CTG
1902 CAGCTG
SfcI 703 CTACAG C^T[GA][CT]AG
1502 CTGCAG
SspI 147 AATATT AAT^ATT
356 AATATT
StuI 1527 AGGCCT AGG^CCT
1803 AGGCCT
TaqI 292 TCGA T^CGA
1999 TCGA
TfiI 203 GAATC G^A[AT]TC
758 GAATC
Tsp45I 1334 GTGAC ^GT[GC]AC
1479 GTGAC
TthHB8I 292 TCGA T^CGA
1999 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 80 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI ApoI AspHI AvaII BanII
BbsI BbvI BcnI BfaI BglI
BmyI BpmI BpuAI BsaJI BseRI
BsiHKAI BsiYI BslI BsmAI Bsp1286I
BsrDI BsrI BssKI BstF5I BstNI
BstUI BstYI Cac8I CfoI Cfr13I
DdeI DpnII DsaI EarI EcoRII
EcoT14I Fnu4HI FokI HaeIII HapII
HhaI HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeIII MboI
MboII MflI MnlI MseI MslI
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV PleI RsaI
Sau3AI Sau96I ScrFI SfaNI StyI
Tru9I TseI Tsp509I TspRI XhoII