##################################
# NO CUT ENZYMES ( 98 enzymes) #
##################################
Acc65I AccI AccIII AclI Aor51HI
AscI AseI AsnI Asp700I Asp718I
AviII AvrII BalI BamHI BbeI
BcgI BciVI BclI BglII BlnI
BlpI Bpu1102I BsaBI BseAI BseRI
BsiWI BsmBI BspDI BspEI BspLU11I
BssHII BssSI Bst1107I BstBI BstZ17I
CelII ClaI CpoI DraI DraIII
DrdI Eco47III EcoRI EcoRV EcoT22I
FbaI FseI FspI HaeII HindIII
KasI KpnI MamI MfeI MluI
MluNI MroI MscI MunI NaeI
NarI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PmeI
PpuMI PshAI PshBI Psp1406I PstI
PvuI RsrII SacI SalI SbfI
ScaI SexAI SfcI SfiI SfoI
SfuI SgrAI SnaBI SpeI Sse8387I
SspI StuI SwaI TfiI XbaI
XcmI XhoI XmnI
###################################
# ONE CUT ENZYMES ( 60 enzymes) #
###################################
AatII 467 GACGTC GACGT^C
AcsI 1089 AAATTT [GA]^AATT[CT]
AcyI 467 GACGTC G[GA]^CG[CT]C
AflII 229 CTTAAG C^TTAAG
AflIII 1588 ACGTGT A^C[GA][CT]GT
AgeI 1166 ACCGGT A^CCGGT
AhdI 410 GACCGAGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 904 GTGCAC G^TGCAC
AocI 1377 CCTGAGG CC^T[ACGT]AGG
ApaLI 904 GTGCAC G^TGCAC
ApoI 1089 AAATTT [GA]^AATT[CT]
AspEI 410 GACCGAGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 904 GTGCAC G[AT]GC[AT]^C
AspI 1259 GACGAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaII 643 GGTCC G^G[AT]CC
BbrPI 1268 CACGTG CAC^GTG
BbvCI 1149 GCTGAGG CCTCAGC(-5/-2)
BfrI 229 CTTAAG C^TTAAG
BpmI 695 CTGGAG CTGGAG(16/14)
BsaHI 467 GACGTC G[GA]^CG[CT]C
BsgI 503 GTGCAG GTGCAG(16/14)
BsiHKAI 904 GTGCAC G[AT]GC[AT]^C
BsmI 126 GCATTC GAATGC(1/-1)
Bsp1407I 3 TGTACA T^GTACA
BspHI 1537 TCATGA T^CATGA
BsrDI 330 CATTGC GCAATG(2/0)
BsrGI 3 TGTACA T^GTACA
BstAPI 906 GCACCGGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 1294 GGTGACC G^GT[ACGT]ACC
BstPI 1294 GGTGACC G^GT[ACGT]ACC
Bsu36I 1377 CCTGAGG CC^T[ACGT]AGG
Eam1105I 410 GACCGAGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 1377 CCTGAGG CC^T[ACGT]AGG
EcoNI 157 CCTCATCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 1294 GGTGACC G^GT[ACGT]ACC
Hin1I 467 GACGTC G[GA]^CG[CT]C
HpaI 875 GTTAAC GTT^AAC
MslI 1343 CACTCCTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NcoI 87 CCATGG C^CATGG
NdeI 1222 CATATG CA^TATG
NspI 796 GCATGC [GA]CATG^[CT]
PflFI 1259 GACGAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 990 CCATCTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 1166 ACCGGT A^CCGGT
PmaCI 1268 CACGTG CAC^GTG
PmlI 1268 CACGTG CAC^GTG
RcaI 1537 TCATGA T^CATGA
SapI 96 GAAGAGC GCTCTTC(1/4)
SfaNI 42 GCATC GCATC(5/9)
SmaI 1492 CCCGGG CCC^GGG
SmlI 229 CTTAAG C^T[CT][GA]AG
SphI 796 GCATGC GCATG^C
SspBI 3 TGTACA T^GTACA
TaqI 1366 TCGA T^CGA
Tsp509I 1090 AATT ^AATT
Tth111I 1259 GACGAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 1366 TCGA T^CGA
Van91I 990 CCATCTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI 1492 CCCGGG C^CCGGG
XmaI 1492 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
ApaI 1333 GGGCCC GGGCC^C
1495 GGGCCC
BanI 670 GGCACC G^G[CT][GA]CC
1465 GGTGCC
BanII 1333 GGGCCC G[GA]GC[CT]^C
1495 GGGCCC
BbsI 772 GAAGAC GAAGAC(2/6)
470 GTCTTC
BfaI 728 CTAG C^TAG
1102 CTAG
BglI 341 GCCACCGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1555 GCCCACAGGGC
BpuAI 772 GAAGAC GAAGAC(2/6)
470 GTCTTC
BsaAI 1268 CACGTG [CT]AC^GT[GA]
1587 TACGTG
BsaI 1181 GAGACC GGTCTC(1/5)
985 GGTCTC
BsaWI 1166 ACCGGT [AT]^CCGG[AT]
1546 TCCGGT
BsmAI 1181 GAGAC GTCTC(1/5)
986 GTCTC
BspMI 1155 GCAGGT ACCTGC(4/8)
1361 GCAGGT
BsrBI 1506 CCGCTC CCGCTC(-3/-3)
854 GAGCGG
BsrFI 908 ACCGGC [GA]^CCGG[CT]
1166 ACCGGT
BstXI 1029 CCAAGGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1134 CCAGCACTTTGG
BstYI 171 GGATCT [GA]^GATC[CT]
837 AGATCC
Cfr10I 908 ACCGGC [GA]^CCGG[CT]
1166 ACCGGT
EcoT14I 87 CCATGG C^C[AT][AT]GG
1029 CCAAGG
KspI 345 CCGCGG CCGC^GG
710 CCGCGG
MaeI 728 CTAG C^TAG
1102 CTAG
MflI 171 GGATCT [GA]^GATC[CT]
837 AGATCC
SacII 345 CCGCGG CCGC^GG
710 CCGCGG
StyI 87 CCATGG C^C[AT][AT]GG
1029 CCAAGG
XhoII 171 GGATCT [GA]^GATC[CT]
837 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 76 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AlwNI AvaI BbvI BcnI BmyI
BsaJI BsiEI BsiYI BslI BsmFI
BsoBI Bsp1286I BsrI BssKI BstF5I
BstNI BstUI Cac8I CfoI Cfr13I
DdeI DpnII DraII DsaI EaeI
EagI EarI EclXI Eco52I EcoO109I
EcoRII FauI Fnu4HI FokI HaeIII
HapII HgaI HhaI HinP1I HincII
HindII HinfI HpaII HphI ItaI
Ksp632I MaeII MaeIII MboI MboII
MnlI MseI MspA1I MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV PleI PvuII RsaI Sau3AI
Sau96I ScrFI Tru9I TseI Tsp45I
TspRI