##################################
# NO CUT ENZYMES ( 76 enzymes) #
##################################
Acc65I AccI AccIII AclI Alw44I
ApaLI AscI AseI AsnI Asp718I
AviII BbeI BbvCI BcgI BciVI
BclI BsaBI BsaI BseAI BsiWI
BsmI BspDI BspEI BspLU11I BssHII
Bst1107I BstAPI BstBI BstZ17I ClaI
CpoI DraIII EagI EclXI Eco52I
EcoRI EcoRV EcoT22I FbaI FseI
FspI KasI KpnI MamI MluI
MroI NaeI NarI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PmeI PshAI PshBI
Psp1406I PvuI PvuII RsrII SalI
SapI SexAI SfiI SfoI SfuI
SnaBI SpeI SwaI XbaI XcmI
XhoI
###################################
# ONE CUT ENZYMES ( 55 enzymes) #
###################################
AatII 2068 GACGTC GACGT^C
AflII 1322 CTTAAG C^TTAAG
AflIII 2022 ACACGT A^C[GA][CT]GT
AgeI 2219 ACCGGT A^CCGGT
AhdI 704 GACGGGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Asp700I 1798 GAAGCCGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 704 GACGGGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 2526 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 2274 CCTAGG C^CTAGG
BamHI 2683 GGATCC G^GATCC
BfrI 1322 CTTAAG C^TTAAG
BglII 1098 AGATCT A^GATCT
BlnI 2274 CCTAGG C^CTAGG
BlpI 2360 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 2360 GCTCAGC GC^T[ACGT]AGC
BsaWI 2219 ACCGGT [AT]^CCGG[AT]
BsgI 1232 CTGCAC GTGCAG(16/14)
BsiEI 1634 CGACCG CG[GA][CT]^CG
BsmBI 2070 CGTCTC CGTCTC(1/5)
Bsp1407I 544 TGTACA T^GTACA
BspHI 2292 TCATGA T^CATGA
BsrDI 673 GCAATG GCAATG(2/0)
BsrGI 544 TGTACA T^GTACA
CelII 2360 GCTCAGC GC^T[ACGT]AGC
DraI 1013 TTTAAA TTT^AAA
DrdI 445 GACAAGAGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 704 GACGGGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 2195 CTCTTC CTCTTC(1/4)
HindIII 1135 AAGCTT A^AGCTT
HpaI 1151 GTTAAC GTT^AAC
Ksp632I 2195 CTCTTC CTCTTC(1/4)
KspI 1932 CCGCGG CCGC^GG
MfeI 480 CAATTG C^AATTG
MunI 480 CAATTG C^AATTG
NspI 997 GCATGC [GA]CATG^[CT]
PflFI 2526 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 2219 ACCGGT A^CCGGT
RcaI 2292 TCATGA T^CATGA
SacI 1705 GAGCTC GAGCT^C
SacII 1932 CCGCGG CCGC^GG
SbfI 2322 CCTGCAGG CCTGCA^GG
ScaI 404 AGTACT AGT^ACT
SgrAI 2218 CACCGGTG C[GA]^CCGG[CT]G
SmaI 1772 CCCGGG CCC^GGG
SphI 997 GCATGC GCATG^C
Sse8387I 2322 CCTGCAGG CCTGCA^GG
SspBI 544 TGTACA T^GTACA
SspI 1430 AATATT AAT^ATT
StuI 1054 AGGCCT AGG^CCT
TaqI 1923 TCGA T^CGA
Tth111I 2526 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 1923 TCGA T^CGA
XmaCI 1772 CCCGGG C^CCGGG
XmaI 1772 CCCGGG C^CCGGG
XmnI 1798 GAAGCCGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AlwNI 1002 CAGCTTCTG CAG[ACGT][ACGT][ACGT]^CTG
2317 CAGACCCTG
BbrPI 2023 CACGTG CAC^GTG
2731 CACGTG
BglI 1327 GCCCTTCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1632 GCCGACCGGGC
BsaAI 2023 CACGTG [CT]AC^GT[GA]
2731 CACGTG
BssSI 16 CTCGTG CACGAG(-5/-1)
100 CTCGTG
BstEII 154 GGTAACC G^GT[ACGT]ACC
1909 GGTCACC
BstPI 154 GGTAACC G^GT[ACGT]ACC
1909 GGTCACC
BstXI 236 CCATCACTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2654 CCATGTCCCTGG
EcoO65I 154 GGTAACC G^GT[ACGT]ACC
1909 GGTCACC
HincII 1151 GTTAAC GT[CT]^[GA]AC
1226 GTTGAC
HindII 1151 GTTAAC GT[CT]^[GA]AC
1226 GTTGAC
NcoI 1181 CCATGG C^CATGG
1650 CCATGG
PflMI 1061 CCATGCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1562 CCAGTTGATGG
PleI 1310 GACTC GAGTC(4/5)
2443 GAGTC
PmaCI 2023 CACGTG CAC^GTG
2731 CACGTG
PmlI 2023 CACGTG CAC^GTG
2731 CACGTG
PpuMI 1926 AGGTCCC [GA]G^G[AT]CC[CT]
2250 GGGACCC
TfiI 2190 GAATC G^A[AT]TC
2514 GAATC
Van91I 1061 CCATGCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1562 CCAGTTGATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 108 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AluI AlwI AocI Aor51HI ApaI
ApoI AspHI AvaI AvaII BalI
BanI BanII BbsI BbvI BcnI
BfaI BmyI BpmI BpuAI BsaHI
BsaJI BseRI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BspMI
BsrBI BsrFI BsrI BssKI BstF5I
BstNI BstUI BstYI Bsu36I Cac8I
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DsaI EaeI Eco47III Eco81I
EcoNI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV PstI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI StyI Tru9I TseI Tsp45I
Tsp509I TspRI XhoII