##################################
# NO CUT ENZYMES ( 99 enzymes) #
##################################
AclI AflII AflIII AgeI AhdI
Aor51HI AscI AseI AsnI Asp700I
AspEI AspI AviII AvrII BbeI
BbvCI BcgI BclI BfrI BlnI
BlpI Bpu1102I BsiEI BsiWI BsmI
BspDI BspLU11I BsrBI BssHII BssSI
Bst1107I BstBI BstXI BstZ17I CelII
ClaI CpoI DraI EagI Eam1105I
EclXI Eco47III Eco52I EcoRI EcoRV
EcoT22I FbaI FseI FspI HindIII
HpaI KasI KspI MfeI MluI
MseI MunI NarI NcoI NdeI
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PinAI PmeI
PshAI PshBI Psp1406I PvuI RsrII
SacI SacII SalI SapI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI Sse8387I
SspI SwaI Tru9I Tth111I XbaI
XhoI XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 40 enzymes) #
###################################
AatII 370 GACGTC GACGT^C
Acc65I 1567 GGTACC G^GTACC
AccIII 1961 TCCGGA T^CCGGA
AcsI 331 AAATTC [GA]^AATT[CT]
Alw44I 381 GTGCAC G^TGCAC
ApaLI 381 GTGCAC G^TGCAC
ApoI 331 AAATTC [GA]^AATT[CT]
Asp718I 1567 GGTACC G^GTACC
AspHI 381 GTGCAC G[AT]GC[AT]^C
BamHI 36 GGATCC G^GATCC
BbrPI 1263 CACGTG CAC^GTG
BbsI 178 GTCTTC GAAGAC(2/6)
BciVI 2111 GTATCC GTATCC(6/5)
BpuAI 178 GTCTTC GAAGAC(2/6)
BsaWI 1961 TCCGGA [AT]^CCGG[AT]
BseAI 1961 TCCGGA T^CCGGA
BsgI 786 CTGCAC GTGCAG(16/14)
BsiHKAI 381 GTGCAC G[AT]GC[AT]^C
BsmBI 615 CGTCTC CGTCTC(1/5)
BspEI 1961 TCCGGA T^CCGGA
BspMI 1878 GCAGGT ACCTGC(4/8)
BsrFI 89 GCCGGC [GA]^CCGG[CT]
BstAPI 1480 GCAGGCCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 483 GGTCACC G^GT[ACGT]ACC
BstPI 483 GGTCACC G^GT[ACGT]ACC
Cfr10I 89 GCCGGC [GA]^CCGG[CT]
DraIII 11 CACCCAGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoO65I 483 GGTCACC G^GT[ACGT]ACC
KpnI 1567 GGTACC GGTAC^C
MroI 1961 TCCGGA T^CCGGA
NaeI 89 GCCGGC GCC^GGC
NgoMIV 89 GCCGGC G^CCGGC
PmaCI 1263 CACGTG CAC^GTG
PmlI 1263 CACGTG CAC^GTG
SmlI 1093 CTCAAG C^T[CT][GA]AG
SphI 1017 GCATGC GCATG^C
TaqI 419 TCGA T^CGA
Tsp509I 332 AATT ^AATT
TthHB8I 419 TCGA T^CGA
XcmI 23 CCACCGCCCTGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 33 enzymes) #
###################################
AccII 214 CGCG CG^CG
461 CGCG
AcyI 370 GACGTC G[GA]^CG[CT]C
683 GACGCC
AocI 735 CCTGAGG CC^T[ACGT]AGG
2374 CCTGAGG
AvaI 769 CCCGAG C^[CT]CG[GA]G
898 CCCGAG
BanI 1567 GGTACC G^G[CT][GA]CC
1648 GGCACC
BglI 583 GCCAACCCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
2408 GCCCCTGGGGC
BglII 662 AGATCT A^GATCT
1046 AGATCT
BsaAI 625 TACGTG [CT]AC^GT[GA]
1263 CACGTG
BsaBI 61 GATGATGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
495 GATCTCCATC
BsaHI 370 GACGTC G[GA]^CG[CT]C
683 GACGCC
BsaI 1855 GAGACC GGTCTC(1/5)
970 GGTCTC
BsoBI 769 CCCGAG C^[CT]CG[GA]G
898 CCCGAG
Bsp1407I 452 TGTACA T^GTACA
1250 TGTACA
BsrDI 579 CATTGC GCAATG(2/0)
2106 GCAATG
BsrGI 452 TGTACA T^GTACA
1250 TGTACA
BstUI 214 CGCG CG^CG
461 CGCG
Bsu36I 735 CCTGAGG CC^T[ACGT]AGG
2374 CCTGAGG
DrdI 271 GACCTCCTCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
352 GACATCTTCGTC
DsaI 966 CCACGG C^C[GA][CT]GG
2326 CCACGG
Eco81I 735 CCTGAGG CC^T[ACGT]AGG
2374 CCTGAGG
EcoT14I 1644 CCTTGG C^C[AT][AT]GG
2068 CCTTGG
HgaI 683 GACGC GACGC(5/10)
2238 GACGC
Hin1I 370 GACGTC G[GA]^CG[CT]C
683 GACGCC
HincII 697 GTCAAC GT[CT]^[GA]AC
1279 GTCAAC
HindII 697 GTCAAC GT[CT]^[GA]AC
1279 GTCAAC
MamI 61 GATGATGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
495 GATCTCCATC
MspA1I 431 CAGCTG C[AC]G^C[GT]G
940 CAGCTG
MvnI 214 CGCG CG^CG
461 CGCG
PpuMI 509 GGGTCCT [GA]G^G[AT]CC[CT]
904 AGGACCC
PvuII 431 CAGCTG CAG^CTG
940 CAGCTG
SspBI 452 TGTACA T^GTACA
1250 TGTACA
StuI 1334 AGGCCT AGG^CCT
1447 AGGCCT
StyI 1644 CCTTGG C^C[AT][AT]GG
2068 CCTTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 86 enzymes) #
#########################################
AccI AciI AfaI AluI AlwI
AlwNI ApaI AvaII BalI BanII
BbvI BcnI BfaI BmyI BpmI
BsaJI BseRI BsiYI BslI BsmAI
BsmFI Bsp1286I BspHI BsrI BssKI
BstF5I BstNI BstYI Cac8I CfoI
Cfr13I DdeI DpnII DraII EaeI
EarI EcoNI EcoO109I EcoRII FauI
Fnu4HI FokI HaeII HaeIII HapII
HhaI HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MslI MspI MvaI MwoI
NciI NdeII NlaIII NlaIV NspI
PflMI PleI PstI RcaI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
TfiI TseI Tsp45I TspRI Van91I
XhoII