##################################
# NO CUT ENZYMES ( 84 enzymes) #
##################################
Acc65I AccIII AgeI Alw44I ApaLI
AscI AseI AsnI Asp718I AvrII
BalI BbeI BbvCI BcgI BglI
BglII BlnI BsaBI BseAI BsiEI
BsiWI BsmI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrGI BstBI ClaI
CpoI DraI EagI EclXI Eco52I
EcoRV EcoT22I FseI HincII HindII
HpaI KasI KpnI KspI MamI
MfeI MluI MluNI MroI MscI
MunI NaeI NarI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PinAI PmeI PshAI PshBI
PvuI RcaI RsrII SacII SalI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SpeI SphI Sse8387I
SspBI SspI SwaI XhoI
###################################
# ONE CUT ENZYMES ( 50 enzymes) #
###################################
AatII 1427 GACGTC GACGT^C
AccI 1267 GTATAC GT^[AC][GT]AC
AclI 423 AACGTT AA^CGTT
AflII 689 CTTAAG C^TTAAG
AflIII 2164 ACACGT A^C[GA][CT]GT
AhdI 17 GACTCCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 266 AGCGCT AGC^GCT
Asp700I 562 GAAGAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 17 GACTCCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 652 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 1173 TGCGCA TGC^GCA
BbrPI 2165 CACGTG CAC^GTG
BciVI 196 GGATAC GTATCC(6/5)
BclI 495 TGATCA T^GATCA
BfrI 689 CTTAAG C^TTAAG
BsaWI 603 ACCGGA [AT]^CCGG[AT]
BsrBI 326 GAGCGG CCGCTC(-3/-3)
BsrFI 402 ACCGGC [GA]^CCGG[CT]
BssHII 93 GCGCGC G^CGCGC
BssSI 1701 CACGAG CACGAG(-5/-1)
Bst1107I 1267 GTATAC GTA^TAC
BstAPI 1514 GCAACTGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 1990 GGTGACC G^GT[ACGT]ACC
BstPI 1990 GGTGACC G^GT[ACGT]ACC
BstZ17I 1267 GTATAC GTA^TAC
Cfr10I 402 ACCGGC [GA]^CCGG[CT]
DrdI 512 GACATGCAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 17 GACTCCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 266 AGCGCT AGC^GCT
EcoNI 2358 CCTCCTGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 1990 GGTGACC G^GT[ACGT]ACC
EcoRI 1379 GAATTC G^AATTC
FbaI 495 TGATCA T^GATCA
FspI 1173 TGCGCA TGC^GCA
HindIII 2279 AAGCTT A^AGCTT
MspA1I 1791 CAGCTG C[AC]G^C[GT]G
NdeI 1002 CATATG CA^TATG
PflFI 652 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 2165 CACGTG CAC^GTG
PmlI 2165 CACGTG CAC^GTG
PpuMI 519 AGGTCCT [GA]G^G[AT]CC[CT]
Psp1406I 423 AACGTT AA^CGTT
PvuII 1791 CAGCTG CAG^CTG
SmaI 1614 CCCGGG CCC^GGG
SnaBI 795 TACGTA TAC^GTA
StuI 2028 AGGCCT AGG^CCT
Tth111I 652 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmaCI 1614 CCCGGG C^CCGGG
XmaI 1614 CCCGGG C^CCGGG
XmnI 562 GAAGAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 33 enzymes) #
###################################
AcyI 204 GGCGTC G[GA]^CG[CT]C
1427 GACGTC
AfaI 794 GTAC GT^AC
798 GTAC
AocI 1589 CCTGAGG CC^T[ACGT]AGG
1915 CCTGAGG
ApaI 535 GGGCCC GGGCC^C
1960 GGGCCC
AvaI 462 CTCGGG C^[CT]CG[GA]G
1614 CCCGGG
BamHI 162 GGATCC G^GATCC
2257 GGATCC
BbsI 2312 GAAGAC GAAGAC(2/6)
1838 GTCTTC
BlpI 122 GCTCAGC GC^T[ACGT]AGC
262 GCTAAGC
Bpu1102I 122 GCTCAGC GC^T[ACGT]AGC
262 GCTAAGC
BpuAI 2312 GAAGAC GAAGAC(2/6)
1838 GTCTTC
BsaAI 795 TACGTA [CT]AC^GT[GA]
2165 CACGTG
BsaHI 204 GGCGTC G[GA]^CG[CT]C
1427 GACGTC
BsaI 1131 GAGACC GGTCTC(1/5)
1799 GAGACC
BsmBI 206 CGTCTC CGTCTC(1/5)
68 GAGACG
BsoBI 462 CTCGGG C^[CT]CG[GA]G
1614 CCCGGG
BstXI 697 CCACAAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1758 CCAAGTCCTTGG
Bsu36I 1589 CCTGAGG CC^T[ACGT]AGG
1915 CCTGAGG
CelII 122 GCTCAGC GC^T[ACGT]AGC
262 GCTAAGC
DraIII 2039 CACTGGGTG CAC[ACGT][ACGT][ACGT]^GTG
2165 CACGTGGTG
EaeI 951 CGGCCA [CT]^GGCC[GA]
1456 TGGCCG
EarI 29 CTCTTC CTCTTC(1/4)
1396 CTCTTC
Eco81I 1589 CCTGAGG CC^T[ACGT]AGG
1915 CCTGAGG
HaeII 266 AGCGCT [GA]GCGC^[CT]
1887 AGCGCC
Hin1I 204 GGCGTC G[GA]^CG[CT]C
1427 GACGTC
Ksp632I 29 CTCTTC CTCTTC(1/4)
1396 CTCTTC
MslI 1708 CATGGCTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1771 CATCCAGGTG
NcoI 1140 CCATGG C^CATGG
1707 CCATGG
PstI 1943 CTGCAG CTGCA^G
1996 CTGCAG
RsaI 794 GTAC GT^AC
798 GTAC
SacI 1046 GAGCTC GAGCT^C
1094 GAGCTC
SapI 28 GCTCTTC GCTCTTC(1/4)
1395 GCTCTTC
TfiI 754 GAATC G^A[AT]TC
825 GAATC
XbaI 13 TCTAGA T^CTAGA
1660 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 91 enzymes) #
#########################################
AccII AciI AcsI AluI AlwI
AlwNI ApoI AspHI AvaII BanI
BanII BbvI BcnI BfaI BmyI
BpmI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI Bsp1286I
BspMI BsrDI BsrI BssKI BstF5I
BstNI BstUI BstYI Cac8I CfoI
Cfr13I DdeI DpnII DraII DsaI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeIII HapII HgaI HhaI
HinP1I HinfI HpaII HphI ItaI
MaeI MaeII MaeIII MboI MboII
MflI MnlI MseI MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV NspI PflMI PleI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
StyI TaqI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I XcmI
XhoII