##################################
# NO CUT ENZYMES ( 106 enzymes) #
##################################
AatII AccIII AclI AcyI AflII
AgeI AhdI AocI AscI AseI
AsnI Asp700I AspEI AvaI AvrII
BbeI BbvCI BcgI BclI BfrI
BlnI BsaHI BseAI BsiEI BsiWI
BsoBI Bsp1407I BspDI BspEI BspHI
BsrFI BsrGI BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstXI BstZ17I
Bsu36I Cfr10I ClaI CpoI DraIII
DrdI EagI Eam1105I EclXI Eco52I
Eco81I EcoNI EcoO65I EcoRV FbaI
FseI Hin1I KasI KspI MfeI
MluI MroI MunI NaeI NarI
NcoI NdeI NgoMIV NheI NotI
NruI NspV PacI PaeR7I PinAI
PleI PmeI PshAI PshBI Psp1406I
PvuI RcaI RsrII SacI SacII
SalI SapI SbfI SexAI SfiI
SfoI SfuI SgrAI SmaI SmlI
SnaBI SphI Sse8387I SspBI SspI
SwaI XbaI XhoI XmaCI XmaI
XmnI
###################################
# ONE CUT ENZYMES ( 52 enzymes) #
###################################
Acc65I 276 GGTACC G^GTACC
AccI 1350 GTAGAC GT^[AC][GT]AC
AcsI 1081 GAATTC [GA]^AATT[CT]
Alw44I 1672 GTGCAC G^TGCAC
AlwNI 1211 CAGGGTCTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 66 AGCGCT AGC^GCT
ApaI 291 GGGCCC GGGCC^C
ApaLI 1672 GTGCAC G^TGCAC
ApoI 1081 GAATTC [GA]^AATT[CT]
Asp718I 276 GGTACC G^GTACC
AspI 1261 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 476 TGCGCA TGC^GCA
BamHI 83 GGATCC G^GATCC
BanI 276 GGTACC G^G[CT][GA]CC
BbrPI 215 CACGTG CAC^GTG
BbsI 1249 GTCTTC GAAGAC(2/6)
BciVI 774 GGATAC GTATCC(6/5)
BglI 426 GCCTTCGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 1635 GCTCAGC GC^T[ACGT]AGC
BpmI 1417 CTCCAG CTGGAG(16/14)
Bpu1102I 1635 GCTCAGC GC^T[ACGT]AGC
BpuAI 1249 GTCTTC GAAGAC(2/6)
BsaBI 458 GATCTGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseRI 1539 GAGGAG GAGGAG(10/8)
BsmI 1889 GAATGC GAATGC(1/-1)
BspLU11I 97 ACATGT A^CATGT
BsrDI 1608 GCAATG GCAATG(2/0)
BssHII 34 GCGCGC G^CGCGC
CelII 1635 GCTCAGC GC^T[ACGT]AGC
DraI 1026 TTTAAA TTT^AAA
EarI 263 GAAGAG CTCTTC(1/4)
Eco47III 66 AGCGCT AGC^GCT
EcoRI 1081 GAATTC G^AATTC
EcoT14I 1254 CCAAGG C^C[AT][AT]GG
EcoT22I 1168 ATGCAT ATGCA^T
FauI 1710 GCGGG CCCGC(4/6)
FspI 476 TGCGCA TGC^GCA
HaeII 66 AGCGCT [GA]GCGC^[CT]
HpaI 1880 GTTAAC GTT^AAC
KpnI 276 GGTACC GGTAC^C
Ksp632I 263 GAAGAG CTCTTC(1/4)
MamI 458 GATCTGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NsiI 1168 ATGCAT ATGCA^T
PflFI 1261 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 215 CACGTG CAC^GTG
PmlI 215 CACGTG CAC^GTG
PvuII 1383 CAGCTG CAG^CTG
ScaI 1099 AGTACT AGT^ACT
SpeI 1354 ACTAGT A^CTAGT
StuI 593 AGGCCT AGG^CCT
StyI 1254 CCAAGG C^C[AT][AT]GG
Tth111I 1261 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AccII 35 CGCG CG^CG
1751 CGCG
AflIII 97 ACATGT A^C[GA][CT]GT
902 ACACGT
AvaII 326 GGACC G^G[AT]CC
506 GGACC
BsaAI 215 CACGTG [CT]AC^GT[GA]
1189 CACGTA
BsaWI 454 ACCGGA [AT]^CCGG[AT]
909 ACCGGA
BsgI 632 GTGCAG GTGCAG(16/14)
1297 GTGCAG
BsmBI 791 CGTCTC CGTCTC(1/5)
1434 GAGACG
BsmFI 538 GGGAC GGGAC(10/14)
376 GTCCC
BspMI 508 ACCTGC ACCTGC(4/8)
1372 ACCTGC
BsrBI 6 CCGCTC CCGCTC(-3/-3)
24 CCGCTC
BstUI 35 CGCG CG^CG
1751 CGCG
DdeI 1035 CTCAG C^T[ACGT]AG
1636 CTCAG
DsaI 841 CCGTGG C^C[GA][CT]GG
1123 CCGTGG
HindIII 344 AAGCTT A^AGCTT
568 AAGCTT
MboII 263 GAAGA GAAGA(8/7)
1250 TCTTC
MslI 468 CACACCAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1687 CATGTGGGTG
MvnI 35 CGCG CG^CG
1751 CGCG
PpuMI 325 AGGACCT [GA]G^G[AT]CC[CT]
505 AGGACCT
PstI 48 CTGCAG CTGCA^G
62 CTGCAG
XcmI 401 CCAGGGCTCCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
934 CCATCCGCATCCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 80 enzymes) #
#########################################
AciI AfaI AluI AlwI AspHI
BalI BanII BbvI BcnI BfaI
BglII BmyI BsaI BsaJI BsiHKAI
BsiYI BslI BsmAI Bsp1286I BsrI
BssKI BstF5I BstNI BstYI Cac8I
CfoI Cfr13I DpnII DraII EaeI
EcoO109I EcoRII Fnu4HI FokI HaeIII
HapII HgaI HhaI HinP1I HincII
HindII HinfI HpaII HphI ItaI
MaeI MaeII MaeIII MboI MflI
MluNI MnlI MscI MseI MspA1I
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV NspI PflMI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I XhoII