##################################
# NO CUT ENZYMES ( 117 enzymes) #
##################################
AatII AccII AccIII AclI AcyI
AflII AflIII AgeI AhdI Aor51HI
ApaI AscI AseI AsnI Asp700I
AspEI AspI AviII AvrII BbeI
BbrPI BciVI BcnI BfrI BglII
BlnI BlpI Bpu1102I BsaBI BsaHI
BsaWI BseAI BsiEI BsiWI Bsp1407I
BspDI BspEI BspLU11I BsrFI BsrGI
BssHII BssSI Bst1107I BstEII BstPI
BstUI BstXI BstZ17I CelII Cfr10I
ClaI CpoI DraI DraIII EagI
Eam1105I EclXI Eco47III Eco52I EcoNI
EcoO65I EcoRV EcoT22I FseI FspI
HaeII Hin1I HpaI KasI KspI
MamI MfeI MluI MroI MunI
MvnI NaeI NarI NciI NdeI
NgoMIV NheI NotI NruI NsiI
NspI PacI PflFI PflMI PinAI
PmaCI PmeI PmlI PshBI Psp1406I
PvuI RsrII SacII SalI SbfI
SfiI SfoI SgrAI SmaI SpeI
SphI Sse8387I SspBI SspI StuI
SwaI Tth111I Van91I XbaI XmaCI
XmaI XmnI
###################################
# ONE CUT ENZYMES ( 36 enzymes) #
###################################
Acc65I 616 GGTACC G^GTACC
AccI 343 GTAGAC GT^[AC][GT]AC
Alw44I 97 GTGCAC G^TGCAC
AlwNI 2192 CAGTTCCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaLI 97 GTGCAC G^TGCAC
Asp718I 616 GGTACC G^GTACC
BamHI 2097 GGATCC G^GATCC
BanI 616 GGTACC G^G[CT][GA]CC
BbvCI 2008 GCTGAGG CCTCAGC(-5/-2)
BcgI 2223 CGATGAGGATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglI 130 GCCTTTCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaAI 60 TACGTA [CT]AC^GT[GA]
BsaI 1683 GGTCTC GGTCTC(1/5)
BspMI 1560 GCAGGT ACCTGC(4/8)
BsrBI 1891 GAGCGG CCGCTC(-3/-3)
BstAPI 139 GCAGAAATTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 270 TTCGAA TT^CGAA
DraII 2039 AGGACCT [GA]G^G[ACGT]CC[CT]
EcoO109I 2039 AGGACCT [GA]G^G[ACGT]CC[CT]
FauI 672 GCGGG CCCGC(4/6)
HapII 3 CCGG C^CGG
HincII 1225 GTTGAC GT[CT]^[GA]AC
HindII 1225 GTTGAC GT[CT]^[GA]AC
HindIII 1396 AAGCTT A^AGCTT
HpaII 3 CCGG C^CGG
KpnI 616 GGTACC GGTAC^C
MspI 3 CCGG C^CGG
NspV 270 TTCGAA TT^CGAA
PpuMI 2039 AGGACCT [GA]G^G[AT]CC[CT]
PshAI 1832 GACTTGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuII 1176 CAGCTG CAG^CTG
SacI 1324 GAGCTC GAGCT^C
ScaI 1640 AGTACT AGT^ACT
SexAI 2042 ACCTGGT A^CC[AT]GGT
SfuI 270 TTCGAA TT^CGAA
SnaBI 60 TACGTA TAC^GTA
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AspHI 97 GTGCAC G[AT]GC[AT]^C
1324 GAGCTC
AvaII 950 GGACC G^G[AT]CC
2040 GGACC
BalI 1565 TGGCCA TGG^CCA
1946 TGGCCA
BsgI 443 GTGCAG GTGCAG(16/14)
1648 GTGCAG
BsiHKAI 97 GTGCAC G[AT]GC[AT]^C
1324 GAGCTC
BsmBI 2234 CGTCTC CGTCTC(1/5)
1990 GAGACG
BspHI 1781 TCATGA T^CATGA
1898 TCATGA
BsrDI 396 CATTGC GCAATG(2/0)
1076 CATTGC
CfoI 6 GCGC GCG^C
1449 GCGC
Cfr13I 950 GGACC G^G[ACGT]CC
2040 GGACC
DrdI 1482 GACATCTCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1518 GACACAGAAGTC
EaeI 1565 TGGCCA [CT]^GGCC[GA]
1946 TGGCCA
EcoRI 991 GAATTC G^AATTC
1066 GAATTC
HgaI 1992 GACGC GACGC(5/10)
2233 GCGTC
HhaI 6 GCGC GCG^C
1449 GCGC
HinP1I 6 GCGC G^CGC
1449 GCGC
MluNI 1565 TGGCCA TGG^CCA
1946 TGGCCA
MscI 1565 TGGCCA TGG^CCA
1946 TGGCCA
MspA1I 864 CAGCGG C[AC]G^C[GT]G
1176 CAGCTG
PaeR7I 2215 CTCGAG C^TCGAG
2325 CTCGAG
PstI 2189 CTGCAG CTGCA^G
2318 CTGCAG
RcaI 1781 TCATGA T^CATGA
1898 TCATGA
Sau96I 950 GGACC G^G[ACGT]CC
2040 GGACC
TfiI 243 GATTC G^A[AT]TC
1403 GAATC
Tsp45I 1816 GTGAC ^GT[GC]AC
1858 GTGAC
XhoI 2215 CTCGAG C^TCGAG
2325 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 79 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AocI ApoI AvaI BanII BbsI
BbvI BclI BfaI BmyI BpmI
BpuAI BsaJI BseRI BsiYI BslI
BsmAI BsmFI BsmI BsoBI Bsp1286I
BsrI BssKI BstF5I BstNI BstYI
Bsu36I Cac8I DdeI DpnII DsaI
EarI Eco81I EcoRII EcoT14I FbaI
Fnu4HI FokI HaeIII HinfI HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MslI MvaI MwoI NcoI NdeII
NlaIII NlaIV PleI RsaI SapI
Sau3AI ScrFI SfaNI SfcI SmlI
StyI TaqI Tru9I TseI Tsp509I
TspRI TthHB8I XcmI XhoII