##################################
# NO CUT ENZYMES ( 105 enzymes) #
##################################
Acc65I AccI AccIII AclI AcsI
AflII AflIII AgeI AocI Aor51HI
ApoI AscI AseI AsnI Asp718I
AspI AviII AvrII BamHI BbeI
BbrPI BciVI BclI BfrI BglII
BlnI BlpI Bpu1102I BsaAI BsaI
BsaWI BseAI BsmBI BsmI Bsp1407I
BspEI BspLU11I BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstYI BstZ17I
Bsu36I CelII CpoI DraI DrdI
Eco47III Eco81I EcoRI EcoT22I FbaI
FspI HincII HindII HpaI KasI
KpnI KspI MfeI MflI MluI
MroI MunI NarI NdeI NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PshBI Psp1406I PstI PvuI
RsrII SacI SacII SalI SbfI
ScaI SfiI SfoI SfuI SgrAI
SnaBI SpeI Sse8387I SspBI SspI
SwaI Tth111I XbaI XhoI XhoII
###################################
# ONE CUT ENZYMES ( 44 enzymes) #
###################################
AfaI 791 GTAC GT^AC
AhdI 1314 GACTCCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 1907 GTGCAC G^TGCAC
AlwI 652 GGATC GGATC(4/5)
ApaLI 1907 GTGCAC G^TGCAC
Asp700I 1722 GAAAAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 1314 GACTCCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BbsI 214 GTCTTC GAAGAC(2/6)
BbvCI 890 CCTCAGC CCTCAGC(-5/-2)
BcgI 1924 CGAGGAAAGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI 1138 CTAG C^TAG
BpuAI 214 GTCTTC GAAGAC(2/6)
BsaBI 1073 GATGCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiWI 790 CGTACG C^GTACG
BsrDI 1528 CATTGC GCAATG(2/0)
BstEII 272 GGTCACC G^GT[ACGT]ACC
BstPI 272 GGTCACC G^GT[ACGT]ACC
DraIII 897 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 1314 GACTCCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 680 GAAGAG CTCTTC(1/4)
EcoO65I 272 GGTCACC G^GT[ACGT]ACC
EcoRV 543 GATATC GAT^ATC
FseI 695 GGCCGGCC GGCCGG^CC
HaeII 1095 AGCGCC [GA]GCGC^[CT]
HindIII 1725 AAGCTT A^AGCTT
Ksp632I 680 GAAGAG CTCTTC(1/4)
MaeI 1138 CTAG C^TAG
MamI 1073 GATGCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
PflMI 426 CCACCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 1341 AGGACCC [GA]G^G[AT]CC[CT]
PshAI 454 GACGCCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RsaI 791 GTAC GT^AC
SapI 680 GAAGAGC GCTCTTC(1/4)
SexAI 898 ACCTGGT A^CC[AT]GGT
SfcI 1717 CTATAG C^T[GA][CT]AG
SmaI 307 CCCGGG CCC^GGG
SmlI 1829 CTCAAG C^T[CT][GA]AG
SphI 487 GCATGC GCATG^C
TfiI 716 GAATC G^A[AT]TC
Tsp509I 1817 AATT ^AATT
Van91I 426 CCACCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI 307 CCCGGG C^CCGGG
XmaI 307 CCCGGG C^CCGGG
XmnI 1722 GAAAAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AatII 88 GACGTC GACGT^C
993 GACGTC
AlwNI 465 CAGCAGCTG CAG[ACGT][ACGT][ACGT]^CTG
1061 CAGCACCTG
AvaII 311 GGACC G^G[AT]CC
1342 GGACC
BalI 535 TGGCCA TGG^CCA
1936 TGGCCA
BsiEI 847 CGGCCG CG[GA][CT]^CG
974 CGGCCG
BsmAI 1268 GAGAC GTCTC(1/5)
1322 GTCTC
BspDI 540 ATCGAT AT^CGAT
1954 ATCGAT
BstXI 556 CCAAGCGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
805 CCAGCCTCCTGG
ClaI 540 ATCGAT AT^CGAT
1954 ATCGAT
DpnII 653 GATC ^GATC
971 GATC
EagI 847 CGGCCG C^GGCCG
974 CGGCCG
EclXI 847 CGGCCG C^GGCCG
974 CGGCCG
Eco52I 847 CGGCCG C^GGCCG
974 CGGCCG
MboI 653 GATC ^GATC
971 GATC
MluNI 535 TGGCCA TGG^CCA
1936 TGGCCA
MscI 535 TGGCCA TGG^CCA
1936 TGGCCA
NdeII 653 GATC ^GATC
971 GATC
NspI 487 GCATGC [GA]CATG^[CT]
2056 ACATGC
Sau3AI 653 GATC ^GATC
971 GATC
StuI 400 AGGCCT AGG^CCT
1292 AGGCCT
XcmI 664 CCATCCTCTGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1811 CCAAGGAATTATTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 88 enzymes) #
#########################################
AccII AciI AcyI AluI ApaI
AspHI AvaI BanI BanII BbvI
BcnI BglI BmyI BpmI BsaHI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmFI BsoBI Bsp1286I BspHI
BspMI BsrBI BsrFI BsrI BssKI
BstF5I BstNI BstUI Cac8I CfoI
Cfr10I Cfr13I DdeI DraII DsaI
EaeI EcoNI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeIII HapII
HgaI HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI MaeII MaeIII
MboII MnlI MseI MslI MspA1I
MspI MvaI MvnI MwoI NaeI
NciI NcoI NgoMIV NlaIII NlaIV
PleI PvuII RcaI Sau96I ScrFI
SfaNI StyI TaqI Tru9I TseI
Tsp45I TspRI TthHB8I