##################################
# NO CUT ENZYMES ( 100 enzymes) #
##################################
AatII AccI AclI AflIII AgeI
AhdI Alw44I AocI Aor51HI ApaLI
AscI AseI AsnI AspEI AspI
AviII AvrII BalI BbrPI BbsI
BciVI BclI BglI BlnI BpuAI
BsaAI BsiEI BsiWI BsmBI Bsp1407I
BspDI BspLU11I BsrGI BssHII BssSI
Bst1107I BstBI BstZ17I Bsu36I ClaI
CpoI DraI DrdI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoRI
EcoRV FbaI FseI FspI HincII
HindII HpaI KspI MfeI MluI
MluNI MscI MunI NaeI NdeI
NgoMIV NheI NotI NruI NspV
PacI PflFI PinAI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PvuI RsrII SacII SalI SbfI
ScaI SfiI SfuI SgrAI SmaI
SnaBI SpeI Sse8387I SspBI SspI
SwaI Tth111I XbaI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 49 enzymes) #
###################################
Acc65I 1125 GGTACC G^GTACC
AccIII 1450 TCCGGA T^CCGGA
AflII 2111 CTTAAG C^TTAAG
Asp700I 1758 GAACAGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 1125 GGTACC G^GTACC
AvaII 1289 GGACC G^G[AT]CC
BamHI 1915 GGATCC G^GATCC
BbeI 57 GGCGCC GGCGC^C
BfrI 2111 CTTAAG C^TTAAG
BglII 1436 AGATCT A^GATCT
BlpI 210 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 210 GCTCAGC GC^T[ACGT]AGC
BsaBI 974 GATGGGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 465 GAGACC GGTCTC(1/5)
BsaWI 1450 TCCGGA [AT]^CCGG[AT]
BseAI 1450 TCCGGA T^CCGGA
BseRI 462 GAGGAG GAGGAG(10/8)
BspEI 1450 TCCGGA T^CCGGA
BspHI 1402 TCATGA T^CATGA
BspMI 1122 GCAGGT ACCTGC(4/8)
BsrDI 1142 CATTGC GCAATG(2/0)
BsrFI 269 ACCGGC [GA]^CCGG[CT]
BstAPI 2173 GCACTTACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 2018 GGTCACC G^GT[ACGT]ACC
BstPI 2018 GGTCACC G^GT[ACGT]ACC
CelII 210 GCTCAGC GC^T[ACGT]AGC
Cfr10I 269 ACCGGC [GA]^CCGG[CT]
DraIII 1889 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG
EarI 363 GAAGAG CTCTTC(1/4)
EcoNI 1841 CCTGAGTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 2018 GGTCACC G^GT[ACGT]ACC
HindIII 816 AAGCTT A^AGCTT
KasI 57 GGCGCC G^GCGCC
KpnI 1125 GGTACC GGTAC^C
Ksp632I 363 GAAGAG CTCTTC(1/4)
MaeII 1039 ACGT A^CGT
MamI 974 GATGGGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 1450 TCCGGA T^CCGGA
NarI 57 GGCGCC GG^CGCC
NspI 580 GCATGC [GA]CATG^[CT]
PflMI 1379 CCAAATCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
RcaI 1402 TCATGA T^CATGA
SapI 363 GAAGAGC GCTCTTC(1/4)
SexAI 1890 ACCTGGT A^CC[AT]GGT
SfoI 57 GGCGCC GGC^GCC
SphI 580 GCATGC GCATG^C
StuI 1993 AGGCCT AGG^CCT
Van91I 1379 CCAAATCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI 1758 GAACAGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AcsI 1192 AAATTC [GA]^AATT[CT]
2198 AAATTT
AcyI 25 GACGCC G[GA]^CG[CT]C
57 GGCGCC
ApaI 613 GGGCCC GGGCC^C
957 GGGCCC
ApoI 1192 AAATTC [GA]^AATT[CT]
2198 AAATTT
BbvCI 119 GCTGAGG CCTCAGC(-5/-2)
1601 GCTGAGG
BcgI 1796 CGACCTCTATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
80 GCAGGCGGCTCG
BsaHI 25 GACGCC G[GA]^CG[CT]C
57 GGCGCC
BsgI 4 GTGCAG GTGCAG(16/14)
19 GTGCAG
BsmI 1371 GAATGC GAATGC(1/-1)
1565 GAATGC
BsrBI 208 CCGCTC CCGCTC(-3/-3)
2004 GAGCGG
CfoI 58 GCGC GCG^C
153 GCGC
EcoT22I 582 ATGCAT ATGCA^T
1373 ATGCAT
HaeII 57 GGCGCC [GA]GCGC^[CT]
152 GGCGCT
HhaI 58 GCGC GCG^C
153 GCGC
Hin1I 25 GACGCC G[GA]^CG[CT]C
57 GGCGCC
HinP1I 58 GCGC G^CGC
153 GCGC
MseI 382 TTAA T^TAA
2112 TTAA
MslI 1709 CACTGTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1808 CATGGTGGTG
NsiI 582 ATGCAT ATGCA^T
1373 ATGCAT
PaeR7I 46 CTCGAG C^TCGAG
1823 CTCGAG
PvuII 213 CAGCTG CAG^CTG
1599 CAGCTG
Tru9I 382 TTAA T^TAA
2112 TTAA
Tsp509I 1193 AATT ^AATT
2199 AATT
XcmI 1261 CCATGGCCTACATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1990 CCAAGGCCTTGGTGG
XhoI 46 CTCGAG C^TCGAG
1823 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 84 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AlwNI AspHI AvaI BanI BanII
BbvI BcnI BfaI BmyI BpmI
BsaJI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BsrI BssKI
BstF5I BstNI BstUI BstXI BstYI
Cac8I Cfr13I DdeI DpnII DraII
DsaI EaeI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeIII HapII
HgaI HinfI HpaII HphI ItaI
MaeI MaeIII MboI MboII MflI
MnlI MspA1I MspI MvaI MvnI
MwoI NciI NcoI NdeII NlaIII
NlaIV PleI PstI RsaI SacI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI StyI TaqI TfiI TseI
Tsp45I TspRI TthHB8I XhoII