##################################
# NO CUT ENZYMES ( 120 enzymes) #
##################################
AatII Acc65I AccIII AclI AcsI
AflII AgeI AhdI Alw44I AlwI
AocI Aor51HI ApaLI ApoI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI AviII AvrII BamHI BbeI
BbrPI BbsI BclI BfrI BlnI
BpuAI BsaAI BseAI BsmBI BsmI
BspDI BspEI BspHI BspLU11I BsrBI
BsrDI BsrFI Bst1107I BstBI BstXI
BstZ17I Bsu36I Cfr10I ClaI CpoI
DraI DrdI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoNI EcoRI
EcoRV EcoT14I EcoT22I FbaI FseI
FspI HaeII HindIII HpaI KasI
KpnI MfeI MluI MroI MunI
NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspI
NspV PacI PflFI PflMI PinAI
PmaCI PmeI PmlI PshBI Psp1406I
PvuI RcaI RsrII SalI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I StyI SwaI Tth111I Van91I
XbaI XcmI XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 44 enzymes) #
###################################
AccI 279 GTAGAC GT^[AC][GT]AC
AflIII 1092 ACACGT A^C[GA][CT]GT
ApaI 199 GGGCCC GGGCC^C
BalI 1255 TGGCCA TGG^CCA
BanI 1212 GGCACC G^G[CT][GA]CC
BcgI 240 CGAGAACTCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 1545 GGATAC GTATCC(6/5)
BglI 365 GCCAGCATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 250 GCTAAGC GC^T[ACGT]AGC
Bpu1102I 250 GCTAAGC GC^T[ACGT]AGC
BsaBI 928 GATGTACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 67 TCCGGT [AT]^CCGG[AT]
BseRI 304 CTCCTC GAGGAG(10/8)
BsiEI 236 CGACCG CG[GA][CT]^CG
BsiWI 191 CGTACG C^GTACG
BspMI 2 GCAGGT ACCTGC(4/8)
BssHII 150 GCGCGC G^CGCGC
BssSI 1125 CTCGTG CACGAG(-5/-1)
BstEII 1517 GGTCACC G^GT[ACGT]ACC
BstF5I 489 GGATG GGATG(2/0)
BstPI 1517 GGTCACC G^GT[ACGT]ACC
CelII 250 GCTAAGC GC^T[ACGT]AGC
DraII 921 AGGTCCT [GA]G^G[ACGT]CC[CT]
DraIII 1214 CACCAAGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 30 CCGCGG C^C[GA][CT]GG
EaeI 1255 TGGCCA [CT]^GGCC[GA]
EcoO109I 921 AGGTCCT [GA]G^G[ACGT]CC[CT]
EcoO65I 1517 GGTCACC G^GT[ACGT]ACC
FokI 489 GGATG GGATG(9/13)
HincII 1270 GTTGAC GT[CT]^[GA]AC
HindII 1270 GTTGAC GT[CT]^[GA]AC
KspI 30 CCGCGG CCGC^GG
MamI 928 GATGTACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 1255 TGGCCA TGG^CCA
MscI 1255 TGGCCA TGG^CCA
MslI 901 CATATCAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
PpuMI 921 AGGTCCT [GA]G^G[AT]CC[CT]
PshAI 1588 GACTGCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuII 13 CAGCTG CAG^CTG
SacII 30 CCGCGG CCGC^GG
SapI 1283 GAAGAGC GCTCTTC(1/4)
SfaNI 1334 GATGC GCATC(5/9)
SspI 1224 AATATT AAT^ATT
StuI 1673 AGGCCT AGG^CCT
###################################
# TWO CUT ENZYMES ( 16 enzymes) #
###################################
AlwNI 375 CAGAAGCTG CAG[ACGT][ACGT][ACGT]^CTG
1066 CAGAAGCTG
AvaII 922 GGTCC G^G[AT]CC
964 GGACC
BbvCI 15 GCTGAGG CCTCAGC(-5/-2)
380 GCTGAGG
BglII 451 AGATCT A^GATCT
811 AGATCT
BsgI 319 GTGCAG GTGCAG(16/14)
997 GTGCAG
BstAPI 205 GCAGTTATTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1592 GCAGTCAGTGC
BstYI 451 AGATCT [GA]^GATC[CT]
811 AGATCT
EarI 629 CTCTTC CTCTTC(1/4)
1283 GAAGAG
Ksp632I 629 CTCTTC CTCTTC(1/4)
1283 GAAGAG
MaeII 1094 ACGT A^CGT
1306 ACGT
MflI 451 AGATCT [GA]^GATC[CT]
811 AGATCT
PaeR7I 343 CTCGAG C^TCGAG
621 CTCGAG
PleI 123 GAGTC GAGTC(4/5)
851 GAGTC
SacI 480 GAGCTC GAGCT^C
663 GAGCTC
XhoI 343 CTCGAG C^TCGAG
621 CTCGAG
XhoII 451 AGATCT [GA]^GATC[CT]
811 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 78 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AspHI AvaI BanII BbvI BcnI
BfaI BmyI BpmI BsaHI BsaI
BsaJI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I Bsp1407I BsrGI
BsrI BssKI BstNI BstUI Cac8I
CfoI Cfr13I DdeI DpnII EcoRII
FauI Fnu4HI HaeIII HapII HgaI
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI MaeI MaeIII MboI
MboII MnlI MseI MspA1I MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV PstI RsaI Sau3AI
Sau96I ScrFI SfcI SmlI SspBI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I