##################################
# NO CUT ENZYMES ( 120 enzymes) #
##################################
Acc65I AccIII AclI AflII AgeI
AhdI Alw44I AocI Aor51HI ApaI
ApaLI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AviII AvrII
BamHI BbrPI BbvCI BcgI BciVI
BclI BfrI BglII BlnI BlpI
BpmI Bpu1102I BsaBI BseAI BsiEI
BsiWI BsmBI BsmI Bsp1407I BspDI
BspEI BspHI BspLU11I BsrBI BsrGI
BssSI Bst1107I BstBI BstZ17I Bsu36I
CelII ClaI CpoI DraIII DrdI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoNI EcoRV EcoT22I FbaI
FseI FspI HincII HindII HindIII
HpaI KpnI KspI MamI MfeI
MluI MroI MunI NcoI NdeI
NheI NotI NruI NsiI NspI
NspV PacI PflFI PinAI PmaCI
PmeI PmlI PshBI Psp1406I PstI
PvuI RcaI RsrII SacII SalI
SbfI ScaI SexAI SfcI SfiI
SfuI SmaI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI SwaI
Tth111I XcmI XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 31 enzymes) #
###################################
AatII 649 GACGTC GACGT^C
AccI 323 GTCTAC GT^[AC][GT]AC
AflIII 1227 ACGTGT A^C[GA][CT]GT
AspHI 452 GAGCTC G[AT]GC[AT]^C
BbsI 742 GTCTTC GAAGAC(2/6)
BpuAI 742 GTCTTC GAAGAC(2/6)
BsaAI 1268 TACGTG [CT]AC^GT[GA]
BsaWI 300 ACCGGA [AT]^CCGG[AT]
BsgI 1271 GTGCAG GTGCAG(16/14)
BsiHKAI 452 GAGCTC G[AT]GC[AT]^C
BsrDI 378 GCAATG GCAATG(2/0)
BssHII 80 GCGCGC G^CGCGC
BstEII 1168 GGTGACC G^GT[ACGT]ACC
BstPI 1168 GGTGACC G^GT[ACGT]ACC
BstYI 1879 AGATCC [GA]^GATC[CT]
EcoO65I 1168 GGTGACC G^GT[ACGT]ACC
EcoRI 507 GAATTC G^AATTC
MflI 1879 AGATCC [GA]^GATC[CT]
PaeR7I 1204 CTCGAG C^TCGAG
PflMI 719 CCAGGGGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 1416 AGGTCCT [GA]G^G[AT]CC[CT]
PshAI 1335 GACCCGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuII 461 CAGCTG CAG^CTG
SacI 452 GAGCTC GAGCT^C
SapI 1240 GCTCTTC GCTCTTC(1/4)
SgrAI 41 CACCGGCG C[GA]^CCGG[CT]G
SmlI 1204 CTCGAG C^T[CT][GA]AG
Van91I 719 CCAGGGGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 364 TCTAGA T^CTAGA
XhoI 1204 CTCGAG C^TCGAG
XhoII 1879 AGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 17 enzymes) #
###################################
AvaII 540 GGACC G^G[AT]CC
1417 GGTCC
BalI 525 TGGCCA TGG^CCA
1281 TGGCCA
BfaI 234 CTAG C^TAG
365 CTAG
BglI 48 GCCGCGTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
798 GCCATATTGGC
BsaI 1058 GAGACC GGTCTC(1/5)
1489 GGTCTC
BstAPI 1134 GCAGAAACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1414 GCAGGTCCTGC
BstXI 810 CCATGAGAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
973 CCAATCCAGTGG
DraI 1365 TTTAAA TTT^AAA
1544 TTTAAA
DraII 940 AGGCCCT [GA]G^G[ACGT]CC[CT]
1416 AGGTCCT
EcoO109I 940 AGGCCCT [GA]G^G[ACGT]CC[CT]
1416 AGGTCCT
FauI 1117 CCCGC CCCGC(4/6)
1765 CCCGC
MaeI 234 CTAG C^TAG
365 CTAG
MluNI 525 TGGCCA TGG^CCA
1281 TGGCCA
MscI 525 TGGCCA TGG^CCA
1281 TGGCCA
NaeI 374 GCCGGC GCC^GGC
1423 GCCGGC
NgoMIV 374 GCCGGC G^CCGGC
1423 GCCGGC
TfiI 729 GATTC G^A[AT]TC
1812 GATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 90 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AluI AlwI AlwNI ApoI AvaI
BanI BanII BbeI BbvI BcnI
BmyI BsaHI BsaJI BseRI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BspMI BsrFI BsrI BssKI BstF5I
BstNI BstUI Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DsaI EaeI
EarI EcoRII EcoT14I Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI KasI Ksp632I MaeII MaeIII
MboI MboII MnlI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NarI NciI NdeII NlaIII NlaIV
PleI RsaI Sau3AI Sau96I ScrFI
SfaNI SfoI StyI TaqI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I