##################################
# NO CUT ENZYMES ( 136 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcyI AgeI AhdI Alw44I
Aor51HI ApaI ApaLI AscI Asp700I
Asp718I AspEI AspHI AspI AvaI
AvrII BalI BamHI BanI BanII
BbeI BbrPI BbvCI BcgI BclI
BcnI BglI BlnI BlpI BmyI
Bpu1102I BsaBI BsaHI BsaWI BseAI
BsgI BsiEI BsiHKAI BsiWI BsoBI
Bsp1286I Bsp1407I BspDI BspEI BsrBI
BsrFI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstUI BstXI BstZ17I
CelII Cfr10I ClaI CpoI DsaI
EaeI EagI Eam1105I EclXI Eco47III
Eco52I EcoRI EcoRV FauI FbaI
FseI HaeII HapII HgaI Hin1I
HpaI HpaII KasI KpnI KspI
MamI MfeI MluI MluNI MroI
MscI MspI MunI MvnI NaeI
NarI NciI NcoI NgoMIV NheI
NotI NruI NspV PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PshAI Psp1406I PvuI RsrII
SacI SacII SalI SbfI SfiI
SfoI SfuI SgrAI SmaI SpeI
Sse8387I SspBI SwaI Tth111I Van91I
XbaI XcmI XhoI XmaCI XmaI
XmnI
###################################
# ONE CUT ENZYMES ( 43 enzymes) #
###################################
AflII 1251 CTTAAG C^TTAAG
AflIII 1436 ACATGT A^C[GA][CT]GT
AlwNI 1151 CAGAGCCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 945 CCTGAGG CC^T[ACGT]AGG
AseI 1093 ATTAAT AT^TAAT
AsnI 1093 ATTAAT AT^TAAT
AvaII 356 GGACC G^G[AT]CC
AviII 456 TGCGCA TGC^GCA
BfrI 1251 CTTAAG C^TTAAG
BsaAI 1341 TACGTA [CT]AC^GT[GA]
BsaI 734 GGTCTC GGTCTC(1/5)
BseRI 687 GAGGAG GAGGAG(10/8)
BsiYI 542 CCTGACCAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 542 CCTGACCAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmBI 184 GAGACG CGTCTC(1/5)
BsmFI 934 GTCCC GGGAC(10/14)
BsmI 723 GAATGC GAATGC(1/-1)
BspLU11I 1436 ACATGT A^CATGT
BspMI 1916 GCAGGT ACCTGC(4/8)
Bsu36I 945 CCTGAGG CC^T[ACGT]AGG
CfoI 457 GCGC GCG^C
DraII 355 AGGACCC [GA]G^G[ACGT]CC[CT]
DraIII 1019 CACTTAGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 1435 GACATGTTTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 945 CCTGAGG CC^T[ACGT]AGG
EcoNI 542 CCTGACCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 355 AGGACCC [GA]G^G[ACGT]CC[CT]
EcoT14I 547 CCAAGG C^C[AT][AT]GG
FspI 456 TGCGCA TGC^GCA
HhaI 457 GCGC GCG^C
HinP1I 457 GCGC G^CGC
MslI 531 CACATAAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 57 CAGCTG C[AC]G^C[GT]G
PpuMI 355 AGGACCC [GA]G^G[AT]CC[CT]
PshBI 1093 ATTAAT AT^TAAT
PvuII 57 CAGCTG CAG^CTG
SapI 1175 GAAGAGC GCTCTTC(1/4)
ScaI 762 AGTACT AGT^ACT
SexAI 1215 ACCTGGT A^CC[AT]GGT
SmlI 1251 CTTAAG C^T[CT][GA]AG
SnaBI 1341 TACGTA TAC^GTA
SphI 2194 GCATGC GCATG^C
StyI 547 CCAAGG C^C[AT][AT]GG
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AciI 744 CCGC CCGC(-3/-1)
1677 CCGC
BglII 1482 AGATCT A^GATCT
1638 AGATCT
BpmI 1009 CTGGAG CTGGAG(16/14)
1634 CTGGAG
BsaJI 547 CCAAGG C^C[ACGT][ACGT]GG
867 CCGAGG
BspHI 810 TCATGA T^CATGA
1311 TCATGA
BsrDI 146 CATTGC GCAATG(2/0)
1199 GCAATG
BstEII 635 GGTCACC G^GT[ACGT]ACC
1513 GGTTACC
BstPI 635 GGTCACC G^GT[ACGT]ACC
1513 GGTTACC
Cfr13I 356 GGACC G^G[ACGT]CC
2229 GGGCC
DraI 1657 TTTAAA TTT^AAA
2146 TTTAAA
EcoO65I 635 GGTCACC G^GT[ACGT]ACC
1513 GGTTACC
EcoT22I 244 ATGCAT ATGCA^T
2196 ATGCAT
HindIII 236 AAGCTT A^AGCTT
2313 AAGCTT
HphI 637 TCACC GGTGA(8/7)
988 TCACC
MaeII 1281 ACGT A^CGT
1342 ACGT
NsiI 244 ATGCAT ATGCA^T
2196 ATGCAT
PleI 263 GAGTC GAGTC(4/5)
1778 GAGTC
PstI 54 CTGCAG CTGCA^G
1148 CTGCAG
RcaI 810 TCATGA T^CATGA
1311 TCATGA
Sau96I 356 GGACC G^G[ACGT]CC
2229 GGGCC
SfaNI 246 GCATC GCATC(5/9)
471 GCATC
StuI 949 AGGCCT AGG^CCT
1005 AGGCCT
TfiI 836 GATTC G^A[AT]TC
2166 GATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 56 enzymes) #
#########################################
AcsI AfaI AluI AlwI ApoI
BbsI BbvI BciVI BfaI BpuAI
BsmAI BsrI BssKI BstF5I BstNI
BstYI Cac8I DdeI DpnII EarI
EcoRII Fnu4HI FokI HaeIII HincII
HindII HinfI ItaI Ksp632I MaeI
MaeIII MboI MboII MflI MnlI
MseI MvaI MwoI NdeI NdeII
NlaIII NlaIV NspI RsaI Sau3AI
ScrFI SfcI SspI TaqI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XhoII