##################################
# NO CUT ENZYMES ( 139 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcyI AflII AflIII AgeI AhdI
Alw44I AlwNI AocI Aor51HI ApaI
ApaLI AscI AseI AsnI Asp718I
AspEI AspI AvaI AvrII BalI
BamHI BanI BbeI BbrPI BciVI
BclI BfrI BglI BglII BlnI
BlpI BpmI Bpu1102I BsaAI BsaHI
BsaI BsaWI BseAI BsiWI BsoBI
BspDI BspEI BspHI BspLU11I BspMI
BsrBI BsrFI BssHII BstBI BstEII
BstPI BstUI BstXI BstYI Bsu36I
CelII Cfr10I ClaI CpoI DraI
DraIII EaeI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoO65I EcoRI
EcoRV FbaI FseI HaeII HgaI
Hin1I HincII HindII HpaI KasI
KpnI KspI MfeI MflI MluI
MluNI MroI MscI MunI MvnI
NaeI NarI NgoMIV NotI NruI
NspV PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SacII SalI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspI
SwaI Tth111I Van91I XbaI XcmI
XhoI XhoII XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 45 enzymes) #
###################################
AccI 1027 GTATAC GT^[AC][GT]AC
AspHI 968 GAGCTC G[AT]GC[AT]^C
AviII 1074 TGCGCA TGC^GCA
BbvCI 1219 GCTGAGG CCTCAGC(-5/-2)
BcgI 613 CGAGGTCTTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BsaBI 1351 GATTTTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsgI 915 CTGCAC GTGCAG(16/14)
BsiEI 5 CGACCG CG[GA][CT]^CG
BsiHKAI 968 GAGCTC G[AT]GC[AT]^C
BsmBI 1540 CGTCTC CGTCTC(1/5)
Bsp1407I 800 TGTACA T^GTACA
BsrGI 800 TGTACA T^GTACA
BssSI 694 CACGAG CACGAG(-5/-1)
Bst1107I 1027 GTATAC GTA^TAC
BstZ17I 1027 GTATAC GTA^TAC
CfoI 1075 GCGC GCG^C
DraII 1319 AGGACCT [GA]G^G[ACGT]CC[CT]
DrdI 244 GACAGTTTTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 302 CCATGG C^C[GA][CT]GG
EcoNI 1323 CCTAGATGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 1319 AGGACCT [GA]G^G[ACGT]CC[CT]
EcoT14I 302 CCATGG C^C[AT][AT]GG
EcoT22I 74 ATGCAT ATGCA^T
FauI 1651 GCGGG CCCGC(4/6)
FspI 1074 TGCGCA TGC^GCA
HhaI 1075 GCGC GCG^C
HinP1I 1075 GCGC G^CGC
MaeII 1539 ACGT A^CGT
MamI 1351 GATTTTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MspA1I 1078 CAGCTG C[AC]G^C[GT]G
NcoI 302 CCATGG C^CATGG
NdeI 791 CATATG CA^TATG
NheI 151 GCTAGC G^CTAGC
NsiI 74 ATGCAT ATGCA^T
NspI 310 GCATGT [GA]CATG^[CT]
PleI 1299 GACTC GAGTC(4/5)
PpuMI 1319 AGGACCT [GA]G^G[AT]CC[CT]
PstI 714 CTGCAG CTGCA^G
PvuII 1078 CAGCTG CAG^CTG
SacI 968 GAGCTC GAGCT^C
SapI 965 GAAGAGC GCTCTTC(1/4)
SfcI 714 CTGCAG C^T[GA][CT]AG
SspBI 800 TGTACA T^GTACA
StyI 302 CCATGG C^C[AT][AT]GG
Tsp45I 566 GTGAC ^GT[GC]AC
###################################
# TWO CUT ENZYMES ( 15 enzymes) #
###################################
BanII 329 GAGCCC G[GA]GC[CT]^C
968 GAGCTC
BbsI 910 GAAGAC GAAGAC(2/6)
1627 GAAGAC
BcnI 201 CCGGG CC^[GC]GG
255 CCGGG
BpuAI 910 GAAGAC GAAGAC(2/6)
1627 GAAGAC
BsmFI 258 GGGAC GGGAC(10/14)
1314 GGGAC
BsrDI 708 CATTGC GCAATG(2/0)
526 GCAATG
BsrI 339 ACTGG ACTGG(1/-1)
1214 CCAGT
BstAPI 76 GCATACTATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
707 GCATTGCCTGC
HphI 1348 GGTGA GGTGA(8/7)
1521 TCACC
MaeIII 566 GTGAC ^GT[ACGT]AC
977 GTTAC
NciI 201 CCGGG CC^[GC]GG
255 CCGGG
SmlI 508 CTCAAG C^T[CT][GA]AG
960 CTTGAG
StuI 701 AGGCCT AGG^CCT
1265 AGGCCT
TaqI 23 TCGA T^CGA
168 TCGA
TthHB8I 23 TCGA T^CGA
168 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 59 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI Asp700I AvaII BbvI BfaI
BmyI BsaJI BseRI BsiYI BslI
BsmAI BsmI Bsp1286I BssKI BstF5I
BstNI Cac8I Cfr13I DdeI DpnII
EarI EcoRII Fnu4HI FokI HaeIII
HapII HindIII HinfI HpaII ItaI
Ksp632I MaeI MboI MboII MnlI
MseI MslI MspI MvaI MwoI
NdeII NlaIII NlaIV RsaI Sau3AI
Sau96I ScrFI SfaNI TfiI Tru9I
TseI Tsp509I TspRI XmnI