################################## # NO CUT ENZYMES ( 139 enzymes) # ################################## AatII Acc65I AccII AccIII AclI AcyI AflII AflIII AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp718I AspEI AspI AvaI AvrII BalI BamHI BanI BbeI BbrPI BciVI BclI BfrI BglI BglII BlnI BlpI BpmI Bpu1102I BsaAI BsaHI BsaI BsaWI BseAI BsiWI BsoBI BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrFI BssHII BstBI BstEII BstPI BstUI BstXI BstYI Bsu36I CelII Cfr10I ClaI CpoI DraI DraIII EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoO65I EcoRI EcoRV FbaI FseI HaeII HgaI Hin1I HincII HindII HpaI KasI KpnI KspI MfeI MflI MluI MluNI MroI MscI MunI MvnI NaeI NarI NgoMIV NotI NruI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RcaI RsrII SacII SalI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspI SwaI Tth111I Van91I XbaI XcmI XhoI XhoII XmaCI XmaI ################################### # ONE CUT ENZYMES ( 45 enzymes) # ################################### AccI 1027 GTATAC GT^[AC][GT]AC AspHI 968 GAGCTC G[AT]GC[AT]^C AviII 1074 TGCGCA TGC^GCA BbvCI 1219 GCTGAGG CCTCAGC(-5/-2) BcgI 613 CGAGGTCTTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BsaBI 1351 GATTTTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsgI 915 CTGCAC GTGCAG(16/14) BsiEI 5 CGACCG CG[GA][CT]^CG BsiHKAI 968 GAGCTC G[AT]GC[AT]^C BsmBI 1540 CGTCTC CGTCTC(1/5) Bsp1407I 800 TGTACA T^GTACA BsrGI 800 TGTACA T^GTACA BssSI 694 CACGAG CACGAG(-5/-1) Bst1107I 1027 GTATAC GTA^TAC BstZ17I 1027 GTATAC GTA^TAC CfoI 1075 GCGC GCG^C DraII 1319 AGGACCT [GA]G^G[ACGT]CC[CT] DrdI 244 GACAGTTTTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC DsaI 302 CCATGG C^C[GA][CT]GG EcoNI 1323 CCTAGATGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO109I 1319 AGGACCT [GA]G^G[ACGT]CC[CT] EcoT14I 302 CCATGG C^C[AT][AT]GG EcoT22I 74 ATGCAT ATGCA^T FauI 1651 GCGGG CCCGC(4/6) FspI 1074 TGCGCA TGC^GCA HhaI 1075 GCGC GCG^C HinP1I 1075 GCGC G^CGC MaeII 1539 ACGT A^CGT MamI 1351 GATTTTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MspA1I 1078 CAGCTG C[AC]G^C[GT]G NcoI 302 CCATGG C^CATGG NdeI 791 CATATG CA^TATG NheI 151 GCTAGC G^CTAGC NsiI 74 ATGCAT ATGCA^T NspI 310 GCATGT [GA]CATG^[CT] PleI 1299 GACTC GAGTC(4/5) PpuMI 1319 AGGACCT [GA]G^G[AT]CC[CT] PstI 714 CTGCAG CTGCA^G PvuII 1078 CAGCTG CAG^CTG SacI 968 GAGCTC GAGCT^C SapI 965 GAAGAGC GCTCTTC(1/4) SfcI 714 CTGCAG C^T[GA][CT]AG SspBI 800 TGTACA T^GTACA StyI 302 CCATGG C^C[AT][AT]GG Tsp45I 566 GTGAC ^GT[GC]AC ################################### # TWO CUT ENZYMES ( 15 enzymes) # ################################### BanII 329 GAGCCC G[GA]GC[CT]^C 968 GAGCTC BbsI 910 GAAGAC GAAGAC(2/6) 1627 GAAGAC BcnI 201 CCGGG CC^[GC]GG 255 CCGGG BpuAI 910 GAAGAC GAAGAC(2/6) 1627 GAAGAC BsmFI 258 GGGAC GGGAC(10/14) 1314 GGGAC BsrDI 708 CATTGC GCAATG(2/0) 526 GCAATG BsrI 339 ACTGG ACTGG(1/-1) 1214 CCAGT BstAPI 76 GCATACTATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 707 GCATTGCCTGC HphI 1348 GGTGA GGTGA(8/7) 1521 TCACC MaeIII 566 GTGAC ^GT[ACGT]AC 977 GTTAC NciI 201 CCGGG CC^[GC]GG 255 CCGGG SmlI 508 CTCAAG C^T[CT][GA]AG 960 CTTGAG StuI 701 AGGCCT AGG^CCT 1265 AGGCCT TaqI 23 TCGA T^CGA 168 TCGA TthHB8I 23 TCGA T^CGA 168 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 59 enzymes) # ######################################### AciI AcsI AfaI AluI AlwI ApoI Asp700I AvaII BbvI BfaI BmyI BsaJI BseRI BsiYI BslI BsmAI BsmI Bsp1286I BssKI BstF5I BstNI Cac8I Cfr13I DdeI DpnII EarI EcoRII Fnu4HI FokI HaeIII HapII HindIII HinfI HpaII ItaI Ksp632I MaeI MboI MboII MnlI MseI MslI MspI MvaI MwoI NdeII NlaIII NlaIV RsaI Sau3AI Sau96I ScrFI SfaNI TfiI Tru9I TseI Tsp509I TspRI XmnI