##################################
# NO CUT ENZYMES ( 98 enzymes) #
##################################
AatII Acc65I AccIII AclI AflII
AflIII AgeI AhdI AlwNI Aor51HI
ApaI AscI AseI AsnI Asp700I
Asp718I AspEI AvrII BamHI BanII
BbrPI BbvCI BciVI BclI BfrI
BglII BlnI BlpI Bpu1102I BsaAI
BseAI BsgI BsiWI BsmBI BsmI
BspDI BspEI BspLU11I BspMI BssHII
BssSI Bst1107I BstBI BstXI BstZ17I
CelII ClaI CpoI Eam1105I Eco47III
EcoNI EcoRI FbaI FseI HindIII
HpaI KpnI KspI MfeI MluI
MroI MunI NdeI NheI NotI
NruI NspV PacI PaeR7I PflMI
PinAI PmaCI PmeI PmlI PshBI
Psp1406I PstI PvuI PvuII RsrII
SacI SacII SbfI SexAI SfiI
SfuI SgrAI SnaBI SpeI SphI
Sse8387I SspI StuI SwaI Van91I
XbaI XhoI XmnI
###################################
# ONE CUT ENZYMES ( 70 enzymes) #
###################################
AccI 93 GTCGAC GT^[AC][GT]AC
AcsI 1589 AAATTT [GA]^AATT[CT]
AcyI 172 GGCGCC G[GA]^CG[CT]C
Alw44I 1776 GTGCAC G^TGCAC
AocI 837 CCTGAGG CC^T[ACGT]AGG
ApaLI 1776 GTGCAC G^TGCAC
ApoI 1589 AAATTT [GA]^AATT[CT]
AspI 534 GACGGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 1243 TGCGCA TGC^GCA
BalI 745 TGGCCA TGG^CCA
BbeI 172 GGCGCC GGCGC^C
BbsI 153 GTCTTC GAAGAC(2/6)
BfaI 1190 CTAG C^TAG
BglI 141 GCCCCCCGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpmI 771 CTGGAG CTGGAG(16/14)
BpuAI 153 GTCTTC GAAGAC(2/6)
BsaHI 172 GGCGCC G[GA]^CG[CT]C
BsaWI 528 TCCGGT [AT]^CCGG[AT]
BsiEI 673 CGGCCG CG[GA][CT]^CG
Bsp1407I 1745 TGTACA T^GTACA
BsrFI 120 GCCGGC [GA]^CCGG[CT]
BsrGI 1745 TGTACA T^GTACA
BstAPI 365 GCACCCCGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 539 GGTCACC G^GT[ACGT]ACC
BstPI 539 GGTCACC G^GT[ACGT]ACC
BstYI 637 AGATCC [GA]^GATC[CT]
Bsu36I 837 CCTGAGG CC^T[ACGT]AGG
Cfr10I 120 GCCGGC [GA]^CCGG[CT]
DraI 1333 TTTAAA TTT^AAA
DrdI 795 GACGGCCAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 673 CGGCCG C^GGCCG
EclXI 673 CGGCCG C^GGCCG
Eco52I 673 CGGCCG C^GGCCG
Eco81I 837 CCTGAGG CC^T[ACGT]AGG
EcoO65I 539 GGTCACC G^GT[ACGT]ACC
EcoRV 75 GATATC GAT^ATC
EcoT22I 1457 ATGCAT ATGCA^T
FspI 1243 TGCGCA TGC^GCA
HaeII 172 GGCGCC [GA]GCGC^[CT]
HgaI 593 GCGTC GACGC(5/10)
Hin1I 172 GGCGCC G[GA]^CG[CT]C
HincII 93 GTCGAC GT[CT]^[GA]AC
HindII 93 GTCGAC GT[CT]^[GA]AC
KasI 172 GGCGCC G^GCGCC
MaeI 1190 CTAG C^TAG
MflI 637 AGATCC [GA]^GATC[CT]
MluNI 745 TGGCCA TGG^CCA
MscI 745 TGGCCA TGG^CCA
MspA1I 830 CCGCTG C[AC]G^C[GT]G
NaeI 120 GCCGGC GCC^GGC
NarI 172 GGCGCC GG^CGCC
NcoI 64 CCATGG C^CATGG
NgoMIV 120 GCCGGC G^CCGGC
NsiI 1457 ATGCAT ATGCA^T
NspI 109 GCATGT [GA]CATG^[CT]
PflFI 534 GACGGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI 613 GGGACCT [GA]G^G[AT]CC[CT]
PshAI 1357 GACAGCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SalI 93 GTCGAC G^TCGAC
SapI 776 GAAGAGC GCTCTTC(1/4)
ScaI 1072 AGTACT AGT^ACT
SfcI 656 CTACAG C^T[GA][CT]AG
SfoI 172 GGCGCC GGC^GCC
SmaI 145 CCCGGG CCC^GGG
SspBI 1745 TGTACA T^GTACA
TfiI 216 GATTC G^A[AT]TC
Tth111I 534 GACGGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoII 637 AGATCC [GA]^GATC[CT]
XmaCI 145 CCCGGG C^CCGGG
XmaI 145 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AvaI 145 CCCGGG C^[CT]CG[GA]G
357 CCCGAG
AvaII 599 GGACC G^G[AT]CC
614 GGACC
BcgI 563 CGAGGGGTATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1559 GCATTGCTTTCG
BsaBI 1043 GATCAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1127 GATGTGGATC
BsoBI 145 CCCGGG C^[CT]CG[GA]G
357 CCCGAG
BspHI 631 TCATGA T^CATGA
910 TCATGA
BsrBI 678 GAGCGG CCGCTC(-3/-3)
822 GAGCGG
DraII 385 AGGCCCC [GA]G^G[ACGT]CC[CT]
613 GGGACCT
EarI 846 CTCTTC CTCTTC(1/4)
776 GAAGAG
EcoO109I 385 AGGCCCC [GA]G^G[ACGT]CC[CT]
613 GGGACCT
EcoT14I 64 CCATGG C^C[AT][AT]GG
400 CCAAGG
Ksp632I 846 CTCTTC CTCTTC(1/4)
776 GAAGAG
MamI 1043 GATCAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1127 GATGTGGATC
MslI 515 CATCGTGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
971 CACCACCATG
RcaI 631 TCATGA T^CATGA
910 TCATGA
SmlI 1684 CTTGAG C^T[CT][GA]AG
1755 CTTGAG
StyI 64 CCATGG C^C[AT][AT]GG
400 CCAAGG
Tsp509I 1575 AATT ^AATT
1590 AATT
XcmI 586 CCATCCTGCGTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
760 CCAGCTCCTCCCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 71 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AspHI BanI BbvI BcnI BmyI
BsaI BsaJI BseRI BsiHKAI BsiYI
BslI BsmAI BsmFI Bsp1286I BsrDI
BsrI BssKI BstF5I BstNI BstUI
Cac8I CfoI Cfr13I DdeI DpnII
DraIII DsaI EaeI EcoRII FauI
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HinfI HpaII HphI ItaI
MaeII MaeIII MboI MboII MnlI
MseI MspI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV PleI
RsaI Sau3AI Sau96I ScrFI SfaNI
TaqI Tru9I TseI Tsp45I TspRI
TthHB8I