##################################
# NO CUT ENZYMES ( 112 enzymes) #
##################################
Acc65I AccI AccIII AclI AcsI
AflII AgeI AhdI Alw44I AocI
ApaLI ApoI AscI AseI AsnI
Asp718I AspEI AspI AvrII BamHI
BbeI BbrPI BbsI BciVI BclI
BfrI BlnI BlpI Bpu1102I BpuAI
BsaAI BsaBI BseAI BsiWI BsmBI
Bsp1407I BspEI BspHI BsrDI BsrGI
BssHII BssSI Bst1107I BstBI BstEII
BstPI BstZ17I Bsu36I CelII CpoI
DraI EagI Eam1105I EclXI Eco52I
Eco81I EcoO65I EcoRI EcoT14I EcoT22I
FbaI HgaI HincII HindII HindIII
HpaI KasI KpnI MamI MfeI
MluI MroI MunI NarI NcoI
NdeI NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SalI ScaI SexAI SfoI SfuI
SgrAI SmlI SnaBI SpeI SspBI
SspI StyI SwaI Tsp509I Tth111I
XbaI XhoI
###################################
# ONE CUT ENZYMES ( 37 enzymes) #
###################################
AatII 563 GACGTC GACGT^C
AcyI 563 GACGTC G[GA]^CG[CT]C
AflIII 1361 ACATGT A^C[GA][CT]GT
AviII 730 TGCGCA TGC^GCA
BbvCI 531 GCTGAGG CCTCAGC(-5/-2)
BcgI 1638 CGAACCACTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI 1463 CTAG C^TAG
BglII 908 AGATCT A^GATCT
BsaHI 563 GACGTC G[GA]^CG[CT]C
BsiEI 12 CGACCG CG[GA][CT]^CG
BspDI 966 ATCGAT AT^CGAT
BspLU11I 1361 ACATGT A^CATGT
BsrFI 1387 GCCGGC [GA]^CCGG[CT]
BstXI 424 CCAGGAAACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 908 AGATCT [GA]^GATC[CT]
Cfr10I 1387 GCCGGC [GA]^CCGG[CT]
ClaI 966 ATCGAT AT^CGAT
DraIII 708 CACAGCGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 1429 GACCGTGCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 1530 CCGCGG C^C[GA][CT]GG
EcoNI 1462 CCTAGCCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV 1041 GATATC GAT^ATC
FseI 1386 GGCCGGCC GGCCGG^CC
FspI 730 TGCGCA TGC^GCA
Hin1I 563 GACGTC G[GA]^CG[CT]C
KspI 1530 CCGCGG CCGC^GG
MaeI 1463 CTAG C^TAG
MflI 908 AGATCT [GA]^GATC[CT]
MseI 1883 TTAA T^TAA
MslI 1821 CACACGGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI 1387 GCCGGC GCC^GGC
NgoMIV 1387 GCCGGC G^CCGGC
SacII 1530 CCGCGG CCGC^GG
SbfI 1109 CCTGCAGG CCTGCA^GG
Sse8387I 1109 CCTGCAGG CCTGCA^GG
Tru9I 1883 TTAA T^TAA
XhoII 908 AGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AfaI 201 GTAC GT^AC
1142 GTAC
AlwI 1556 GATCC GGATC(4/5)
1180 GGATC
Aor51HI 280 AGCGCT AGC^GCT
346 AGCGCT
ApaI 45 GGGCCC GGGCC^C
643 GGGCCC
Asp700I 392 GAAGGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1723 GAACTGCTTC
BalI 949 TGGCCA TGG^CCA
1486 TGGCCA
BanI 133 GGCACC G^G[CT][GA]CC
1534 GGCACC
BsaWI 1019 TCCGGT [AT]^CCGG[AT]
1766 ACCGGA
BsmI 621 GAATGC GAATGC(1/-1)
895 GAATGC
BsrBI 70 CCGCTC CCGCTC(-3/-3)
882 GAGCGG
BstAPI 482 GCAGGCTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
707 GCACAGCGTGC
Eco47III 280 AGCGCT AGC^GCT
346 AGCGCT
MluNI 949 TGGCCA TGG^CCA
1486 TGGCCA
MscI 949 TGGCCA TGG^CCA
1486 TGGCCA
RsaI 201 GTAC GT^AC
1142 GTAC
SacI 303 GAGCTC GAGCT^C
1104 GAGCTC
SphI 733 GCATGC GCATG^C
1846 GCATGC
StuI 541 AGGCCT AGG^CCT
1598 AGGCCT
TfiI 296 GAATC G^A[AT]TC
335 GATTC
Tsp45I 561 GTGAC ^GT[GC]AC
1890 GTCAC
XcmI 424 CCAGGAAACTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1515 CCACCCAGGAGCTGG
XmnI 392 GAAGGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1723 GAACTGCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 87 enzymes) #
#########################################
AccII AciI AluI AlwNI AspHI
AvaI AvaII BanII BbvI BcnI
BglI BmyI BpmI BsaI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BspMI
BsrI BssKI BstF5I BstNI BstUI
Cac8I CfoI Cfr13I DdeI DpnII
DraII EaeI EarI EcoO109I EcoRII
FauI Fnu4HI FokI HaeII HaeIII
HapII HhaI HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeII MaeIII
MboI MboII MnlI MspA1I MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV NspI PflMI PleI
PstI PvuII SapI Sau3AI Sau96I
ScrFI SfaNI SfcI SfiI SmaI
TaqI TseI TspRI TthHB8I Van91I
XmaCI XmaI