##################################
# NO CUT ENZYMES ( 121 enzymes) #
##################################
AatII AccI AccIII AclI AcyI
AflII AflIII AgeI AhdI Aor51HI
AscI AseI AsnI AspEI AspI
AvaI BamHI BbeI BbrPI BbvCI
BcgI BfrI BglII BsaAI BsaHI
BsaWI BseAI BseRI BsgI BsiEI
BsiWI BsmBI BsoBI Bsp1407I BspDI
BspEI BspHI BspLU11I BsrFI BsrGI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstYI BstZ17I Cfr10I
ClaI CpoI DrdI EagI Eam1105I
EclXI Eco47III Eco52I EcoO65I EcoRV
FseI HaeII Hin1I HincII HindII
HindIII HpaI KasI KspI MfeI
MflI MluI MroI MunI NaeI
NarI NdeI NgoMIV NotI NruI
NspV PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PstI PvuI RcaI
RsrII SacI SacII SalI SbfI
ScaI SexAI SfcI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspBI SwaI Tth111I
Van91I XbaI XhoI XhoII XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 44 enzymes) #
###################################
Acc65I 303 GGTACC G^GTACC
Alw44I 595 GTGCAC G^TGCAC
AlwI 248 GGATC GGATC(4/5)
AocI 1615 CCTGAGG CC^T[ACGT]AGG
ApaI 440 GGGCCC GGGCC^C
ApaLI 595 GTGCAC G^TGCAC
Asp700I 987 GAAATCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 303 GGTACC G^GTACC
AspHI 595 GTGCAC G[AT]GC[AT]^C
AviII 1803 TGCGCA TGC^GCA
AvrII 1155 CCTAGG C^CTAGG
BbsI 1621 GAAGAC GAAGAC(2/6)
BciVI 315 GGATAC GTATCC(6/5)
BlnI 1155 CCTAGG C^CTAGG
BlpI 1372 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 1372 GCTCAGC GC^T[ACGT]AGC
BpuAI 1621 GAAGAC GAAGAC(2/6)
BsaBI 735 GATTGAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiHKAI 595 GTGCAC G[AT]GC[AT]^C
BsmI 1289 GCATTC GAATGC(1/-1)
BspMI 785 ACCTGC ACCTGC(4/8)
BsrDI 516 CATTGC GCAATG(2/0)
Bsu36I 1615 CCTGAGG CC^T[ACGT]AGG
CelII 1372 GCTCAGC GC^T[ACGT]AGC
DraI 1593 TTTAAA TTT^AAA
DraIII 580 CACTTCGTG CAC[ACGT][ACGT][ACGT]^GTG
EarI 13 CTCTTC CTCTTC(1/4)
Eco81I 1615 CCTGAGG CC^T[ACGT]AGG
EcoT22I 1172 ATGCAT ATGCA^T
FspI 1803 TGCGCA TGC^GCA
HgaI 4 GCGTC GACGC(5/10)
KpnI 303 GGTACC GGTAC^C
Ksp632I 13 CTCTTC CTCTTC(1/4)
MaeII 659 ACGT A^CGT
MamI 735 GATTGAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NheI 937 GCTAGC G^CTAGC
NsiI 1172 ATGCAT ATGCA^T
NspI 620 ACATGC [GA]CATG^[CT]
PpuMI 954 GGGTCCC [GA]G^G[AT]CC[CT]
SapI 12 GCTCTTC GCTCTTC(1/4)
SspI 751 AATATT AAT^ATT
StuI 692 AGGCCT AGG^CCT
TfiI 1517 GATTC G^A[AT]TC
XmnI 987 GAAATCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AlwNI 205 CAGTCCCTG CAG[ACGT][ACGT][ACGT]^CTG
1696 CAGGAGCTG
BalI 167 TGGCCA TGG^CCA
1412 TGGCCA
BanII 440 GGGCCC G[GA]GC[CT]^C
1391 GGGCTC
BclI 1489 TGATCA T^GATCA
1812 TGATCA
BpmI 536 CTGGAG CTGGAG(16/14)
997 CTGGAG
BsaI 380 GAGACC GGTCTC(1/5)
222 GGTCTC
BsrBI 10 CCGCTC CCGCTC(-3/-3)
414 GAGCGG
BstXI 188 CCAAAGTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
969 CCAGTTTGATGG
DraII 439 GGGGCCC [GA]G^G[ACGT]CC[CT]
954 GGGTCCC
EcoNI 258 CCTGGTGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1362 CCTCATCCAGG
EcoO109I 439 GGGGCCC [GA]G^G[ACGT]CC[CT]
954 GGGTCCC
EcoRI 929 GAATTC G^AATTC
1843 GAATTC
FbaI 1489 TGATCA T^GATCA
1812 TGATCA
MluNI 167 TGGCCA TGG^CCA
1412 TGGCCA
MscI 167 TGGCCA TGG^CCA
1412 TGGCCA
NcoI 38 CCATGG C^CATGG
1452 CCATGG
PvuII 625 CAGCTG CAG^CTG
1229 CAGCTG
TaqI 293 TCGA T^CGA
1182 TCGA
TthHB8I 293 TCGA T^CGA
1182 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 74 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
ApoI AvaII BanI BbvI BcnI
BfaI BglI BmyI BsaJI BsiYI
BslI BsmAI BsmFI Bsp1286I BsrI
BssKI BstF5I BstNI BstUI Cac8I
CfoI Cfr13I DdeI DpnII DsaI
EaeI EcoRII EcoT14I FauI Fnu4HI
FokI HaeIII HapII HhaI HinP1I
HinfI HpaII HphI ItaI MaeI
MaeIII MboI MboII MnlI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
PleI RsaI Sau3AI Sau96I ScrFI
SfaNI SmlI StyI Tru9I TseI
Tsp45I Tsp509I TspRI XcmI