##################################
# NO CUT ENZYMES ( 110 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AflII AflIII AgeI AhdI Alw44I
AlwNI ApaLI AscI AseI AsnI
Asp700I Asp718I AspEI AspHI AviII
AvrII BamHI BcgI BclI BfaI
BfrI BglII BlnI BlpI Bpu1102I
BsaBI BseAI BsiHKAI BsiWI BsmBI
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrGI BssHII Bst1107I BstAPI BstBI
BstEII BstPI BstXI BstZ17I CelII
ClaI CpoI DraI DraIII DrdI
Eam1105I EcoNI EcoO65I EcoRI EcoRV
FbaI FseI FspI HincII HindII
HpaI KpnI KspI MaeI MamI
MfeI MluI MroI MunI NdeI
NheI NotI NruI NspV PacI
PinAI PmeI PshAI PshBI Psp1406I
PvuI RcaI RsrII SacI SacII
SalI SapI SbfI SexAI SfiI
SfuI SgrAI SmaI SnaBI SpeI
Sse8387I SspBI SspI StuI SwaI
TfiI XbaI XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 41 enzymes) #
###################################
Aor51HI 79 AGCGCT AGC^GCT
AspI 212 GACTGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 412 CTCGAG C^[CT]CG[GA]G
BalI 118 TGGCCA TGG^CCA
BbrPI 1151 CACGTG CAC^GTG
BbvCI 56 GCTGAGG CCTCAGC(-5/-2)
BciVI 1110 GGATAC GTATCC(6/5)
BglI 1258 GCCAGTTTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaAI 1151 CACGTG [CT]AC^GT[GA]
BsaWI 737 ACCGGA [AT]^CCGG[AT]
BsiEI 1586 CGGCCG CG[GA][CT]^CG
BsmI 235 GCATTC GAATGC(1/-1)
BsoBI 412 CTCGAG C^[CT]CG[GA]G
BsrBI 504 GAGCGG CCGCTC(-3/-3)
BsrDI 24 CATTGC GCAATG(2/0)
BssSI 1050 CTCGTG CACGAG(-5/-1)
BstYI 1518 AGATCC [GA]^GATC[CT]
EagI 1586 CGGCCG C^GGCCG
EarI 1418 GAAGAG CTCTTC(1/4)
EclXI 1586 CGGCCG C^GGCCG
Eco47III 79 AGCGCT AGC^GCT
Eco52I 1586 CGGCCG C^GGCCG
EcoT22I 923 ATGCAT ATGCA^T
HindIII 546 AAGCTT A^AGCTT
Ksp632I 1418 GAAGAG CTCTTC(1/4)
MflI 1518 AGATCC [GA]^GATC[CT]
MluNI 118 TGGCCA TGG^CCA
MscI 118 TGGCCA TGG^CCA
NsiI 923 ATGCAT ATGCA^T
PaeR7I 412 CTCGAG C^TCGAG
PflFI 212 GACTGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 935 CCAGCTTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 1151 CACGTG CAC^GTG
PmlI 1151 CACGTG CAC^GTG
PvuII 135 CAGCTG CAG^CTG
ScaI 697 AGTACT AGT^ACT
SmlI 412 CTCGAG C^T[CT][GA]AG
Tth111I 212 GACTGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 935 CCAGCTTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 412 CTCGAG C^TCGAG
XhoII 1518 AGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AcsI 448 AAATTC [GA]^AATT[CT]
1026 AAATTC
AlwI 1519 GATCC GGATC(4/5)
527 GGATC
AocI 351 CCTGAGG CC^T[ACGT]AGG
1035 CCTAAGG
ApaI 111 GGGCCC GGGCC^C
257 GGGCCC
ApoI 448 AAATTC [GA]^AATT[CT]
1026 AAATTC
BbsI 1215 GAAGAC GAAGAC(2/6)
1280 GTCTTC
BpuAI 1215 GAAGAC GAAGAC(2/6)
1280 GTCTTC
BsaI 651 GAGACC GGTCTC(1/5)
1045 GAGACC
BsmAI 651 GAGAC GTCTC(1/5)
1045 GAGAC
BsrFI 300 GCCGGC [GA]^CCGG[CT]
1202 GCCGGC
Bsu36I 351 CCTGAGG CC^T[ACGT]AGG
1035 CCTAAGG
Cfr10I 300 GCCGGC [GA]^CCGG[CT]
1202 GCCGGC
Eco81I 351 CCTGAGG CC^T[ACGT]AGG
1035 CCTAAGG
MslI 1271 CATGCAGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1500 CACACAAGTG
NaeI 300 GCCGGC GCC^GGC
1202 GCCGGC
NcoI 115 CCATGG C^CATGG
265 CCATGG
NgoMIV 300 GCCGGC G^CCGGC
1202 GCCGGC
NspI 314 GCATGC [GA]CATG^[CT]
1270 GCATGC
PstI 277 CTGCAG CTGCA^G
309 CTGCAG
SfcI 277 CTGCAG C^T[GA][CT]AG
309 CTGCAG
SphI 314 GCATGC GCATG^C
1270 GCATGC
Tsp45I 1149 GTCAC ^GT[GC]AC
1573 GTGAC
TspRI 275 CACTG CA[GC]TG[ACGT][ACGT]^
1571 CAGTG
XcmI 397 CCACGGTTGTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
832 CCAACTCATTCGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 83 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AvaII BanI BanII BbeI BbvI
BcnI BmyI BpmI BsaHI BsaJI
BseRI BsgI BsiYI BslI BsmFI
Bsp1286I BspMI BsrI BssKI BstF5I
BstNI BstUI Cac8I CfoI Cfr13I
DdeI DpnII DraII DsaI EaeI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI KasI MaeII MaeIII
MboI MboII MnlI MseI MspA1I
MspI MvaI MvnI MwoI NarI
NciI NdeII NlaIII NlaIV PleI
PpuMI RsaI Sau3AI Sau96I ScrFI
SfaNI SfoI StyI TaqI Tru9I
TseI Tsp509I TthHB8I