##################################
# NO CUT ENZYMES ( 115 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcsI AgeI AhdI Aor51HI ApaI
ApoI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AvrII BbrPI
BcgI BciVI BlnI BlpI Bpu1102I
BsaWI BseAI BsiEI BsiWI BsmBI
BsmI Bsp1407I BspDI BspEI BsrBI
BsrFI BsrGI BssHII Bst1107I BstAPI
BstEII BstPI BstUI BstZ17I CelII
Cfr10I ClaI CpoI DraIII DsaI
EagI Eam1105I EclXI Eco47III Eco52I
EcoO65I EcoRI EcoRV EcoT22I FseI
HgaI HincII HindII HpaI KpnI
KspI MluI MroI MvnI NaeI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PstI PvuI
PvuII RsrII SacI SacII SalI
SbfI ScaI SexAI SfcI SfiI
SgrAI SmaI SpeI SphI Sse8387I
SspBI SwaI Tsp45I Tth111I XbaI
XcmI XhoI XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 63 enzymes) #
###################################
AccI 638 GTAGAC GT^[AC][GT]AC
AcyI 88 GGCGCC G[GA]^CG[CT]C
AflII 159 CTTAAG C^TTAAG
AflIII 887 ACATGT A^C[GA][CT]GT
Alw44I 1961 GTGCAC G^TGCAC
AlwNI 1536 CAGTCTCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 593 CCTCAGG CC^T[ACGT]AGG
ApaLI 1961 GTGCAC G^TGCAC
AspHI 1961 GTGCAC G[AT]GC[AT]^C
AvaI 670 CCCGAG C^[CT]CG[GA]G
AviII 1016 TGCGCA TGC^GCA
BanII 1312 GAGCCC G[GA]GC[CT]^C
BbeI 88 GGCGCC GGCGC^C
BbvCI 1637 CCTCAGC CCTCAGC(-5/-2)
BclI 543 TGATCA T^GATCA
BcnI 364 CCGGG CC^[GC]GG
BfrI 159 CTTAAG C^TTAAG
BglI 917 GCCATTGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpmI 17 CTCCAG CTGGAG(16/14)
BsaAI 1853 TACGTA [CT]AC^GT[GA]
BsaHI 88 GGCGCC G[GA]^CG[CT]C
BsaI 550 GAGACC GGTCTC(1/5)
BsgI 1445 GTGCAG GTGCAG(16/14)
BsiHKAI 1961 GTGCAC G[AT]GC[AT]^C
BsoBI 670 CCCGAG C^[CT]CG[GA]G
BspHI 546 TCATGA T^CATGA
BspLU11I 887 ACATGT A^CATGT
BsrDI 1544 GCAATG GCAATG(2/0)
BsrI 645 ACTGG ACTGG(1/-1)
BssSI 393 CTCGTG CACGAG(-5/-1)
BstBI 96 TTCGAA TT^CGAA
BstXI 1914 CCAAAACTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 593 CCTCAGG CC^T[ACGT]AGG
DraII 598 GGGGCCT [GA]G^G[ACGT]CC[CT]
DrdI 1529 GACTCAACAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 593 CCTCAGG CC^T[ACGT]AGG
EcoNI 1579 CCTAATGTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 598 GGGGCCT [GA]G^G[ACGT]CC[CT]
EcoT14I 957 CCAAGG C^C[AT][AT]GG
FbaI 543 TGATCA T^GATCA
FspI 1016 TGCGCA TGC^GCA
HapII 364 CCGG C^CGG
Hin1I 88 GGCGCC G[GA]^CG[CT]C
HindIII 1790 AAGCTT A^AGCTT
HpaII 364 CCGG C^CGG
KasI 88 GGCGCC G^GCGCC
MaeII 1854 ACGT A^CGT
MfeI 23 CAATTG C^AATTG
MspA1I 69 CAGCGG C[AC]G^C[GT]G
MspI 364 CCGG C^CGG
MunI 23 CAATTG C^AATTG
NarI 88 GGCGCC GG^CGCC
NciI 364 CCGGG CC^[GC]GG
NspV 96 TTCGAA TT^CGAA
PflMI 19 CCAGCAATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
RcaI 546 TCATGA T^CATGA
SapI 1868 GAAGAGC GCTCTTC(1/4)
SfoI 88 GGCGCC GGC^GCC
SfuI 96 TTCGAA TT^CGAA
SnaBI 1853 TACGTA TAC^GTA
StyI 957 CCAAGG C^C[AT][AT]GG
TfiI 840 GATTC G^A[AT]TC
Van91I 19 CCAGCAATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
BanI 88 GGCGCC G^G[CT][GA]CC
748 GGCACC
BbsI 494 GAAGAC GAAGAC(2/6)
512 GAAGAC
BglII 1237 AGATCT A^GATCT
1725 AGATCT
BmyI 1312 GAGCCC G[AGT]GC[ACT]^C
1961 GTGCAC
BpuAI 494 GAAGAC GAAGAC(2/6)
512 GAAGAC
BsaBI 538 GATGGTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
859 GATTTCCATC
BseRI 1266 CTCCTC GAGGAG(10/8)
1323 CTCCTC
BsmFI 1010 GGGAC GGGAC(10/14)
905 GTCCC
Bsp1286I 1312 GAGCCC G[AGT]GC[ACT]^C
1961 GTGCAC
BspMI 483 ACCTGC ACCTGC(4/8)
1149 ACCTGC
DraI 126 TTTAAA TTT^AAA
1684 TTTAAA
EarI 1502 CTCTTC CTCTTC(1/4)
1868 GAAGAG
FauI 927 CCCGC CCCGC(4/6)
805 GCGGG
HaeII 88 GGCGCC [GA]GCGC^[CT]
1645 AGCGCC
Ksp632I 1502 CTCTTC CTCTTC(1/4)
1868 GAAGAG
MaeIII 64 GTAAC ^GT[ACGT]AC
389 GTTAC
MamI 538 GATGGTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
859 GATTTCCATC
MslI 274 CATCATGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
277 CATGGTGATG
SspI 114 AATATT AAT^ATT
1095 AATATT
StuI 1058 AGGCCT AGG^CCT
1743 AGGCCT
#########################################
# 3 OR MORE CUT ENZYMES ( 60 enzymes) #
#########################################
AciI AfaI AluI AlwI AvaII
BalI BamHI BbvI BfaI BsaJI
BsiYI BslI BsmAI BssKI BstF5I
BstNI BstYI Cac8I CfoI Cfr13I
DdeI DpnII EaeI EcoRII Fnu4HI
FokI HaeIII HhaI HinP1I HinfI
HphI ItaI MaeI MboI MboII
MflI MluNI MnlI MscI MseI
MvaI MwoI NdeII NlaIII NlaIV
NspI PleI RsaI Sau3AI Sau96I
ScrFI SfaNI SmlI TaqI Tru9I
TseI Tsp509I TspRI TthHB8I XhoII