##################################
# NO CUT ENZYMES ( 111 enzymes) #
##################################
AatII AccI AccIII AclI AcsI
AflII AflIII AgeI AhdI Alw44I
AocI ApaLI ApoI AscI AseI
AsnI Asp700I AspEI AspI AvrII
BbrPI BciVI BclI BfrI BglII
BlnI BlpI Bpu1102I BsaAI BsaBI
BsaI BsaWI BseAI BsiWI BsmI
Bsp1407I BspEI BspHI BspLU11I BsrBI
BsrFI BsrGI BssHII BssSI Bst1107I
BstBI BstZ17I Bsu36I CelII Cfr10I
CpoI DraI EagI Eam1105I EclXI
Eco52I Eco81I EcoNI EcoRI EcoRV
EcoT22I FbaI FseI HindIII HpaI
MamI MfeI MluI MroI MunI
NaeI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SacI SalI ScaI SexAI
SfiI SfuI SgrAI SmaI SnaBI
SpeI SspBI SspI SwaI TfiI
Tth111I XbaI XhoI XmaCI XmaI
XmnI
###################################
# ONE CUT ENZYMES ( 44 enzymes) #
###################################
Acc65I 642 GGTACC G^GTACC
Asp718I 642 GGTACC G^GTACC
AspHI 875 GTGCTC G[AT]GC[AT]^C
AviII 943 TGCGCA TGC^GCA
BamHI 1662 GGATCC G^GATCC
BbeI 842 GGCGCC GGCGC^C
BbsI 216 GTCTTC GAAGAC(2/6)
BglI 1106 GCCTGGAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI 216 GTCTTC GAAGAC(2/6)
BsiEI 262 CGGTCG CG[GA][CT]^CG
BsiHKAI 875 GTGCTC G[AT]GC[AT]^C
BspDI 1467 ATCGAT AT^CGAT
BsrDI 959 GCAATG GCAATG(2/0)
BstEII 746 GGTCACC G^GT[ACGT]ACC
BstPI 746 GGTCACC G^GT[ACGT]ACC
BstYI 1662 GGATCC [GA]^GATC[CT]
ClaI 1467 ATCGAT AT^CGAT
DraIII 947 CACAGTGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 1254 GACATCCGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 746 GGTCACC G^GT[ACGT]ACC
FspI 943 TGCGCA TGC^GCA
HincII 309 GTCAAC GT[CT]^[GA]AC
HindII 309 GTCAAC GT[CT]^[GA]AC
KasI 842 GGCGCC G^GCGCC
KpnI 642 GGTACC GGTAC^C
KspI 437 CCGCGG CCGC^GG
MaeII 940 ACGT A^CGT
MflI 1662 GGATCC [GA]^GATC[CT]
MseI 1900 TTAA T^TAA
NarI 842 GGCGCC GG^CGCC
PstI 741 CTGCAG CTGCA^G
SacII 437 CCGCGG CCGC^GG
SapI 1121 GCTCTTC GCTCTTC(1/4)
SbfI 740 CCTGCAGG CCTGCA^GG
SfcI 741 CTGCAG C^T[GA][CT]AG
SfoI 842 GGCGCC GGC^GCC
SphI 347 GCATGC GCATG^C
Sse8387I 740 CCTGCAGG CCTGCA^GG
StuI 1718 AGGCCT AGG^CCT
TaqI 1468 TCGA T^CGA
Tru9I 1900 TTAA T^TAA
TthHB8I 1468 TCGA T^CGA
XcmI 145 CCACCTTTGCCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 1662 GGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AccII 438 CGCG CG^CG
933 CGCG
AcyI 842 GGCGCC G[GA]^CG[CT]C
1171 GGCGTC
AfaI 643 GTAC GT^AC
1304 GTAC
Aor51HI 424 AGCGCT AGC^GCT
925 AGCGCT
BbvCI 47 CCTCAGC CCTCAGC(-5/-2)
320 CCTCAGC
BpmI 1789 CTCCAG CTGGAG(16/14)
84 CTGGAG
BsaHI 842 GGCGCC G[GA]^CG[CT]C
1171 GGCGTC
BsgI 1230 CTGCAC GTGCAG(16/14)
159 GTGCAG
BsmBI 1613 GAGACG CGTCTC(1/5)
1803 GAGACG
BspMI 252 ACCTGC ACCTGC(4/8)
743 GCAGGT
BstAPI 338 GCAGGCACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
342 GCACTGCATGC
BstUI 438 CGCG CG^CG
933 CGCG
BstXI 670 CCATTGTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
802 CCATCTTGGTGG
EarI 1122 CTCTTC CTCTTC(1/4)
1347 GAAGAG
Eco47III 424 AGCGCT AGC^GCT
925 AGCGCT
Hin1I 842 GGCGCC G[GA]^CG[CT]C
1171 GGCGTC
Ksp632I 1122 CTCTTC CTCTTC(1/4)
1347 GAAGAG
MvnI 438 CGCG CG^CG
933 CGCG
NcoI 880 CCATGG C^CATGG
1918 CCATGG
RsaI 643 GTAC GT^AC
1304 GTAC
SmlI 828 CTCAAG C^T[CT][GA]AG
1134 CTTGAG
Tsp509I 512 AATT ^AATT
1384 AATT
#########################################
# 3 OR MORE CUT ENZYMES ( 81 enzymes) #
#########################################
AciI AluI AlwI AlwNI ApaI
AvaI AvaII BalI BanI BanII
BbvI BcgI BcnI BfaI BmyI
BsaJI BseRI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BsrI BssKI
BstF5I BstNI Cac8I CfoI Cfr13I
DdeI DpnII DraII DsaI EaeI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI HinP1I HinfI HpaII HphI
ItaI MaeI MaeIII MboI MboII
MluNI MnlI MscI MslI MspA1I
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV NspI PflMI PleI
PpuMI PvuII Sau3AI Sau96I ScrFI
SfaNI StyI TseI Tsp45I TspRI
Van91I