##################################
# NO CUT ENZYMES ( 104 enzymes) #
##################################
AatII Acc65I AclI AcyI AgeI
AhdI Alw44I AocI Aor51HI ApaLI
AscI Asp718I AspEI AspHI AspI
AviII AvrII BamHI BbeI BcgI
BclI BglII BlnI BlpI Bpu1102I
BsaBI BsaHI BsiEI BsiHKAI BsiWI
BsmBI Bsp1407I BspDI BspLU11I BspMI
BsrFI BsrGI BssHII BstEII BstPI
Bsu36I CelII Cfr10I ClaI DraIII
DrdI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoNI EcoO65I EcoRV
FbaI FseI FspI HaeII Hin1I
HincII HindII HpaI KasI KpnI
KspI MamI MluI NaeI NarI
NgoMIV NotI NruI PacI PaeR7I
PflFI PflMI PinAI PmeI PshAI
Psp1406I PstI PvuI SacI SacII
SalI SapI SbfI SexAI SfoI
SgrAI SmaI SnaBI SphI Sse8387I
SspBI SwaI Tth111I Van91I XbaI
XcmI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 52 enzymes) #
###################################
AccI 1001 GTATAC GT^[AC][GT]AC
AccIII 329 TCCGGA T^CCGGA
AflII 845 CTTAAG C^TTAAG
AlwNI 461 CAGCTGCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 108 GGGCCC GGGCC^C
BanI 2273 GGCACC G^G[CT][GA]CC
BanII 108 GGGCCC G[GA]GC[CT]^C
BfrI 845 CTTAAG C^TTAAG
BglI 2 GCCTCACTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaI 2202 GAGACC GGTCTC(1/5)
BsaWI 329 TCCGGA [AT]^CCGG[AT]
BseAI 329 TCCGGA T^CCGGA
BsgI 2364 CTGCAC GTGCAG(16/14)
BsmI 1877 GAATGC GAATGC(1/-1)
BspEI 329 TCCGGA T^CCGGA
BssSI 96 CTCGTG CACGAG(-5/-1)
Bst1107I 1001 GTATAC GTA^TAC
BstAPI 2333 GCAGAGGTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 2004 TTCGAA TT^CGAA
BstXI 2286 CCAGCTACTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 290 GGATCT [GA]^GATC[CT]
BstZ17I 1001 GTATAC GTA^TAC
CfoI 193 GCGC GCG^C
CpoI 127 CGGACCG CG^G[AT]CCG
DraII 306 GGGACCT [GA]G^G[ACGT]CC[CT]
DsaI 477 CCATGG C^C[GA][CT]GG
EcoO109I 306 GGGACCT [GA]G^G[ACGT]CC[CT]
EcoRI 1923 GAATTC G^AATTC
EcoT22I 1421 ATGCAT ATGCA^T
HhaI 193 GCGC GCG^C
HinP1I 193 GCGC G^CGC
HindIII 1980 AAGCTT A^AGCTT
MfeI 767 CAATTG C^AATTG
MflI 290 GGATCT [GA]^GATC[CT]
MroI 329 TCCGGA T^CCGGA
MspA1I 461 CAGCTG C[AC]G^C[GT]G
MunI 767 CAATTG C^AATTG
NcoI 477 CCATGG C^CATGG
NheI 114 GCTAGC G^CTAGC
NsiI 1421 ATGCAT ATGCA^T
NspV 2004 TTCGAA TT^CGAA
PpuMI 306 GGGACCT [GA]G^G[AT]CC[CT]
PvuII 461 CAGCTG CAG^CTG
RsrII 127 CGGACCG CG^G[AT]CCG
ScaI 1404 AGTACT AGT^ACT
SfiI 1 GGCCTCACTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfuI 2004 TTCGAA TT^CGAA
SpeI 2025 ACTAGT A^CTAGT
SspI 995 AATATT AAT^ATT
TaqI 2005 TCGA T^CGA
TthHB8I 2005 TCGA T^CGA
XhoII 290 GGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 38 enzymes) #
###################################
AccII 271 CGCG CG^CG
451 CGCG
AflIII 437 ACACGT A^C[GA][CT]GT
1904 ACGTGT
AlwI 290 GGATC GGATC(4/5)
2179 GGATC
AseI 386 ATTAAT AT^TAAT
1064 ATTAAT
AsnI 386 ATTAAT AT^TAAT
1064 ATTAAT
AvaI 140 CTCGGG C^[CT]CG[GA]G
1408 CTCGGG
AvaII 128 GGACC G^G[AT]CC
307 GGACC
BalI 597 TGGCCA TGG^CCA
2212 TGGCCA
BbrPI 854 CACGTG CAC^GTG
1903 CACGTG
BbsI 2007 GAAGAC GAAGAC(2/6)
824 GTCTTC
BbvCI 2167 GCTGAGG CCTCAGC(-5/-2)
2301 GCTGAGG
BciVI 762 GGATAC GTATCC(6/5)
1654 GTATCC
BcnI 65 CCCGG CC^[GC]GG
297 CCCGG
BpuAI 2007 GAAGAC GAAGAC(2/6)
824 GTCTTC
BsaAI 854 CACGTG [CT]AC^GT[GA]
1903 CACGTG
BsoBI 140 CTCGGG C^[CT]CG[GA]G
1408 CTCGGG
BspHI 883 TCATGA T^CATGA
1927 TCATGA
BsrBI 88 CCGCTC CCGCTC(-3/-3)
122 CCGCTC
BsrDI 811 GCAATG GCAATG(2/0)
1969 GCAATG
BstUI 271 CGCG CG^CG
451 CGCG
DraI 350 TTTAAA TTT^AAA
2084 TTTAAA
EaeI 597 TGGCCA [CT]^GGCC[GA]
2212 TGGCCA
EcoT14I 477 CCATGG C^C[AT][AT]GG
685 CCAAGG
HgaI 850 GACGC GACGC(5/10)
118 GCGTC
MluNI 597 TGGCCA TGG^CCA
2212 TGGCCA
MscI 597 TGGCCA TGG^CCA
2212 TGGCCA
MslI 1032 CATTTGAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1284 CATATGAATG
MvnI 271 CGCG CG^CG
451 CGCG
NciI 65 CCCGG CC^[GC]GG
297 CCCGG
NdeI 1088 CATATG CA^TATG
1284 CATATG
PmaCI 854 CACGTG CAC^GTG
1903 CACGTG
PmlI 854 CACGTG CAC^GTG
1903 CACGTG
PshBI 386 ATTAAT AT^TAAT
1064 ATTAAT
RcaI 883 TCATGA T^CATGA
1927 TCATGA
SfaNI 574 GATGC GCATC(5/9)
266 GCATC
SfcI 562 CTACAG C^T[GA][CT]AG
1228 CTATAG
SmlI 845 CTTAAG C^T[CT][GA]AG
2185 CTTGAG
StyI 477 CCATGG C^C[AT][AT]GG
685 CCAAGG
#########################################
# 3 OR MORE CUT ENZYMES ( 64 enzymes) #
#########################################
AciI AcsI AfaI AluI ApoI
Asp700I BbvI BfaI BmyI BpmI
BsaJI BseRI BsiYI BslI BsmAI
BsmFI Bsp1286I BsrI BssKI BstF5I
BstNI Cac8I Cfr13I DdeI DpnII
EarI EcoRII FauI Fnu4HI FokI
HaeIII HapII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MnlI MseI MspI
MvaI MwoI NdeII NlaIII NlaIV
NspI PleI RsaI Sau3AI Sau96I
ScrFI StuI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI XmnI