##################################
# NO CUT ENZYMES ( 101 enzymes) #
##################################
AatII Acc65I AclI AcyI AflII
AgeI AhdI AseI AsnI Asp700I
Asp718I AspEI AspI AvaI AviII
BbeI BciVI BfrI BglII BlpI
Bpu1102I BsaHI BsgI BsiEI BsiWI
BsmI BsoBI Bsp1407I BsrDI BsrGI
Bst1107I BstAPI BstBI BstEII BstPI
BstZ17I CelII CpoI DraI DrdI
EagI Eam1105I EclXI Eco52I EcoO65I
EcoRI EcoRV FseI FspI Hin1I
HincII HindII HindIII HpaI KasI
KpnI KspI MfeI MluI MunI
NaeI NarI NdeI NgoMIV NheI
NotI NruI NspV PacI PaeR7I
PflFI PflMI PinAI PmeI PshBI
Psp1406I PvuI RsrII SacII SalI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
Sse8387I SspBI SspI SwaI Tth111I
Van91I XbaI XhoI XmaCI XmaI
XmnI
###################################
# ONE CUT ENZYMES ( 48 enzymes) #
###################################
AccI 726 GTAGAC GT^[AC][GT]AC
AccIII 349 TCCGGA T^CCGGA
AcsI 2036 AAATTT [GA]^AATT[CT]
Alw44I 1117 GTGCAC G^TGCAC
Aor51HI 433 AGCGCT AGC^GCT
ApaLI 1117 GTGCAC G^TGCAC
ApoI 2036 AAATTT [GA]^AATT[CT]
AscI 785 GGCGCGCC GG^CGCGCC
AvrII 1429 CCTAGG C^CTAGG
BamHI 346 GGATCC G^GATCC
BanI 1548 GGTGCC G^G[CT][GA]CC
BbvCI 1458 CCTCAGC CCTCAGC(-5/-2)
BcgI 832 GCATCGTCATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BlnI 1429 CCTAGG C^CTAGG
BsaBI 806 GATCATCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 82 GAGACC GGTCTC(1/5)
BsaWI 349 TCCGGA [AT]^CCGG[AT]
BseAI 349 TCCGGA T^CCGGA
BsmBI 591 GAGACG CGTCTC(1/5)
BspDI 1715 ATCGAT AT^CGAT
BspEI 349 TCCGGA T^CCGGA
BspHI 802 TCATGA T^CATGA
BspLU11I 646 ACATGT A^CATGT
BsrBI 326 CCGCTC CCGCTC(-3/-3)
BsrFI 471 ACCGGC [GA]^CCGG[CT]
BssHII 786 GCGCGC G^CGCGC
BssSI 669 CTCGTG CACGAG(-5/-1)
BstYI 346 GGATCC [GA]^GATC[CT]
Cfr10I 471 ACCGGC [GA]^CCGG[CT]
ClaI 1715 ATCGAT AT^CGAT
DraIII 1306 CACATGGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco47III 433 AGCGCT AGC^GCT
EcoNI 1529 CCTCTGGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT22I 1865 ATGCAT ATGCA^T
HaeII 433 AGCGCT [GA]GCGC^[CT]
MamI 806 GATCATCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 346 GGATCC [GA]^GATC[CT]
MroI 349 TCCGGA T^CCGGA
MseI 282 TTAA T^TAA
NsiI 1865 ATGCAT ATGCA^T
PshAI 1949 GACCACTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 802 TCATGA T^CATGA
SacI 24 GAGCTC GAGCT^C
SapI 1207 GCTCTTC GCTCTTC(1/4)
SmlI 1607 CTCAAG C^T[CT][GA]AG
Tru9I 282 TTAA T^TAA
Tsp509I 2037 AATT ^AATT
XhoII 346 GGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AflIII 646 ACATGT A^C[GA][CT]GT
1737 ACGTGT
ApaI 1017 GGGCCC GGGCC^C
1194 GGGCCC
BalI 661 TGGCCA TGG^CCA
1302 TGGCCA
BbrPI 1725 CACGTG CAC^GTG
1736 CACGTG
BclI 805 TGATCA T^GATCA
1377 TGATCA
BcnI 988 CCCGG CC^[GC]GG
1768 CCGGG
BsaAI 1725 CACGTG [CT]AC^GT[GA]
1736 CACGTG
BspMI 1243 ACCTGC ACCTGC(4/8)
110 GCAGGT
BsrI 487 CCAGT ACTGG(1/-1)
1938 CCAGT
BstXI 664 CCATGCTCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
933 CCACCTGGCTGG
EarI 1208 CTCTTC CTCTTC(1/4)
1077 GAAGAG
FbaI 805 TGATCA T^GATCA
1377 TGATCA
HgaI 593 GACGC GACGC(5/10)
1065 GCGTC
Ksp632I 1208 CTCTTC CTCTTC(1/4)
1077 GAAGAG
MluNI 661 TGGCCA TGG^CCA
1302 TGGCCA
MscI 661 TGGCCA TGG^CCA
1302 TGGCCA
NciI 988 CCCGG CC^[GC]GG
1768 CCGGG
NcoI 1013 CCATGG C^CATGG
1404 CCATGG
PleI 555 GACTC GAGTC(4/5)
1364 GAGTC
PmaCI 1725 CACGTG CAC^GTG
1736 CACGTG
PmlI 1725 CACGTG CAC^GTG
1736 CACGTG
PstI 1594 CTGCAG CTGCA^G
1683 CTGCAG
SfcI 1594 CTGCAG C^T[GA][CT]AG
1683 CTGCAG
SphI 1867 GCATGC GCATG^C
1877 GCATGC
StuI 317 AGGCCT AGG^CCT
1679 AGGCCT
TfiI 122 GATTC G^A[AT]TC
1980 GATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 83 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AlwNI AocI AspHI AvaII BanII
BbsI BbvI BfaI BglI BmyI
BpmI BpuAI BsaJI BseRI BsiHKAI
BsiYI BslI BsmAI BsmFI Bsp1286I
BssKI BstF5I BstNI BstUI Bsu36I
Cac8I CfoI Cfr13I DdeI DpnII
DraII DsaI EaeI Eco81I EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeIII HapII HhaI HinP1I HinfI
HpaII HphI ItaI MaeI MaeII
MaeIII MboI MboII MnlI MslI
MspA1I MspI MvaI MvnI MwoI
NdeII NlaIII NlaIV NspI PpuMI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI StyI TaqI TseI Tsp45I
TspRI TthHB8I XcmI