################################## # NO CUT ENZYMES ( 101 enzymes) # ################################## Acc65I AccI AflII AflIII AgeI AhdI Alw44I Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AviII AvrII BalI BamHI BanII BbsI BbvCI BcgI BclI BfrI BglII BlnI BlpI BpmI Bpu1102I BpuAI BsaBI BsaI BsiHKAI BsiWI Bsp1407I BspDI BspLU11I BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstZ17I CelII Cfr10I ClaI CpoI DraI Eam1105I Eco47III EcoRV EcoT22I FbaI FseI FspI HgaI KpnI MamI MfeI MluI MluNI MscI MunI NaeI NgoMIV NotI NruI NsiI PacI PaeR7I PflFI PflMI PinAI PmeI PshAI PshBI PvuI RsrII SacI SalI SapI SbfI SfiI SgrAI SnaBI SpeI Sse8387I SspBI StuI SwaI Tth111I Van91I XhoI XmnI ################################### # ONE CUT ENZYMES ( 38 enzymes) # ################################### AatII 475 GACGTC GACGT^C AclI 775 AACGTT AA^CGTT BbrPI 1645 CACGTG CAC^GTG BciVI 353 GGATAC GTATCC(6/5) BglI 2224 GCCCCAGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BsgI 1788 CTGCAC GTGCAG(16/14) BsiEI 1964 CGGCCG CG[GA][CT]^CG BsmBI 1214 CGTCTC CGTCTC(1/5) BspHI 1088 TCATGA T^CATGA BstBI 538 TTCGAA TT^CGAA BstXI 1222 CCATATTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG DraIII 1310 CACTCAGTG CAC[ACGT][ACGT][ACGT]^GTG DrdI 1320 GACAGAGAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EaeI 1964 CGGCCG [CT]^GGCC[GA] EagI 1964 CGGCCG C^GGCCG EclXI 1964 CGGCCG C^GGCCG Eco52I 1964 CGGCCG C^GGCCG EcoRI 550 GAATTC G^AATTC HindIII 467 AAGCTT A^AGCTT HpaI 675 GTTAAC GTT^AAC NdeI 2288 CATATG CA^TATG NheI 294 GCTAGC G^CTAGC NspI 207 GCATGC [GA]CATG^[CT] NspV 538 TTCGAA TT^CGAA PmaCI 1645 CACGTG CAC^GTG PmlI 1645 CACGTG CAC^GTG Psp1406I 775 AACGTT AA^CGTT PvuII 910 CAGCTG CAG^CTG RcaI 1088 TCATGA T^CATGA ScaI 1784 AGTACT AGT^ACT SfuI 538 TTCGAA TT^CGAA SmaI 157 CCCGGG CCC^GGG SmlI 1803 CTCAAG C^T[CT][GA]AG SphI 207 GCATGC GCATG^C XbaI 2320 TCTAGA T^CTAGA XcmI 1721 CCATTGTGTCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmaCI 157 CCCGGG C^CCGGG XmaI 157 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 31 enzymes) # ################################### AccIII 821 TCCGGA T^CCGGA 2235 TCCGGA AocI 1541 CCTCAGG CC^T[ACGT]AGG 1863 CCTAAGG BbeI 232 GGCGCC GGCGC^C 361 GGCGCC BfaI 295 CTAG C^TAG 2321 CTAG BsaAI 1645 CACGTG [CT]AC^GT[GA] 2014 TACGTG BseAI 821 TCCGGA T^CCGGA 2235 TCCGGA BsmI 408 GAATGC GAATGC(1/-1) 2324 GAATGC BspEI 821 TCCGGA T^CCGGA 2235 TCCGGA BspMI 341 ACCTGC ACCTGC(4/8) 1744 ACCTGC Bsu36I 1541 CCTCAGG CC^T[ACGT]AGG 1863 CCTAAGG EarI 1123 CTCTTC CTCTTC(1/4) 1435 GAAGAG Eco81I 1541 CCTCAGG CC^T[ACGT]AGG 1863 CCTAAGG EcoNI 730 CCTGAAACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 1990 CCTTATTTAGG EcoT14I 1286 CCATGG C^C[AT][AT]GG 2159 CCATGG HincII 675 GTTAAC GT[CT]^[GA]AC 1329 GTCAAC HindII 675 GTTAAC GT[CT]^[GA]AC 1329 GTCAAC KasI 232 GGCGCC G^GCGCC 361 GGCGCC Ksp632I 1123 CTCTTC CTCTTC(1/4) 1435 GAAGAG KspI 3 CCGCGG CCGC^GG 57 CCGCGG MaeI 295 CTAG C^TAG 2321 CTAG MroI 821 TCCGGA T^CCGGA 2235 TCCGGA NarI 232 GGCGCC GG^CGCC 361 GGCGCC NcoI 1286 CCATGG C^CATGG 2159 CCATGG PstI 250 CTGCAG CTGCA^G 1244 CTGCAG SacII 3 CCGCGG CCGC^GG 57 CCGCGG SexAI 1375 ACCTGGT A^CC[AT]GGT 1847 ACCAGGT SfoI 232 GGCGCC GGC^GCC 361 GGCGCC StyI 1286 CCATGG C^C[AT][AT]GG 2159 CCATGG TaqI 539 TCGA T^CGA 1560 TCGA TfiI 520 GAATC G^A[AT]TC 1081 GAATC TthHB8I 539 TCGA T^CGA 1560 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 88 enzymes) # ######################################### AccII AciI AcsI AcyI AfaI AluI AlwI AlwNI ApoI AvaI AvaII BanI BbvI BcnI BmyI BsaHI BsaJI BsaWI BseRI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BsrI BssKI BstEII BstF5I BstNI BstPI BstUI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraII DsaI EcoO109I EcoO65I EcoRII FauI Fnu4HI FokI HaeII HaeIII HapII HhaI Hin1I HinP1I HinfI HpaII HphI ItaI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV PleI PpuMI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SspI Tru9I TseI Tsp45I Tsp509I TspRI XhoII