##################################
# NO CUT ENZYMES ( 101 enzymes) #
##################################
Acc65I AccI AflII AflIII AgeI
AhdI Alw44I Aor51HI ApaI ApaLI
AscI AseI AsnI Asp700I Asp718I
AspEI AspHI AspI AviII AvrII
BalI BamHI BanII BbsI BbvCI
BcgI BclI BfrI BglII BlnI
BlpI BpmI Bpu1102I BpuAI BsaBI
BsaI BsiHKAI BsiWI Bsp1407I BspDI
BspLU11I BsrBI BsrDI BsrFI BsrGI
BssHII BssSI Bst1107I BstAPI BstZ17I
CelII Cfr10I ClaI CpoI DraI
Eam1105I Eco47III EcoRV EcoT22I FbaI
FseI FspI HgaI KpnI MamI
MfeI MluI MluNI MscI MunI
NaeI NgoMIV NotI NruI NsiI
PacI PaeR7I PflFI PflMI PinAI
PmeI PshAI PshBI PvuI RsrII
SacI SalI SapI SbfI SfiI
SgrAI SnaBI SpeI Sse8387I SspBI
StuI SwaI Tth111I Van91I XhoI
XmnI
###################################
# ONE CUT ENZYMES ( 38 enzymes) #
###################################
AatII 475 GACGTC GACGT^C
AclI 775 AACGTT AA^CGTT
BbrPI 1645 CACGTG CAC^GTG
BciVI 353 GGATAC GTATCC(6/5)
BglI 2224 GCCCCAGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsgI 1788 CTGCAC GTGCAG(16/14)
BsiEI 1964 CGGCCG CG[GA][CT]^CG
BsmBI 1214 CGTCTC CGTCTC(1/5)
BspHI 1088 TCATGA T^CATGA
BstBI 538 TTCGAA TT^CGAA
BstXI 1222 CCATATTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DraIII 1310 CACTCAGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 1320 GACAGAGAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 1964 CGGCCG [CT]^GGCC[GA]
EagI 1964 CGGCCG C^GGCCG
EclXI 1964 CGGCCG C^GGCCG
Eco52I 1964 CGGCCG C^GGCCG
EcoRI 550 GAATTC G^AATTC
HindIII 467 AAGCTT A^AGCTT
HpaI 675 GTTAAC GTT^AAC
NdeI 2288 CATATG CA^TATG
NheI 294 GCTAGC G^CTAGC
NspI 207 GCATGC [GA]CATG^[CT]
NspV 538 TTCGAA TT^CGAA
PmaCI 1645 CACGTG CAC^GTG
PmlI 1645 CACGTG CAC^GTG
Psp1406I 775 AACGTT AA^CGTT
PvuII 910 CAGCTG CAG^CTG
RcaI 1088 TCATGA T^CATGA
ScaI 1784 AGTACT AGT^ACT
SfuI 538 TTCGAA TT^CGAA
SmaI 157 CCCGGG CCC^GGG
SmlI 1803 CTCAAG C^T[CT][GA]AG
SphI 207 GCATGC GCATG^C
XbaI 2320 TCTAGA T^CTAGA
XcmI 1721 CCATTGTGTCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 157 CCCGGG C^CCGGG
XmaI 157 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AccIII 821 TCCGGA T^CCGGA
2235 TCCGGA
AocI 1541 CCTCAGG CC^T[ACGT]AGG
1863 CCTAAGG
BbeI 232 GGCGCC GGCGC^C
361 GGCGCC
BfaI 295 CTAG C^TAG
2321 CTAG
BsaAI 1645 CACGTG [CT]AC^GT[GA]
2014 TACGTG
BseAI 821 TCCGGA T^CCGGA
2235 TCCGGA
BsmI 408 GAATGC GAATGC(1/-1)
2324 GAATGC
BspEI 821 TCCGGA T^CCGGA
2235 TCCGGA
BspMI 341 ACCTGC ACCTGC(4/8)
1744 ACCTGC
Bsu36I 1541 CCTCAGG CC^T[ACGT]AGG
1863 CCTAAGG
EarI 1123 CTCTTC CTCTTC(1/4)
1435 GAAGAG
Eco81I 1541 CCTCAGG CC^T[ACGT]AGG
1863 CCTAAGG
EcoNI 730 CCTGAAACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1990 CCTTATTTAGG
EcoT14I 1286 CCATGG C^C[AT][AT]GG
2159 CCATGG
HincII 675 GTTAAC GT[CT]^[GA]AC
1329 GTCAAC
HindII 675 GTTAAC GT[CT]^[GA]AC
1329 GTCAAC
KasI 232 GGCGCC G^GCGCC
361 GGCGCC
Ksp632I 1123 CTCTTC CTCTTC(1/4)
1435 GAAGAG
KspI 3 CCGCGG CCGC^GG
57 CCGCGG
MaeI 295 CTAG C^TAG
2321 CTAG
MroI 821 TCCGGA T^CCGGA
2235 TCCGGA
NarI 232 GGCGCC GG^CGCC
361 GGCGCC
NcoI 1286 CCATGG C^CATGG
2159 CCATGG
PstI 250 CTGCAG CTGCA^G
1244 CTGCAG
SacII 3 CCGCGG CCGC^GG
57 CCGCGG
SexAI 1375 ACCTGGT A^CC[AT]GGT
1847 ACCAGGT
SfoI 232 GGCGCC GGC^GCC
361 GGCGCC
StyI 1286 CCATGG C^C[AT][AT]GG
2159 CCATGG
TaqI 539 TCGA T^CGA
1560 TCGA
TfiI 520 GAATC G^A[AT]TC
1081 GAATC
TthHB8I 539 TCGA T^CGA
1560 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 88 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AluI AlwI AlwNI ApoI AvaI
AvaII BanI BbvI BcnI BmyI
BsaHI BsaJI BsaWI BseRI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BsrI BssKI BstEII BstF5I BstNI
BstPI BstUI BstYI Cac8I CfoI
Cfr13I DdeI DpnII DraII DsaI
EcoO109I EcoO65I EcoRII FauI Fnu4HI
FokI HaeII HaeIII HapII HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI MaeII MaeIII MboI MboII
MflI MnlI MseI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV PleI PpuMI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SspI Tru9I TseI Tsp45I
Tsp509I TspRI XhoII