##################################
# NO CUT ENZYMES ( 99 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AgeI Alw44I ApaLI AscI AseI
AsnI Asp700I Asp718I AspI BalI
BbrPI BciVI BclI BglII BsaAI
BsaBI BsaWI BseAI BsgI BsiEI
BsiWI BsmBI BsmI BspDI BspEI
BspHI BsrBI BsrDI BssHII BssSI
Bst1107I BstBI BstEII BstPI BstZ17I
ClaI CpoI DraI EaeI EagI
EclXI Eco52I EcoO65I EcoRV EcoT22I
FbaI FseI HincII HindII HindIII
HpaI KpnI KspI MamI MfeI
MluI MluNI MroI MscI MunI
NcoI NdeI NotI NruI NsiI
NspV PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacII
SalI SapI ScaI SexAI SfuI
SgrAI SnaBI SpeI SphI SwaI
Tth111I XcmI XhoI XmnI
###################################
# ONE CUT ENZYMES ( 47 enzymes) #
###################################
AccII 1 CGCG CG^CG
AcsI 638 GAATTC [GA]^AATT[CT]
AfaI 1699 GTAC GT^AC
AflII 1890 CTTAAG C^TTAAG
AflIII 1089 ACATGT A^C[GA][CT]GT
AhdI 275 GACTTCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 831 AGCGCT AGC^GCT
ApoI 638 GAATTC [GA]^AATT[CT]
AspEI 275 GACTTCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 1389 TGCGCA TGC^GCA
AvrII 1569 CCTAGG C^CTAGG
BbeI 414 GGCGCC GGCGC^C
BcgI 348 GCATCGGCATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI 1890 CTTAAG C^TTAAG
BglI 5 GCCCCTGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 1569 CCTAGG C^CTAGG
Bsp1407I 1698 TGTACA T^GTACA
BspLU11I 1089 ACATGT A^CATGT
BsrGI 1698 TGTACA T^GTACA
BstUI 1 CGCG CG^CG
BstXI 505 CCATGAACTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DraIII 1302 CACTTAGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 275 GACTTCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 1799 CTCTTC CTCTTC(1/4)
Eco47III 831 AGCGCT AGC^GCT
EcoRI 638 GAATTC G^AATTC
EcoT14I 1569 CCTAGG C^C[AT][AT]GG
FspI 1389 TGCGCA TGC^GCA
KasI 414 GGCGCC G^GCGCC
Ksp632I 1799 CTCTTC CTCTTC(1/4)
MaeII 660 ACGT A^CGT
MvnI 1 CGCG CG^CG
NarI 414 GGCGCC GG^CGCC
NheI 1729 GCTAGC G^CTAGC
NspI 1089 ACATGT [GA]CATG^[CT]
PflMI 225 CCATATCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PvuII 377 CAGCTG CAG^CTG
RsaI 1699 GTAC GT^AC
SbfI 1030 CCTGCAGG CCTGCA^GG
SfiI 4 GGCCCCTGGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 414 GGCGCC GGC^GCC
Sse8387I 1030 CCTGCAGG CCTGCA^GG
SspBI 1698 TGTACA T^GTACA
SspI 1052 AATATT AAT^ATT
StyI 1569 CCTAGG C^C[AT][AT]GG
Van91I 225 CCATATCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 1653 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AcyI 414 GGCGCC G[GA]^CG[CT]C
1415 GACGCC
AspHI 209 GAGCTC G[AT]GC[AT]^C
516 GAGCTC
BamHI 219 GGATCC G^GATCC
669 GGATCC
BanI 414 GGCGCC G^G[CT][GA]CC
1010 GGTGCC
BbvCI 1339 GCTGAGG CCTCAGC(-5/-2)
1375 GCTGAGG
BsaHI 414 GGCGCC G[GA]^CG[CT]C
1415 GACGCC
BsaI 389 GAGACC GGTCTC(1/5)
501 GAGACC
BsiHKAI 209 GAGCTC G[AT]GC[AT]^C
516 GAGCTC
BspMI 849 ACCTGC ACCTGC(4/8)
1335 ACCTGC
BsrFI 411 GCCGGC [GA]^CCGG[CT]
1365 GCCGGC
Cfr10I 411 GCCGGC [GA]^CCGG[CT]
1365 GCCGGC
DrdI 391 GACCCTGCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1400 GACAACGAGGTC
DsaI 665 CCACGG C^C[GA][CT]GG
1662 CCGTGG
HgaI 1018 GACGC GACGC(5/10)
1415 GACGC
Hin1I 414 GGCGCC G[GA]^CG[CT]C
1415 GACGCC
NaeI 411 GCCGGC GCC^GGC
1365 GCCGGC
NgoMIV 411 GCCGGC G^CCGGC
1365 GCCGGC
PpuMI 846 AGGACCT [GA]G^G[AT]CC[CT]
1260 AGGTCCT
PstI 374 CTGCAG CTGCA^G
1031 CTGCAG
SacI 209 GAGCTC GAGCT^C
516 GAGCTC
SfcI 374 CTGCAG C^T[GA][CT]AG
1031 CTGCAG
SmaI 111 CCCGGG CCC^GGG
876 CCCGGG
Tsp45I 474 GTGAC ^GT[GC]AC
1902 GTGAC
Tsp509I 639 AATT ^AATT
1204 AATT
XmaCI 111 CCCGGG C^CCGGG
876 CCCGGG
XmaI 111 CCCGGG C^CCGGG
876 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 86 enzymes) #
#########################################
AciI AluI AlwI AlwNI AocI
ApaI AvaI AvaII BanII BbsI
BbvI BcnI BfaI BlpI BmyI
BpmI Bpu1102I BpuAI BsaJI BseRI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BsrI BssKI BstAPI BstF5I
BstNI BstYI Bsu36I Cac8I CelII
CfoI Cfr13I DdeI DpnII DraII
Eco81I EcoNI EcoO109I EcoRII FauI
Fnu4HI FokI HaeII HaeIII HapII
HhaI HinP1I HinfI HpaII HphI
ItaI MaeI MaeIII MboI MboII
MflI MnlI MseI MslI MspA1I
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV PleI Sau3AI Sau96I
ScrFI SfaNI SmlI StuI TaqI
TfiI Tru9I TseI TspRI TthHB8I
XhoII