##################################
# NO CUT ENZYMES ( 120 enzymes) #
##################################
AatII Acc65I AccIII AclI AflII
AflIII AgeI Aor51HI ApaI AscI
AseI AsnI Asp718I AspI AviII
AvrII BalI BbeI BbrPI BbsI
BbvCI BcgI BciVI BclI BfrI
BlnI BlpI Bpu1102I BpuAI BsaAI
BsaBI BsaWI BseAI BsiEI BsiWI
BsmBI BsmI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrBI BsrDI BsrGI
BssHII BssSI BstAPI BstBI BstXI
CelII ClaI CpoI DraI DrdI
EaeI EagI EclXI Eco47III Eco52I
EcoRI EcoRV EcoT22I FbaI FseI
FspI HincII HindII HindIII HpaI
KasI KpnI KspI MamI MfeI
MluI MluNI MroI MscI MunI
NarI NdeI NheI NotI NruI
NsiI NspI NspV PacI PflFI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SacII SalI SbfI ScaI SfiI
SfoI SfuI SmaI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
SwaI Tth111I XcmI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 44 enzymes) #
###################################
AccI 682 GTATAC GT^[AC][GT]AC
AccII 1339 CGCG CG^CG
AcsI 560 GAATTT [GA]^AATT[CT]
AcyI 1387 GACGCC G[GA]^CG[CT]C
AhdI 250 GACCTTTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 50 CCTCAGG CC^T[ACGT]AGG
ApoI 560 GAATTT [GA]^AATT[CT]
AspEI 250 GACCTTTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BamHI 222 GGATCC G^GATCC
BanI 1533 GGCACC G^G[CT][GA]CC
BfaI 666 CTAG C^TAG
BglI 1564 GCCCCATGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 660 AGATCT A^GATCT
BsaHI 1387 GACGCC G[GA]^CG[CT]C
BsaI 248 GAGACC GGTCTC(1/5)
Bst1107I 682 GTATAC GTA^TAC
BstEII 257 GGTCACC G^GT[ACGT]ACC
BstPI 257 GGTCACC G^GT[ACGT]ACC
BstUI 1339 CGCG CG^CG
BstZ17I 682 GTATAC GTA^TAC
Bsu36I 50 CCTCAGG CC^T[ACGT]AGG
DraIII 1863 CACATAGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 1567 CCATGG C^C[GA][CT]GG
Eam1105I 250 GACCTTTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 50 CCTCAGG CC^T[ACGT]AGG
EcoNI 1596 CCTCGAGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 257 GGTCACC G^GT[ACGT]ACC
EcoT14I 1567 CCATGG C^C[AT][AT]GG
HaeII 55 GGCGCT [GA]GCGC^[CT]
Hin1I 1387 GACGCC G[GA]^CG[CT]C
MaeI 666 CTAG C^TAG
MslI 2028 CACCACAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI 1339 CGCG CG^CG
NcoI 1567 CCATGG C^CATGG
PaeR7I 1597 CTCGAG C^TCGAG
PflMI 517 CCAGAGTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 274 AGGTCCT [GA]G^G[AT]CC[CT]
SapI 1757 GAAGAGC GCTCTTC(1/4)
SexAI 1614 ACCTGGT A^CC[AT]GGT
SgrAI 129 CGCCGGCG C[GA]^CCGG[CT]G
StyI 1567 CCATGG C^C[AT][AT]GG
Van91I 517 CCAGAGTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 665 TCTAGA T^CTAGA
XhoI 1597 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
Alw44I 902 GTGCAC G^TGCAC
1739 GTGCAC
ApaLI 902 GTGCAC G^TGCAC
1739 GTGCAC
BsgI 1238 CTGCAC GTGCAG(16/14)
836 GTGCAG
BsmAI 248 GAGAC GTCTC(1/5)
1008 GAGAC
BspMI 261 ACCTGC ACCTGC(4/8)
1190 ACCTGC
BsrFI 130 GCCGGC [GA]^CCGG[CT]
1578 GCCGGC
BstYI 222 GGATCC [GA]^GATC[CT]
660 AGATCT
CfoI 56 GCGC GCG^C
994 GCGC
Cfr10I 130 GCCGGC [GA]^CCGG[CT]
1578 GCCGGC
HgaI 1387 GACGC GACGC(5/10)
627 GCGTC
HhaI 56 GCGC GCG^C
994 GCGC
HinP1I 56 GCGC G^CGC
994 GCGC
MflI 222 GGATCC [GA]^GATC[CT]
660 AGATCT
MseI 879 TTAA T^TAA
1834 TTAA
NaeI 130 GCCGGC GCC^GGC
1578 GCCGGC
NgoMIV 130 GCCGGC G^CCGGC
1578 GCCGGC
PstI 1192 CTGCAG CTGCA^G
1653 CTGCAG
PvuII 203 CAGCTG CAG^CTG
308 CAGCTG
SacI 1516 GAGCTC GAGCT^C
1648 GAGCTC
SfcI 1192 CTGCAG C^T[GA][CT]AG
1653 CTGCAG
TaqI 382 TCGA T^CGA
1598 TCGA
Tru9I 879 TTAA T^TAA
1834 TTAA
Tsp509I 561 AATT ^AATT
811 AATT
TthHB8I 382 TCGA T^CGA
1598 TCGA
XhoII 222 GGATCC [GA]^GATC[CT]
660 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 69 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
Asp700I AspHI AvaI AvaII BanII
BbvI BcnI BmyI BpmI BsaJI
BseRI BsiHKAI BsiYI BslI BsmFI
BsoBI Bsp1286I BsrI BssKI BstF5I
BstNI Cac8I Cfr13I DdeI DpnII
DraII EarI EcoO109I EcoRII FauI
Fnu4HI FokI HaeIII HapII HinfI
HpaII HphI ItaI Ksp632I MaeII
MaeIII MboI MboII MnlI MspA1I
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV PleI RsaI Sau3AI
Sau96I ScrFI SfaNI SmlI TfiI
TseI Tsp45I TspRI XmnI