##################################
# NO CUT ENZYMES ( 111 enzymes) #
##################################
AatII Acc65I AccI AccIII AcyI
AflII AgeI Alw44I AocI ApaLI
AscI AseI AsnI Asp700I Asp718I
AviII AvrII BbeI BbrPI BbsI
BcgI BciVI BclI BfrI BlnI
BpuAI BsaAI BsaBI BsaHI BsaWI
BseAI BsiWI BspDI BspEI BspHI
BspMI BsrBI BsrDI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstZ17I Bsu36I ClaI CpoI DraI
DraIII DrdI Eco81I EcoNI EcoO65I
EcoRI EcoRV EcoT22I FbaI FseI
FspI HgaI Hin1I HindIII HpaI
KasI KpnI MamI MfeI MluI
MroI MunI NarI NdeI NheI
NotI NsiI NspV PacI PflMI
PinAI PmaCI PmeI PmlI PshAI
PshBI PvuI PvuII RcaI RsrII
SalI SapI SbfI ScaI SexAI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspI SwaI
Van91I XbaI XcmI XmaCI XmaI
XmnI
###################################
# ONE CUT ENZYMES ( 50 enzymes) #
###################################
AclI 1004 AACGTT AA^CGTT
AcsI 946 GAATTT [GA]^AATT[CT]
AflIII 754 ACATGT A^C[GA][CT]GT
AhdI 780 GACCTGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApaI 1209 GGGCCC GGGCC^C
ApoI 946 GAATTT [GA]^AATT[CT]
AspEI 780 GACCTGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 356 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 981 TGGCCA TGG^CCA
BamHI 1194 GGATCC G^GATCC
BanI 1356 GGCACC G^G[CT][GA]CC
BfaI 181 CTAG C^TAG
BglII 1275 AGATCT A^GATCT
BlpI 703 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 703 GCTCAGC GC^T[ACGT]AGC
BsaI 57 GAGACC GGTCTC(1/5)
BsiEI 748 CGGCCG CG[GA][CT]^CG
BsmBI 238 GAGACG CGTCTC(1/5)
BsmI 604 GAATGC GAATGC(1/-1)
Bsp1407I 738 TGTACA T^GTACA
BspLU11I 754 ACATGT A^CATGT
BsrGI 738 TGTACA T^GTACA
BstXI 972 CCAATGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 703 GCTCAGC GC^T[ACGT]AGC
EagI 748 CGGCCG C^GGCCG
Eam1105I 780 GACCTGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 748 CGGCCG C^GGCCG
Eco52I 748 CGGCCG C^GGCCG
HincII 1001 GTCAAC GT[CT]^[GA]AC
HindII 1001 GTCAAC GT[CT]^[GA]AC
KspI 964 CCGCGG CCGC^GG
MaeI 181 CTAG C^TAG
MaeII 1005 ACGT A^CGT
MluNI 981 TGGCCA TGG^CCA
MscI 981 TGGCCA TGG^CCA
MslI 1397 CATGTGGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NcoI 1414 CCATGG C^CATGG
NruI 856 TCGCGA TCG^CGA
PaeR7I 468 CTCGAG C^TCGAG
PflFI 356 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
Psp1406I 1004 AACGTT AA^CGTT
SacII 964 CCGCGG CCGC^GG
SfiI 968 GGCCCCAATGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmlI 468 CTCGAG C^T[CT][GA]AG
SspBI 738 TGTACA T^GTACA
StuI 427 AGGCCT AGG^CCT
Tsp45I 1187 GTCAC ^GT[GC]AC
Tsp509I 947 AATT ^AATT
Tth111I 356 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 468 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
Aor51HI 472 AGCGCT AGC^GCT
953 AGCGCT
AspHI 852 GAGCTC G[AT]GC[AT]^C
1112 GAGCTC
BbvCI 1288 CCTCAGC CCTCAGC(-5/-2)
905 GCTGAGG
BsiHKAI 852 GAGCTC G[AT]GC[AT]^C
1112 GAGCTC
EaeI 748 CGGCCG [CT]^GGCC[GA]
981 TGGCCA
Eco47III 472 AGCGCT AGC^GCT
953 AGCGCT
EcoT14I 1317 CCTTGG C^C[AT][AT]GG
1414 CCATGG
MspA1I 964 CCGCGG C[AC]G^C[GT]G
1291 CAGCGG
NaeI 866 GCCGGC GCC^GGC
1302 GCCGGC
NgoMIV 866 GCCGGC G^CCGGC
1302 GCCGGC
NspI 754 ACATGT [GA]CATG^[CT]
1396 GCATGT
PstI 447 CTGCAG CTGCA^G
1310 CTGCAG
SacI 852 GAGCTC GAGCT^C
1112 GAGCTC
SfcI 447 CTGCAG C^T[GA][CT]AG
1310 CTGCAG
StyI 1317 CCTTGG C^C[AT][AT]GG
1414 CCATGG
TaqI 469 TCGA T^CGA
1436 TCGA
TfiI 1127 GAATC G^A[AT]TC
1261 GAATC
TthHB8I 469 TCGA T^CGA
1436 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 79 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AlwNI AvaI AvaII BanII BbvI
BcnI BglI BmyI BpmI BsaJI
BseRI BsgI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BsrFI BsrI
BssKI BstF5I BstNI BstUI BstYI
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraII DsaI EarI EcoO109I
EcoRII FauI Fnu4HI FokI HaeII
HaeIII HapII HhaI HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeIII
MboI MboII MflI MnlI MseI
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV PleI PpuMI
RsaI Sau3AI Sau96I ScrFI SfaNI
Tru9I TseI TspRI XhoII