##################################
# NO CUT ENZYMES ( 86 enzymes) #
##################################
AatII Acc65I AccI AclI AcsI
AflIII AgeI AhdI Alw44I ApaLI
ApoI AscI AseI AsnI Asp718I
AspEI AspI AviII BamHI BciVI
BglII BlpI Bpu1102I BsaBI BsiEI
BsiWI BsmBI BspHI BspLU11I BsrDI
BsrFI BssSI Bst1107I BstBI BstEII
BstPI BstZ17I CelII Cfr10I CpoI
DraIII EagI Eam1105I EclXI Eco52I
EcoO65I EcoRI EcoRV FseI FspI
HindIII HpaI KpnI KspI MamI
MluI NaeI NdeI NgoMIV NotI
NruI NspV PacI PflFI PinAI
PmeI PshAI PshBI Psp1406I PvuI
RcaI RsrII SacI SacII SalI
SbfI ScaI SfiI SfuI SgrAI
SnaBI SpeI Sse8387I SwaI Tth111I
XbaI
###################################
# ONE CUT ENZYMES ( 60 enzymes) #
###################################
AccIII 15 TCCGGA T^CCGGA
AcyI 1594 GGCGCC G[GA]^CG[CT]C
AflII 1399 CTTAAG C^TTAAG
AocI 1313 CCTGAGG CC^T[ACGT]AGG
Aor51HI 1238 AGCGCT AGC^GCT
Asp700I 1886 GAATCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvrII 1326 CCTAGG C^CTAGG
BbeI 1594 GGCGCC GGCGC^C
BbrPI 969 CACGTG CAC^GTG
BbsI 725 GTCTTC GAAGAC(2/6)
BbvCI 1965 GCTGAGG CCTCAGC(-5/-2)
BcgI 1740 GCAGTTCAGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI 1399 CTTAAG C^TTAAG
BlnI 1326 CCTAGG C^CTAGG
BpmI 794 CTCCAG CTGGAG(16/14)
BpuAI 725 GTCTTC GAAGAC(2/6)
BsaHI 1594 GGCGCC G[GA]^CG[CT]C
BsaI 1950 GAGACC GGTCTC(1/5)
BsaWI 15 TCCGGA [AT]^CCGG[AT]
BseAI 15 TCCGGA T^CCGGA
BsgI 972 GTGCAG GTGCAG(16/14)
Bsp1407I 462 TGTACA T^GTACA
BspDI 427 ATCGAT AT^CGAT
BspEI 15 TCCGGA T^CCGGA
BsrBI 1284 CCGCTC CCGCTC(-3/-3)
BsrGI 462 TGTACA T^GTACA
BstAPI 1542 GCAGCCTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Bsu36I 1313 CCTGAGG CC^T[ACGT]AGG
ClaI 427 ATCGAT AT^CGAT
DraI 2030 TTTAAA TTT^AAA
DrdI 1721 GACAAGTAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 1238 AGCGCT AGC^GCT
Eco81I 1313 CCTGAGG CC^T[ACGT]AGG
EcoNI 911 CCTCCCCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT22I 740 ATGCAT ATGCA^T
Hin1I 1594 GGCGCC G[GA]^CG[CT]C
HincII 1055 GTTGAC GT[CT]^[GA]AC
HindII 1055 GTTGAC GT[CT]^[GA]AC
KasI 1594 GGCGCC G^GCGCC
MfeI 591 CAATTG C^AATTG
MroI 15 TCCGGA T^CCGGA
MslI 1607 CACATCTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MunI 591 CAATTG C^AATTG
NarI 1594 GGCGCC GG^CGCC
NcoI 35 CCATGG C^CATGG
NheI 1433 GCTAGC G^CTAGC
NsiI 740 ATGCAT ATGCA^T
PaeR7I 1244 CTCGAG C^TCGAG
PflMI 1900 CCACCCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 670 GAGTC GAGTC(4/5)
PmaCI 969 CACGTG CAC^GTG
PmlI 969 CACGTG CAC^GTG
SapI 924 GAAGAGC GCTCTTC(1/4)
SexAI 1263 ACCTGGT A^CC[AT]GGT
SfoI 1594 GGCGCC GGC^GCC
SspBI 462 TGTACA T^GTACA
SspI 2042 AATATT AAT^ATT
Van91I 1900 CCACCCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 1244 CTCGAG C^TCGAG
XmnI 1886 GAATCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AfaI 463 GTAC GT^AC
569 GTAC
AlwNI 1543 CAGCCTCTG CAG[ACGT][ACGT][ACGT]^CTG
1915 CAGCCTCTG
ApaI 1318 GGGCCC GGGCC^C
1462 GGGCCC
AspHI 11 GTGCTC G[AT]GC[AT]^C
1614 GTGCTC
BanII 1318 GGGCCC G[GA]GC[CT]^C
1462 GGGCCC
BclI 191 TGATCA T^GATCA
410 TGATCA
BsaAI 358 CACGTA [CT]AC^GT[GA]
969 CACGTG
BsiHKAI 11 GTGCTC G[AT]GC[AT]^C
1614 GTGCTC
BsmAI 1950 GAGAC GTCTC(1/5)
672 GTCTC
BsmI 742 GCATTC GAATGC(1/-1)
1015 GCATTC
BsrI 1059 ACTGG ACTGG(1/-1)
1135 ACTGG
BssHII 537 GCGCGC G^CGCGC
539 GCGCGC
BstXI 125 CCAAGGAGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1154 CCATCTGATTGG
DsaI 35 CCATGG C^C[GA][CT]GG
1582 CCGTGG
FauI 1225 CCCGC CCCGC(4/6)
1573 CCCGC
FbaI 191 TGATCA T^GATCA
410 TGATCA
HgaI 354 GCGTC GACGC(5/10)
1551 GCGTC
HphI 1586 GGTGA GGTGA(8/7)
119 TCACC
MseI 1400 TTAA T^TAA
2031 TTAA
NspI 929 GCATGC [GA]CATG^[CT]
946 GCATGC
PpuMI 885 GGGACCT [GA]G^G[AT]CC[CT]
1867 GGGACCT
PstI 181 CTGCAG CTGCA^G
1209 CTGCAG
RsaI 463 GTAC GT^AC
569 GTAC
SphI 929 GCATGC GCATG^C
946 GCATGC
StuI 80 AGGCCT AGG^CCT
733 AGGCCT
Tru9I 1400 TTAA T^TAA
2031 TTAA
XcmI 122 CCACCAAGGAGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
981 CCAGCAGCCTGTTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 85 enzymes) #
#########################################
AccII AciI AluI AlwI AvaI
AvaII BalI BanI BbvI BcnI
BfaI BglI BmyI BsaJI BseRI
BsiYI BslI BsmFI BsoBI Bsp1286I
BspMI BssKI BstF5I BstNI BstUI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII DraII EaeI EarI EcoO109I
EcoRII EcoT14I Fnu4HI FokI HaeII
HaeIII HapII HhaI HinP1I HinfI
HpaII ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MspA1I MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV PvuII Sau3AI Sau96I ScrFI
SfaNI SfcI SmaI SmlI StyI
TaqI TfiI TseI Tsp45I Tsp509I
TspRI TthHB8I XhoII XmaCI XmaI